Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,192	C→T	D107D (GAC→GAT)	yaiW →	surface‑exposed outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,192	0	C	T	100.0%	161.0 / NA	48	D107D (GAC→GAT)	yaiW	surface‑exposed outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/24);  total (24/24)

ACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACG  >  NC_000913/398055‑398328                                                                                                                                          |                                                                                                                                         accaccTTTGAAAGTCAGGTGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATcg                                                                                                                                        <  2:1096702/140‑1 (MQ=255)        ttGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCt                                                                                                                                 >  2:681671/1‑140 (MQ=255)         tGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTg                                                                                                                                <  1:96074/140‑1 (MQ=255)           aaaGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGaa                                                                                                                              <  2:450372/140‑1 (MQ=255)           aaaGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGaa                                                                                                                              <  2:457518/140‑1 (MQ=255)                cAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGAt                                                                                                                         >  1:110926/1‑140 (MQ=255)                cAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGAt                                                                                                                         >  2:446037/1‑140 (MQ=255)                cAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGAt                                                                                                                         >  1:465773/1‑140 (MQ=255)                         gCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCTGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTcc                                                                                                                <  2:801827/140‑1 (MQ=255)                         gCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTcc                                                                                                                >  1:68015/1‑140 (MQ=255)                             cGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCt                                                                                                            >  2:742302/1‑140 (MQ=255)                               aCGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCttt                                                                                                          >  2:543191/1‑140 (MQ=255)                                cGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCtttt                                                                                                         <  2:532133/140‑1 (MQ=255)                                cGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCtttt                                                                                                         <  1:1122678/140‑1 (MQ=255)                                cGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCtttt                                                                                                         <  1:543191/140‑1 (MQ=255)                                   tGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTg                                                                                                      >  2:335560/1‑140 (MQ=255)                                               gCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGGTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACcgcg                                                                                          >  2:391641/1‑140 (MQ=255)                                                cTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCgcgc                                                                                         >  2:696577/1‑140 (MQ=255)                                                  cGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTa                                                                                       <  2:110926/140‑1 (MQ=255)                                                     tGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTaaaa                                                                                    >  2:393817/1‑140 (MQ=255)                                                               ggCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGc                                                                          >  1:329496/1‑140 (MQ=255)                                                               ggCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGc                                                                          >  1:773179/1‑140 (MQ=255)                                                                 cgcAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCa                                                                        <  1:967809/140‑1 (MQ=255)                                                                   cagcagGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCaaa                                                                      <  1:681671/140‑1 (MQ=255)                                                                   cagcagGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCaaa                                                                      <  2:68015/140‑1 (MQ=255)                                                                       agGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGc                                                                  <  2:773179/140‑1 (MQ=255)                                                                       agGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGc                                                                  <  1:1086865/140‑1 (MQ=255)                                                                        ggAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCa                                                                 <  1:393817/140‑1 (MQ=255)                                                                         gAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCaa                                                                <  2:329496/140‑1 (MQ=255)                                                                          aGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCaaa                                                               >  2:291898/1‑140 (MQ=255)                                                                                          aGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGt                                               <  1:446037/140‑1 (MQ=255)                                                                                            aTCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTa                                             >  1:390661/1‑140 (MQ=255)                                                                                                  ccGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCg                                       <  2:390661/140‑1 (MQ=255)                                                                                                  ccGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCg                                       >  1:392179/1‑140 (MQ=255)                                                                                                      tCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGc                                   >  1:389399/1‑140 (MQ=255)                                                                                                      tCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGc                                   >  1:920011/1‑140 (MQ=255)                                                                                                       ccGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCg                                  <  2:389399/140‑1 (MQ=255)                                                                                                       ccGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCg                                  <  2:361338/140‑1 (MQ=255)                                                                                                          ggCTTAAGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTAc                               >  2:601466/1‑140 (MQ=255)                                                                                                                aGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGaaaa                         >  2:658488/1‑140 (MQ=255)                                                                                                                aGCAAGATCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGaaaa                         <  1:601466/140‑1 (MQ=255)                                                                                                                      aTCGCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAAtt                   >  1:104441/1‑140 (MQ=255)                                                                                                                         gCCTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAg                >  1:368472/1‑140 (MQ=255)                                                                                                                          ccTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAgc               >  1:537480/1‑140 (MQ=255)                                                                                                                          ccTGGCAAGAGATTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAgc               >  2:1117289/1‑140 (MQ=255)                                                                                                                                     aTTGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGa    <  1:335560/140‑1 (MQ=255)                                                                                                                                       tGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACg  <  1:291898/140‑1 (MQ=255)                                                                                                                                       tGATCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACg  <  2:920011/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACG  >  NC_000913/398055‑398328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑