Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,859,535	A→G	G194G (GGT→GGC)	yidK ←	putative transporter YidK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,859,535	0	A	G	100.0%	126.1 / NA	37	G194G (GGT→GGC)	yidK	putative transporter YidK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/11);  total (26/11)

GGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAA  >  NC_000913/3859400‑3859671                                                                                                                                         |                                                                                                                                         ggtcggGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGt                                                                                                                                      >  2:533710/1‑140 (MQ=255)          ccggtttGATTTTATTTTCGGGGCGTGAGGGGGGGTGAGTTCCACAATCCCCTGAAAAACGCTGCCCTCCCCCACCGCGATAAGCCAAAACCCCGCCCCCACCAC‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGt                                                                                                                             <  2:595732/135‑1 (MQ=255)              tttgtttttATTTCCTGGGCGTGAGGGGTGGTGAGTTGCTCACTCCCCTGCAAAAACCTCCCTTCCCCCATCGCGTTGAGGCAAAACCCCGCCCCCACCAC‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGc                                                                                                                         <  2:773345/134‑1 (MQ=255)                                               aGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGc                                                                                        >  1:627781/1‑140 (MQ=255)                                                 ttGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCAt                                                                                      >  1:693639/1‑140 (MQ=255)                                                    cTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCAtctg                                                                                   >  1:769177/1‑137 (MQ=255)                                                    cTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACa                                                                                   >  1:672344/1‑140 (MQ=255)                                                         tGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAAtt                                                                              >  1:468125/1‑140 (MQ=255)                                                              tgaaaaaaGCCCCCCTTCCCCATGGCATTTAGGCAAAATCCCGGCCCCATCAC‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGc                                                                         <  1:533710/135‑1 (MQ=255)                                                                cATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCa                                                                       >  2:158306/1‑140 (MQ=255)                                                                   aaaGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGAc                                                                    >  1:117510/1‑140 (MQ=255)                                                                   aaaGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGAc                                                                    >  2:1066510/1‑140 (MQ=255)                                                                               gCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTa                                                        >  1:340345/1‑140 (MQ=255)                                                                                       cgATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGc                                                >  1:933550/1‑140 (MQ=255)                                                                                          ttacaaaaaaaACCCGCCCCAACAACCCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCa                                              <  2:340345/133‑1 (MQ=255)                                                                                                  aaTACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGc                                     >  1:1087247/1‑140 (MQ=255)                                                                                                        ggCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTg                               >  2:1132634/1‑140 (MQ=255)                                                                                                         gCACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTga                              >  1:1028118/1‑140 (MQ=255)                                                                                                          cACCATCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTgag                             >  2:108157/1‑140 (MQ=255)                                                                                                            ccaccaC‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGaa                           <  1:4437/140‑1 (MQ=255)                                                                                                               tCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCAc                                   <  2:471531/129‑1 (MQ=255)                                                                                                               tCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCAc                                   >  1:471531/1‑129 (MQ=255)                                                                                                               tCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATc                        >  1:826597/1‑140 (MQ=255)                                                                                                               tCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATc                        >  1:84806/1‑140 (MQ=255)                                                                                                               tCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATc                        >  2:1100006/1‑140 (MQ=255)                                                                                                               tCAA‑CCCGCCGATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATc                        >  2:269087/1‑140 (MQ=255)                                                                                                                    cccGCCGATAACCACCCCAAGGCCGTGGATGGAATCCGCCCCTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTa                    <  2:117510/140‑1 (MQ=255)                                                                                                                     ccgccgATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAg                   <  2:126827/140‑1 (MQ=255)                                                                                                                        ccgATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGa                >  1:496580/1‑140 (MQ=255)                                                                                                                        ccgATAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGa                >  1:741914/1‑140 (MQ=255)                                                                                                                            tAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGaa                           >  1:93514/1‑125 (MQ=255)                                                                                                                            tAACCAGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGaa                           <  2:93514/125‑1 (MQ=255)                                                                                                                                 aGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTcccc          >  2:124782/1‑137 (MQ=255)                                                                                                                                 aGCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTcccc          <  1:124782/137‑1 (MQ=255)                                                                                                                                  gCCCAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACg      >  1:797580/1‑140 (MQ=255)                                                                                                                                   cccAATGTCGCTGATGGATTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAACTTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGt     >  2:679025/1‑140 (MQ=255)                                                                                                                                    ccAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTACCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTg    <  2:797580/140‑1 (MQ=255)                                                                                                                                     cAATGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGa   >  2:576464/1‑140 (MQ=255)                                                                                                                                      aaTGCCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGaa  <  1:796916/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAA‑CCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAA  >  NC_000913/3859400‑3859671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑