Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,998,395	C→T	P138L (CCG→CTG)	uvrD →	ssDNA translocase and dsDNA helicase ‑ DNA helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,998,395	0	C	T	100.0%	334.6 / NA	93	P138L (CCG→CTG)	uvrD	ssDNA translocase and dsDNA helicase ‑ DNA helicase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/45);  total (48/45)

CTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGA  >  NC_000913/3998259‑3998518                                                                                                                                         |                                                                                                                            cTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGgctgcc                                                                                                                          >  2:129395/1‑140 (MQ=255)   ggCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCtgccgc                                                                                                                        >  2:951022/1‑140 (MQ=255)         ccGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCggcagg                                                                                                                  <  1:840060/140‑1 (MQ=255)         ccGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCggcagg                                                                                                                  <  2:810900/140‑1 (MQ=255)          cGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCggcaggc                                                                                                                 >  1:298890/1‑140 (MQ=255)            tttGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCaa                                                                                                               >  1:749580/1‑140 (MQ=255)              tgctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCggcagg                                                                                                                  <  1:807257/135‑1 (MQ=255)              tgctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCggcagg                                                                                                                  >  2:807257/1‑135 (MQ=255)              tgctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAAtg                                                                                                             >  1:35103/1‑140 (MQ=255)               gctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAAtgt                                                                                                            <  1:129395/140‑1 (MQ=255)                ctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTACAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGgctgcc                                                                                                                          <  1:803407/125‑1 (MQ=255)                ctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTACAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGgctgcc                                                                                                                          >  2:803407/1‑125 (MQ=255)                 tgcgtgcgCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTgg                                                                                                          <  2:520439/140‑1 (MQ=255)                   cgtgcgCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTa                                                                                                        <  1:795487/140‑1 (MQ=255)                    gtgcgCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAAtgt                                                                                                            <  2:464919/135‑1 (MQ=255)                    gtgcgCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAAtgt                                                                                                            >  1:464919/1‑135 (MQ=255)                      gcgcACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACAt                                                                                                     >  2:599955/1‑140 (MQ=255)                       cgcACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATc                                                                                                    <  2:487151/140‑1 (MQ=255)                          aCCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAAc                                                                                                 <  2:266792/140‑1 (MQ=255)                             atatGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGc                                                                                              >  2:724896/1‑140 (MQ=255)                                  gACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGaa                                                                                         <  1:369387/140‑1 (MQ=255)                                    cGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAg                                                                                       >  1:882535/1‑140 (MQ=255)                                    cGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAg                                                                                       >  2:91237/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  1:45871/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  1:101741/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  1:641780/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  2:382722/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  2:926281/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  1:171150/1‑140 (MQ=255)                                      ccAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGAt                                                                                     >  2:15881/1‑140 (MQ=255)                                         aTCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGaa                                                                                  <  1:382722/140‑1 (MQ=255)                                         aTCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGaa                                                                                  >  1:30099/1‑140 (MQ=255)                                          tCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAg                                                                                 >  2:784748/1‑140 (MQ=255)                                                  aGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGt                                                                         <  1:784748/140‑1 (MQ=255)                                                  aGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGt                                                                         <  2:256679/140‑1 (MQ=255)                                                    gATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTcc                                                                       <  1:599955/140‑1 (MQ=255)                                                    gATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTcc                                                                       <  1:937083/140‑1 (MQ=255)                                                      tttCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGc                                                                     >  1:102439/1‑140 (MQ=255)                                                       ttCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGca                                                                    <  1:724896/140‑1 (MQ=255)                                                         ccAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGcatc                                                                  <  2:35103/140‑1 (MQ=255)                                                         ccAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGcatc                                                                  <  2:978545/140‑1 (MQ=255)                                                          cAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGcatca                                                                 <  1:926281/140‑1 (MQ=255)                                                              tCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATAtt                                                             <  2:171150/140‑1 (MQ=255)                                                               ccTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTc                                                            <  2:608131/140‑1 (MQ=255)                                                                 tCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGcatc                                                                  <  1:57913/132‑1 (MQ=255)                                                                 tCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGcatc                                                                  >  2:57913/1‑132 (MQ=255)                                                                  cGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATAtt                                                             <  1:1132355/136‑1 (MQ=255)                                                                  cGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATAtt                                                             <  1:501119/136‑1 (MQ=255)                                                                  cGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATAtt                                                             >  2:1132355/1‑136 (MQ=255)                                                                  cGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATAtt                                                             >  2:501119/1‑136 (MQ=255)                                                                  cGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCaaa                                                         >  1:833725/1‑140 (MQ=255)                                                                        tGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACg                                                   >  1:1027750/1‑140 (MQ=255)                                                                        tGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACg                                                   >  1:626537/1‑140 (MQ=255)                                                                            gACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTaa                                               <  2:833725/140‑1 (MQ=255)                                                                             aCCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAAt                                              >  1:73718/1‑140 (MQ=255)                                                                             aCCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAAt                                              >  1:442215/1‑140 (MQ=255)                                                                             aCCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAAt                                              >  2:859446/1‑140 (MQ=255)                                                                             aCCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAAt                                              >  1:226200/1‑140 (MQ=255)                                                                              ccAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATc                                             <  2:226200/140‑1 (MQ=255)                                                                                 gCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCgg                                          <  2:30099/140‑1 (MQ=255)                                                                                  cTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGt                                         >  1:812944/1‑140 (MQ=255)                                                                                    aCGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAAACCGGTgg                                       >  1:209373/1‑140 (MQ=255)                                                                                      gCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAg                                     <  1:717174/140‑1 (MQ=255)                                                                                      gCCTGCTTAAGCGTCTAATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAg                                     <  2:73718/140‑1 (MQ=255)                                                                                       ccTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGc                                    <  1:74592/140‑1 (MQ=255)                                                                                       ccTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGc                                    >  2:318430/1‑140 (MQ=255)                                                                                          gCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGa                                 >  2:788775/1‑140 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAg                                     >  2:719853/1‑135 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAg                                     <  1:719853/135‑1 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAc                                >  1:916411/1‑140 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAc                                <  1:829087/140‑1 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAc                                >  1:872003/1‑140 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAc                                >  1:966909/1‑140 (MQ=255)                                                                                           cTTAAGCGTCTGATCAAAGCCATGAACCTCGACAAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAc                                <  2:1027750/140‑1 (MQ=255)                                                                                            ttAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAg                                     <  1:94018/134‑1 (MQ=255)                                                                                            ttAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAg                                     >  2:94018/1‑134 (MQ=255)                                                                                            ttAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAcc                               <  2:749580/140‑1 (MQ=255)                                                                                            ttAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGAcc                               >  1:665843/1‑140 (MQ=255)                                                                                                gCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGc                           <  2:872003/140‑1 (MQ=255)                                                                                                gCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGc                           >  2:584690/1‑140 (MQ=255)                                                                                                 cGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCa                          <  2:641780/140‑1 (MQ=255)                                                                                                    cTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGaa                       <  1:788775/140‑1 (MQ=255)                                                                                                     tGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAg                      >  2:1004710/1‑140 (MQ=255)                                                                                                     tGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAg                      >  2:733723/1‑140 (MQ=255)                                                                                                          aaaGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTa                 <  2:59149/140‑1 (MQ=255)                                                                                                            aGCCATGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATc               >  2:794389/1‑140 (MQ=255)                                                                                                                aTGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGc           <  2:557617/140‑1 (MQ=255)                                                                                                                aTGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGc           <  1:733723/140‑1 (MQ=255)                                                                                                                aTGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGc           <  2:916411/140‑1 (MQ=255)                                                                                                                 tGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGGAGACCTGGCAGAAGGTGTATCAGGCg          >  1:106254/1‑140 (MQ=255)                                                                                                                 tGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCa                          <  1:21957/124‑1 (MQ=255)                                                                                                                 tGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCa                          >  2:21957/1‑124 (MQ=255)                                                                                                                 tGAACCTCGACGAGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCg          >  2:797139/1‑140 (MQ=255)                                                                                                                        cgacgaGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAgg   <  2:882535/140‑1 (MQ=255)                                                                                                                        cgacgaGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAgg   <  2:966909/140‑1 (MQ=255)                                                                                                                         gacgaGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGa  >  1:274803/1‑140 (MQ=255)                                                                                                                         gacgaGAAGCAGTGGCTGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGa  >  2:1139329/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                            CTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGA  >  NC_000913/3998259‑3998518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑