Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,168,396	A→G	noncoding (25/76 nt)	gltT →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,168,396	0	A	G	100.0%	62.0 / NA	21	noncoding (25/76 nt)	gltT	tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

TCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAA  >  NC_000913/4168343‑4168530                                                      |                                                                                                                                       tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                                                  <  1:1048465/140‑1 (MQ=17) tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                                                  >  1:56613/1‑140 (MQ=17)      cAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtct                                             <  1:238462/140‑1 (MQ=18)       aaCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGGAACAGGGGTTCGAATCCCCCAGGGGACGCCCCTTTCTGGTTTGTGAGGGAAAGTCGCCCACCTTAATAtctc                                            >  2:99718/1‑140 (MQ=11)         cgcgTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAGGGGACGCCACTTGCTGGTTTTTGAGGGAAAGTCGCCGACCTTAATATCTCaa                                          >  2:892142/1‑140 (MQ=11)               aTAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCa                                    <  1:425454/140‑1 (MQ=25)               aTAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCa                                    >  1:315351/1‑140 (MQ=21)               aTAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCa                                    >  1:38108/1‑140 (MQ=19)                   gCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCtt                                <  1:550368/140‑1 (MQ=255)                       ttttCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCggg                            <  1:962050/140‑1 (MQ=255)                         ttCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTg                          <  2:426286/140‑1 (MQ=255)                         ttCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTg                          <  2:70067/140‑1 (MQ=255)                         ttCGTGTCCCCTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTg                          <  2:777042/140‑1 (MQ=255)                                 cccTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTga                  <  1:717211/140‑1 (MQ=255)                                 cccTTCGTCTAGAGGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTga                  <  2:56613/140‑1 (MQ=38)                                            gagGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGctc       >  2:409439/1‑140 (MQ=37)                                            gagGCCCAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGctc       >  2:650087/1‑140 (MQ=37)                                                cccAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTa   <  1:328954/140‑1 (MQ=38)                                                 ccAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGct        >  1:100376/1‑134 (MQ=37)                                                 ccAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGct        <  2:100376/134‑1 (MQ=38)                                                 ccAGGGCACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGGGATGTTTGAGATATTTGCTCTTTaa  >  1:708507/1‑140 (MQ=255)                                                      |                                                                                                                                       TCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAA  >  NC_000913/4168343‑4168530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑