Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,368,924	A→G	L87L (TTA→TTG)	fxsA →	protein FxsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,368,924	0	A	G	98.4%	199.4 / ‑5.1	61	L87L (TTA→TTG)	fxsA	protein FxsA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (28/32);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGT  >  NC_000913/4368795‑4369056                                                                                                                                  |                                                                                                                                     gTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCtttt                                                                                                                            >  2:981480/1‑140 (MQ=255) gTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCtttt                                                                                                                            >  1:274828/1‑140 (MQ=255)       ggTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCg                                                                                                                      <  1:348885/140‑1 (MQ=255)       ggTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCg                                                                                                                      <  2:1090429/140‑1 (MQ=255)        gTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGa                                                                                                                     >  1:948518/1‑140 (MQ=255)        gTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGa                                                                                                                     >  1:902833/1‑140 (MQ=255)        gTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGa                                                                                                                     >  1:683787/1‑140 (MQ=255)          aCGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTTTTTGCGGGCTTTTTTATCggcc                                                                                                                   >  2:655515/1‑137 (MQ=255)           cGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGGGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCCACtt                                                                                                                  >  1:765078/1‑140 (MQ=255)            gTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTc                                                                                                                 >  1:1086839/1‑140 (MQ=255)              aaCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCggg                                                                                                                                 >  1:717971/1‑122 (MQ=255)              aaCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCggg                                                                                                                                 <  2:717971/122‑1 (MQ=255)              aaCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCt                                                                                                               <  1:871172/140‑1 (MQ=255)              aaCAAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGGGAAAACCCAGCGGCGGGGATGATTAAAAGTGTTTTGCCGATCATTGCTGGGTTTCTGCTTTTTTTTCCGGGGTTTTTTTCCGAcctcct                                                                                                               >  2:295628/1‑140 (MQ=255)               aCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTc                                                                                                              <  2:253772/140‑1 (MQ=255)                ccAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCg                                                                                                             >  1:65773/1‑140 (MQ=255)                ccAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCg                                                                                                             <  1:574952/140‑1 (MQ=255)                  aGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGGGATGATTAAAAATGTTTTGCTGATCATTTGTGGGTTTCTTCTTTTTTTTCCGGGCTTTTTTTCCGGCTTcccccgg                                                                                                           >  2:281816/1‑135 (MQ=255)                   gggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGGGATGATTAAAAAGGTTTCGCTGATCATTGCTGGGTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCgggc                                                                                                          >  2:553642/1‑138 (MQ=255)                   gggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtc                                                                                                          <  2:609631/140‑1 (MQ=255)                     gCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtctt                                                                                                        <  2:948518/140‑1 (MQ=255)                      cTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtcttc                                                                                                       <  1:655515/140‑1 (MQ=255)                        ttAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCg                                                                                                             <  2:725064/132‑1 (MQ=255)                        ttAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCg                                                                                                             >  1:725064/1‑132 (MQ=255)                        ttAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTa                                                                                                     >  1:1055745/1‑140 (MQ=255)                         tAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTAc                                                                                                    >  2:501465/1‑140 (MQ=255)                         tAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTAc                                                                                                    <  1:336225/140‑1 (MQ=255)                              aTTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAAAGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTAACGACCTCCTCGGGCTTCTACttttt                                                                                               >  2:1052802/1‑139 (MQ=255)                                    tGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTgccc                                                                                         >  2:906499/1‑139 (MQ=255)                                     gCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTgccgc                                                                                        <  1:1152579/140‑1 (MQ=255)                                     gCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTgccgc                                                                                        <  2:1055745/140‑1 (MQ=255)                                             agcaAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCaa                                                                                <  2:1104676/140‑1 (MQ=255)                                                 aaaaaTGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGc                                                                            >  2:699005/1‑140 (MQ=255)                                                    aaTGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTCTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATc                                                                         <  2:783524/140‑1 (MQ=255)                                                     aTGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCt                                                                        <  2:1086839/140‑1 (MQ=255)                                                     aTGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCt                                                                        <  1:981480/140‑1 (MQ=255)                                                       ggcggcGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGa                                                                      <  1:699005/140‑1 (MQ=255)                                                       ggcggcGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGa                                                                      <  1:437842/140‑1 (MQ=255)                                                         cggcggGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACa                                                                    <  1:281816/140‑1 (MQ=255)                                                              ggTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATc                                                                         >  1:248604/1‑130 (MQ=255)                                                              ggTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATc                                                                         <  2:248604/130‑1 (MQ=255)                                                                   aaaCCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGa                                                          <  1:975826/140‑1 (MQ=255)                                                                         aGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGc                                                    >  2:319858/1‑140 (MQ=255)                                                                          gcggcgGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCa                                                   >  1:68682/1‑140 (MQ=255)                                                                           cggcggAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCAt                                                  <  1:501465/140‑1 (MQ=255)                                                                               ggAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTgc                                              <  1:319858/140‑1 (MQ=255)                                                                                gagaTGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTgcg                                             <  1:821024/140‑1 (MQ=255)                                                                                gagaTGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTgcg                                             >  2:699588/1‑140 (MQ=255)                                                                                     gatTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCtttt                                        <  1:295628/140‑1 (MQ=255)                                                                                          aaaGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGc                                   >  1:1123492/1‑140 (MQ=255)                                                                                                   cGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTggc                          >  1:81166/1‑140 (MQ=255)                                                                                                   cGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTggc                          <  2:683787/140‑1 (MQ=255)                                                                                                      tGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTggcggc                       <  2:81166/140‑1 (MQ=255)                                                                                                       gATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGgcggcg                      <  1:1052802/140‑1 (MQ=255)                                                                                                             tGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTa                >  2:1130337/1‑140 (MQ=255)                                                                                                             tGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTa                >  2:907755/1‑140 (MQ=255)                                                                                                             tGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTa                <  2:1123492/140‑1 (MQ=255)                                                                                                                    ttGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg         >  2:627457/1‑140 (MQ=255)                                                                                                                    ttGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg         >  1:1015114/1‑140 (MQ=255)                                                                                                                       ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg         <  1:734042/137‑1 (MQ=255)                                                                                                                       ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg         >  2:734042/1‑137 (MQ=255)                                                                                                                       ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc      >  1:900799/1‑140 (MQ=255)                                                                                                                       ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc      >  1:136263/1‑140 (MQ=255)                                                                                                                       ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc      >  2:133703/1‑140 (MQ=255)                                                                                                                           ttttATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGt  <  2:68682/140‑1 (MQ=255)                                                                                                                           ttttATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGt  <  1:907755/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     GTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGT  >  NC_000913/4368795‑4369056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑