Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,300,353	C→T	A226A (GCG→GCA)	mdtP ←	putative multidrug efflux pump outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,300,353	0	C	T	100.0%	206.9 / NA	59	A226A (GCG→GCA)	mdtP	putative multidrug efflux pump outer membrane channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/41);  total (18/41)

GGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCATACTGTAATAAAGCTGCGCTACGCCCG  >  NC_000913/4300218‑4300468                                                                                                                                        |                                                                                                                    ggCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCa                                                                                                                 <  1:912854/140‑1 (MQ=255)  gCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgtgc                                                                                                                   >  1:638437/1‑137 (MQ=255)  gCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCAc                                                                                                                >  2:20001/1‑140 (MQ=255)  gCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCAc                                                                                                                >  2:254643/1‑140 (MQ=255)       cgcgCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTAc                                                                                                           >  1:456686/1‑140 (MQ=255)       cgcgCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTAc                                                                                                           >  2:915691/1‑140 (MQ=255)       cgcgCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTAc                                                                                                           >  1:1048598/1‑140 (MQ=255)       cgcgCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGCGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTAc                                                                                                           >  2:536269/1‑140 (MQ=255)        gcgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTAct                                                                                                          <  1:915691/140‑1 (MQ=255)            caatcaatGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTgg                                                                                                      <  1:168681/140‑1 (MQ=255)              atcaatGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTg                                                                                                    <  1:640167/140‑1 (MQ=255)                    gCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCttt                                                                                              <  1:837910/140‑1 (MQ=255)                      aCGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCa                                                                                            >  1:239766/1‑140 (MQ=255)                        gCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCAcc                                                                                          >  1:204716/1‑140 (MQ=255)                        gCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCAcc                                                                                          >  2:808638/1‑140 (MQ=255)                          agagATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACcgc                                                                                        >  1:330350/1‑140 (MQ=255)                           gagaTTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACcgcg                                                                                       <  2:605237/140‑1 (MQ=255)                           gagaTTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCCTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACcgcg                                                                                       <  2:976008/140‑1 (MQ=255)                               ttCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTaat                                                                                   <  2:50336/140‑1 (MQ=255)                                tctcGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatc                                                                                  >  1:994205/1‑140 (MQ=255)                                 ctcGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTaat                                                                                   <  1:594365/138‑1 (MQ=255)                                 ctcGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTaat                                                                                   >  2:594365/1‑138 (MQ=255)                                   cgcgTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaat                                                                               <  1:20001/140‑1 (MQ=255)                                               aTTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt                                                                   <  2:239766/140‑1 (MQ=255)                                               aTTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt                                                                   <  1:1081359/140‑1 (MQ=255)                                                tttGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt                                                                  <  2:994205/140‑1 (MQ=255)                                                tttGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt                                                                  <  2:691750/140‑1 (MQ=255)                                                tttGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt                                                                  <  1:849660/140‑1 (MQ=255)                                                  tGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTg                                                                <  1:600117/140‑1 (MQ=255)                                                  tGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTg                                                                <  2:1048598/140‑1 (MQ=255)                                                   gCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGt                                                               <  1:254643/140‑1 (MQ=255)                                                    cccTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGtt                                                              >  1:881481/1‑140 (MQ=255)                                                    cccTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGtt                                                              >  1:709148/1‑140 (MQ=255)                                                      cTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCt                                                            <  2:881481/140‑1 (MQ=255)                                                      cTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCATGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCt                                                            <  1:245800/140‑1 (MQ=255)                                                        ttGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTaa                                                          <  1:967695/140‑1 (MQ=255)                                                         tGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt                                                                   <  2:51889/130‑1 (MQ=255)                                                         tGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt                                                                   >  1:51889/1‑130 (MQ=255)                                                          gACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAGTCAATCACATCGTGAGTTTGTTCTAACa                                                        <  2:330350/140‑1 (MQ=255)                                                              gCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTCGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATc                                                    <  1:890547/140‑1 (MQ=255)                                                                 acaaTTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAg                                                 <  2:379579/140‑1 (MQ=255)                                                                 acaaTTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAg                                                 <  2:456686/140‑1 (MQ=255)                                                                 acaaTTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAg                                                 <  2:536134/140‑1 (MQ=255)                                                                           tATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAg                                       <  1:207556/140‑1 (MQ=255)                                                                           tATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAg                                       <  2:622324/140‑1 (MQ=255)                                                                           tATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAg                                       <  2:204716/140‑1 (MQ=255)                                                                           tATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAAACACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAg                                       <  1:393916/140‑1 (MQ=255)                                                                                aCCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGc                                  <  2:567915/140‑1 (MQ=255)                                                                                     cAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCa                             <  2:929896/140‑1 (MQ=255)                                                                                     cAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCa                             <  1:132456/140‑1 (MQ=255)                                                                                     cAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCa                             <  1:675441/140‑1 (MQ=255)                                                                                     cAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCa                             <  1:808638/140‑1 (MQ=255)                                                                                        aaTCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCATAc                          <  1:626094/140‑1 (MQ=255)                                                                                            tgtgCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAg                                       >  1:986910/1‑123 (MQ=255)                                                                                            tgtgCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAg                                       <  2:986910/123‑1 (MQ=255)                                                                                              tgCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGTTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGc                                  <  1:400374/126‑1 (MQ=255)                                                                                              tgCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGc                                  >  2:400374/1‑126 (MQ=255)                                                                                               gCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCATACTGtaata                   >  2:564009/1‑140 (MQ=255)                                                                                                   gcgcgcCGTGGAAAGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCATACTGTAATAAAGc               <  2:228865/140‑1 (MQ=255)                                                                                                                aGGCACTTGCGCTTCCAGACCGTGTGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCATACTGTAATAAAGCTGCGCTACGCCCg  <  1:564009/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                    GGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAACAGATCGAGCATCTGATAGCTGGCCTGCATACTGTAATAAAGCTGCGCTACGCCCG  >  NC_000913/4300218‑4300468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑