Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,001,107	T→C	intergenic (‑51/‑319)	yigE ← / → corA	DUF2233 domain‑containing protein YigE/Ni(2(+))/Co(2(+))/Mg(2(+)) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,001,107	0	T	C	100.0%	356.7 / NA	97	intergenic (‑51/‑319)	yigE/corA	DUF2233 domain‑containing protein YigE/Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/56);  total (41/56)

AAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAGTAGATACT  >  NC_000913/4000979‑4001239                                                                                                                                 |                                                                                                                                     aaGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAgcg                                                                                                                           <  2:379716/140‑1 (MQ=255)   gTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGcgcg                                                                                                                         <  2:1094081/140‑1 (MQ=255)   gTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGcgcg                                                                                                                         <  2:439376/140‑1 (MQ=255)   gTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGcgcg                                                                                                                         <  1:615544/140‑1 (MQ=255)   gTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGcgcg                                                                                                                         <  1:558577/140‑1 (MQ=255)   gTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGcgcg                                                                                                                         <  2:1129075/140‑1 (MQ=255)    tAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGa                                                                                                                        <  1:247361/140‑1 (MQ=255)      aGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAg                                                                                                                      >  1:678445/1‑140 (MQ=255)         tGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTc                                                                                                                   <  2:785667/140‑1 (MQ=255)          gAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCa                                                                                                                  >  1:1151790/1‑140 (MQ=255)          gAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCa                                                                                                                  >  2:81563/1‑140 (MQ=255)             ggCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACt                                                                                                               >  2:869992/1‑140 (MQ=255)              gCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGa                                                                                                                        <  2:23746/130‑1 (MQ=255)              gCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGa                                                                                                                        >  1:23746/1‑130 (MQ=255)              gCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCa                                                                                                                  <  1:825145/136‑1 (MQ=255)              gCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCa                                                                                                                  >  2:825145/1‑136 (MQ=255)              gCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTa                                                                                                              <  2:112639/140‑1 (MQ=255)                 agaAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTg                                                                                                           >  2:617673/1‑140 (MQ=255)                  gaAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGt                                                                                                          >  1:840980/1‑140 (MQ=255)                   aaaaaTCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTa                                                                                                              >  2:512086/1‑135 (MQ=255)                   aaaaaTCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTa                                                                                                              <  1:512086/135‑1 (MQ=255)                   aaaaaTCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGtt                                                                                                         <  1:923704/140‑1 (MQ=255)                      aaTCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCtg                                                                                                      <  2:254000/140‑1 (MQ=255)                      aaTCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCtg                                                                                                      <  2:587215/140‑1 (MQ=255)                        tCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTTTAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAg                                                                                                                      >  1:556206/1‑122 (MQ=255)                        tCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTTTAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAg                                                                                                                      <  2:556206/122‑1 (MQ=255)                        tCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCtgtg                                                                                                    <  1:5194/140‑1 (MQ=255)                        tCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCtgtg                                                                                                    <  2:932185/140‑1 (MQ=255)                                               aTTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGc                                                                             <  1:43282/140‑1 (MQ=255)                                                  ccTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCaaa                                                                          >  2:659037/1‑140 (MQ=255)                                                      tACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAAttt                                                                      >  1:899036/1‑140 (MQ=255)                                                      tACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAAttt                                                                      >  2:884063/1‑140 (MQ=255)                                                      tACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAAttt                                                                      >  2:77423/1‑140 (MQ=255)                                                        ccAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTg                                                                    <  1:81563/140‑1 (MQ=255)                                                        ccAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTg                                                                    <  1:113111/140‑1 (MQ=255)                                                          aaTGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCt                                                                  <  2:678445/140‑1 (MQ=255)                                                           aTGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTg                                                                    >  2:1124041/1‑137 (MQ=255)                                                           aTGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTg                                                                    <  1:1124041/137‑1 (MQ=255)                                                           aTGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTg                                                                 >  1:538873/1‑140 (MQ=255)                                                            tGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTgg                                                                >  1:575288/1‑140 (MQ=255)                                                            tGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTAATGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTgg                                                                >  1:765366/1‑140 (MQ=255)                                                                   ttGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACa                                                         >  2:1077346/2‑140 (MQ=255)                                                                   cTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACa                                                         >  1:287766/1‑140 (MQ=255)                                                                       tGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATg                                                       <  2:50876/138‑1 (MQ=255)                                                                       tGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATg                                                       >  1:964516/1‑138 (MQ=255)                                                                       tGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATg                                                       >  1:50876/1‑138 (MQ=255)                                                                       tGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATg                                                       <  2:964516/138‑1 (MQ=255)                                                                        gcgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGTGTAACATgcgc                                                    >  2:220577/1‑140 (MQ=255)                                                                        gcgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATgcgc                                                    >  2:556790/1‑140 (MQ=255)                                                                        gcgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATgcgc                                                    >  2:968795/1‑140 (MQ=255)                                                                        gcgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATgcgc                                                    >  2:178225/1‑140 (MQ=255)                                                                        gcgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATgcgc                                                    >  2:832360/1‑140 (MQ=255)                                                                        gcgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATgcgc                                                    >  1:470897/1‑140 (MQ=255)                                                                         cgcCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATgcgcg                                                   <  2:840980/140‑1 (MQ=255)                                                                           ccATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCa                                                 <  1:77423/140‑1 (MQ=255)                                                                             aTTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACg                                               <  1:556790/140‑1 (MQ=255)                                                                               tataGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  1:968795/140‑1 (MQ=255)                                                                               tataGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  2:575288/140‑1 (MQ=255)                                                                               tataGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  1:659037/140‑1 (MQ=255)                                                                               tataGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  1:869992/140‑1 (MQ=255)                                                                               tataGGTCCTGGATGTAGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  2:1038454/140‑1 (MQ=255)                                                                                    gTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCActc                                        >  1:357624/1‑140 (MQ=255)                                                                                      ccTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             >  1:814264/1‑133 (MQ=255)                                                                                      ccTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  2:814264/133‑1 (MQ=255)                                                                                      ccTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTa                                      >  2:84397/1‑140 (MQ=255)                                                                                      ccTGGATGTGGGATTTTTTTATCC‑GTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTaa                                     >  2:632702/1‑140 (MQ=255)                                                                                        tGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCa                                                 >  1:66655/1‑127 (MQ=255)                                                                                        tGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCa                                                 <  2:66655/127‑1 (MQ=255)                                                                                        tGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAag                                    >  1:80090/1‑140 (MQ=255)                                                                                        tGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAag                                    >  1:137422/1‑140 (MQ=255)                                                                                        tGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAag                                    >  2:954766/1‑140 (MQ=255)                                                                                        tGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAag                                    >  2:967880/1‑140 (MQ=255)                                                                                         ggATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAaga                                   >  2:659081/1‑140 (MQ=255)                                                                                              tgGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             <  1:18271/125‑1 (MQ=255)                                                                                              tgGGATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt                                             >  2:18271/1‑125 (MQ=255)                                                                                               gggATTTTTTTATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACAt                             <  2:1052233/140‑1 (MQ=255)                                                                                                     tttttATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCt                       <  1:884063/140‑1 (MQ=255)                                                                                                     tttttATCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCt                       <  2:871978/140‑1 (MQ=255)                                                                                                          tatccgTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac                  <  1:632702/135‑1 (MQ=255)                                                                                                          aTCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGTGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac                  <  1:220577/140‑1 (MQ=255)                                                                                                          aTCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac                  <  2:683454/140‑1 (MQ=255)                                                                                                          aTCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac                  <  2:1080954/140‑1 (MQ=255)                                                                                                          aTCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac                  <  1:725138/140‑1 (MQ=255)                                                                                                          aTCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac                  <  2:137422/140‑1 (MQ=255)                                                                                                          aTCCTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCCTGGac                  <  1:1077346/140‑1 (MQ=255)                                                                                                             cTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGacaca               <  2:724268/140‑1 (MQ=255)                                                                                                             cTGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGacaca               <  1:146560/140‑1 (MQ=255)                                                                                                              tGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGacacac              <  2:538873/140‑1 (MQ=255)                                                                                                              tGTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGacacac              <  1:745365/140‑1 (MQ=255)                                                                                                               gTTAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACAcc             <  1:178225/140‑1 (MQ=255)                                                                                                                ttAGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACAccc            <  2:556607/140‑1 (MQ=255)                                                                                                                  aGCGACCTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAg          <  2:357624/140‑1 (MQ=255)                                                                                                                       ccTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAGTAGAt     <  2:287766/140‑1 (MQ=255)                                                                                                                        cTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAGTAGATa    <  1:512705/140‑1 (MQ=255)                                                                                                                        cTTGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAGTAGATa    >  1:433139/1‑140 (MQ=255)                                                                                                                          tGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACAcc             <  2:353763/129‑1 (MQ=255)                                                                                                                          tGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACAcc             >  1:353763/1‑129 (MQ=255)                                                                                                                          tGACGAGCACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAGTAGATACt  >  1:1073162/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     AAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACACCCAGTAGATACT  >  NC_000913/4000979‑4001239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑