Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,902,262	T→C	intergenic (‑42/+27)	bglH ← / ← bglB	carbohydrate‑specific outer membrane porin, cryptic/6‑phospho‑beta‑glucosidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,902,262	0	T	C	100.0%	283.0 / NA	77	intergenic (‑42/+27)	bglH/bglB	carbohydrate‑specific outer membrane porin, cryptic/6‑phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/32);  total (45/32)

TTGTTCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC  >  NC_000913/3902125‑3902396                                                                                                                                          |                                                                                                                                       ttgttCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCAccc                                                                                                                                      >  2:10460/1‑140 (MQ=255)  tgttCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCa                                                                                                                                     >  2:842998/1‑140 (MQ=255)   gttCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAg                                                                                                                                    >  1:555184/1‑140 (MQ=255)   gttCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAg                                                                                                                                    >  1:115846/1‑140 (MQ=255)    ttCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGa                                                                                                                                   <  2:909598/140‑1 (MQ=255)    ttCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGa                                                                                                                                   <  2:260218/140‑1 (MQ=255)         ccGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGtt                                                                                                                              <  1:483889/140‑1 (MQ=255)            ttAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTg                                                                                                                           >  2:234497/1‑140 (MQ=255)              aaTGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGaa                                                                                                                         >  2:561159/1‑140 (MQ=255)                 gATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGc                                                                                                                      <  1:215931/140‑1 (MQ=255)                   ttCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGcga                                                                                                                    >  2:474301/1‑140 (MQ=255)                      aGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGtt                                                                                                                              >  1:176705/1‑127 (MQ=255)                      aGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGtt                                                                                                                              <  2:176705/127‑1 (MQ=255)                      aGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGcgacga                                                                                                                 >  2:990352/1‑140 (MQ=255)                        ccAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATa                                                                                                               >  1:135706/1‑140 (MQ=255)                             gATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGcgac                                                                                                                   >  2:1092231/1‑131 (MQ=255)                             gATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGcgac                                                                                                                   <  1:1092231/131‑1 (MQ=255)                             gATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTaa                                                                                                          <  1:213659/140‑1 (MQ=255)                               ttGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAgg                                                                                                        <  2:367490/140‑1 (MQ=255)                               ttGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAgg                                                                                                        <  2:509259/140‑1 (MQ=255)                               ttGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAgg                                                                                                        <  2:555184/140‑1 (MQ=255)                                      gAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTa                                                                                                 >  1:1091110/1‑140 (MQ=255)                                        aaaGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAAt                                                                                               >  1:270546/1‑140 (MQ=255)                                                      ccATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTa                                                                                 >  2:151874/1‑140 (MQ=255)                                                                gATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGccc                                                                       >  1:355313/1‑140 (MQ=255)                                                                  tGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGccccc                                                                     <  1:990352/140‑1 (MQ=255)                                                                  tGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGccccc                                                                     <  1:234497/140‑1 (MQ=255)                                                                  tGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGccccc                                                                     <  1:842998/140‑1 (MQ=255)                                                                   ggCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCcg                                                                    >  2:872343/1‑140 (MQ=255)                                                                   ggCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCcg                                                                    >  2:53689/1‑140 (MQ=255)                                                                    gCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCcgc                                                                   >  1:880895/1‑140 (MQ=255)                                                                     cAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCcgcg                                                                  <  2:270546/140‑1 (MQ=255)                                                                     cAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCcgcg                                                                  >  2:252309/1‑140 (MQ=255)                                                                     cAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCcgcg                                                                  <  2:547685/140‑1 (MQ=255)                                                                       gagGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTc                                                                >  1:830498/1‑140 (MQ=255)                                                                        agGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCt                                                               >  1:1115632/1‑140 (MQ=255)                                                                        agGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCt                                                               >  1:154643/1‑140 (MQ=255)                                                                          gTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTg                                                             <  1:151874/140‑1 (MQ=255)                                                                             ataaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATc                                                          >  2:244424/1‑140 (MQ=255)                                                                              taaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCa                                                         <  1:561159/140‑1 (MQ=255)                                                                              taaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCa                                                         <  1:252309/140‑1 (MQ=255)                                                                              taaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCa                                                         <  2:211024/140‑1 (MQ=255)                                                                              taaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCAc                                                        <  2:1091110/140‑1 (MQ=255)                                                                               aaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCAc                                                        <  2:880895/140‑1 (MQ=255)                                                                               aaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCAc                                                        <  2:741115/140‑1 (MQ=255)                                                                               aaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCAc                                                        <  1:53689/140‑1 (MQ=255)                                                                                  aTTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGcccc                                                                      >  1:769655/1‑123 (MQ=255)                                                                                  aTTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGcccc                                                                      <  2:769655/123‑1 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGAt                                                           >  2:89487/1‑132 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGAt                                                           >  2:1123280/1‑132 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGAt                                                           <  1:89487/132‑1 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGAt                                                           <  1:1123280/132‑1 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTg                                                   >  2:459053/1‑140 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTg                                                   >  1:2070/1‑140 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTg                                                   >  1:936940/1‑140 (MQ=255)                                                                                    ttCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTg                                                   >  1:914693/1‑140 (MQ=255)                                                                                                   tatCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGTTCACCTCTGCATACCATCCGAAGc                                    >  1:252133/1‑140 (MQ=255)                                                                                                     tCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCtt                                  >  2:345851/1‑140 (MQ=255)                                                                                                       ccTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCtttt                                >  2:378063/1‑140 (MQ=255)                                                                                                        cTTTATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTc                               >  2:739823/1‑140 (MQ=255)                                                                                                           tATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTg                            >  1:908154/1‑140 (MQ=255)                                                                                                           tATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTg                            >  1:628722/1‑140 (MQ=255)                                                                                                           tATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTg                            >  1:545134/1‑140 (MQ=255)                                                                                                           tATGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTCTCTTg                            >  2:916817/1‑140 (MQ=255)                                                                                                            aTGGTGCAAAGAAAGAATTAACGCATCGCACCCACACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGc                           <  2:830498/140‑1 (MQ=255)                                                                                                             tGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCtt                             >  2:223780/1‑137 (MQ=255)                                                                                                             tGGTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCtt                             <  1:223780/137‑1 (MQ=255)                                                                                                               gTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCgtg                        >  2:435923/1‑140 (MQ=255)                                                                                                               gTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCgtg                        >  2:840543/1‑140 (MQ=255)                                                                                                               gTGCAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCgtg                        <  1:840543/140‑1 (MQ=255)                                                                                                                  cAAAGAAAGAATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTc                     >  2:892325/1‑140 (MQ=255)                                                                                                                    aagaaagaATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCtt                             <  1:271582/130‑1 (MQ=255)                                                                                                                    aagaaagaATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCtt                             >  2:271582/1‑130 (MQ=255)                                                                                                                    aagaaagaATTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCtt                   >  1:429408/1‑140 (MQ=255)                                                                                                                     agaaagaaTTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCg                          >  2:539267/1‑132 (MQ=255)                                                                                                                     agaaagaaTTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCg                          <  1:539267/132‑1 (MQ=255)                                                                                                                     agaaagaaTTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCCtg                  >  2:1070325/1‑140 (MQ=255)                                                                                                                          gaaTTAACGCATCGCACCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAgg             <  2:908154/140‑1 (MQ=255)                                                                                                                                cagcatcgccccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcct       <  1:192277/138‑1 (MQ=255)                                                                                                                                 cgcatcgcacCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt      >  1:1100267/1‑140 (MQ=255)                                                                                                                                 cgcatcgcacCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt      >  1:1050565/1‑140 (MQ=255)                                                                                                                                   catcgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCg    <  2:936940/140‑1 (MQ=255)                                                                                                                                    atcgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGc   <  2:355313/140‑1 (MQ=255)                                                                                                                                     tcgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcct       <  2:554032/135‑1 (MQ=255)                                                                                                                                     tcgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcct       >  1:554032/1‑135 (MQ=255)                                                                                                                                     tcgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGcc  <  1:345851/140‑1 (MQ=255)                                                                                                                                      cgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt      >  2:71782/1‑135 (MQ=255)                                                                                                                                      cgcaccCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt      <  1:71782/135‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TTGTTCCACCGTTAATGATTCAGCCAATGATTGTGCAGAAAAGGCTAACGGTGCCATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC  >  NC_000913/3902125‑3902396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑