Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,134,855	A→G	T341A (ACC→GCC)	katG →	hydroperoxidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,134,855	0	A	G	100.0%	149.3 / NA	41	T341A (ACC→GCC)	katG	hydroperoxidase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

GGCGAGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGACCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAG  >  NC_000913/4134725‑4134989                                                                                                                                   |                                                                                                                                       ggCGAGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGcc                                                                                                                               >  2:1017575/1‑140 (MQ=255) ggCGAGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGcc                                                                                                                               >  1:757230/1‑140 (MQ=255) ggCGAGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGc                                                                                                                                <  2:826497/139‑1 (MQ=255) ggCGAGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGc                                                                                                                                >  1:826497/1‑139 (MQ=255)     aGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGc                                                                                                                           <  1:759457/140‑1 (MQ=255)     aGCACTTACGGCAGCAGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGc                                                                                                                           <  2:234025/140‑1 (MQ=255)            aCGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCa                                                                                                                    <  1:154631/140‑1 (MQ=255)                    ggcgTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGtt                                                                                                            >  1:163984/1‑140 (MQ=255)                    ggcgTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGtt                                                                                                            >  2:449033/1‑140 (MQ=255)                    ggcgTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGtt                                                                                                            <  1:335968/140‑1 (MQ=255)                      cgTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCg                                                                                                          <  2:757230/140‑1 (MQ=255)                        ttGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGaa                                                                                                        <  1:449033/140‑1 (MQ=255)                                gATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGa                                                                                                <  2:1149499/140‑1 (MQ=255)                                 aTGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGAc                                                                                               <  2:163984/140‑1 (MQ=255)                                            tctggtctggAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAAt                                                                                    <  2:385111/140‑1 (MQ=255)                                               ggtctggAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTAt                                                                                 >  2:610182/1‑140 (MQ=255)                                                gtctggAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATc                                                                                <  1:1017575/140‑1 (MQ=255)                                                          agtCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGt                                                                      >  1:87785/1‑140 (MQ=255)                                                          agtCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGt                                                                      >  2:699959/1‑140 (MQ=255)                                                           gtCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGtt                                                                     >  2:159353/1‑140 (MQ=255)                                                            tCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGa                                                                           <  2:614036/133‑1 (MQ=255)                                                            tCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGa                                                                           >  1:614036/1‑133 (MQ=255)                                                                           cGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGaaa                                                     >  2:882241/1‑140 (MQ=255)                                                                           cGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGaaa                                                     >  2:936134/1‑140 (MQ=255)                                                                                       gCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACa                                         <  1:235960/140‑1 (MQ=255)                                                                                               ttcttcGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGt                                 >  1:1139791/1‑140 (MQ=255)                                                                                                tcttcGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTg                                >  1:821837/1‑140 (MQ=255)                                                                                                  ttcGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTgac                              <  1:699959/140‑1 (MQ=255)                                                                                                  ttcGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTgac                              <  2:865748/140‑1 (MQ=255)                                                                                                   tcGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTg                                >  2:570037/1‑137 (MQ=255)                                                                                                   tcGAGAACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTg                                <  1:570037/137‑1 (MQ=255)                                                                                                     gagaACCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTgaccga                           >  2:495274/1‑140 (MQ=255)                                                                                                        aaCCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCt                        >  2:460245/1‑140 (MQ=255)                                                                                                         aCCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTg                       <  2:519266/140‑1 (MQ=255)                                                                                                         aCCTGTTCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTg                       <  1:610182/140‑1 (MQ=255)                                                                                                              ttCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCt                  >  2:393338/1‑140 (MQ=255)                                                                                                               tCAAGTATGAGTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTg                 <  2:87785/140‑1 (MQ=255)                                                                                                                         gTGGGTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATc       <  1:460245/140‑1 (MQ=255)                                                                                                                             gTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGa   >  2:645943/1‑140 (MQ=255)                                                                                                                             gTACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGa   >  2:666716/1‑140 (MQ=255)                                                                                                                              tACAGGCCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAg  >  2:179062/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       GGCGAGCACTTACGGCAGCGGCGTTGGCGCAGATGCCATTACCTCTGGTCTGGAAGTAGTCTGGACCCAGACGCCGACCCAGTGGAGCAACTATTTCTTCGAGAACCTGTTCAAGTATGAGTGGGTACAGACCCGCAGCCCGGCTGGCGCAATCCAGTTCGAAGCGGTAGACGCACCGGAAATTATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAG  >  NC_000913/4134725‑4134989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑