Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,417,594	A→G	T215A (ACG→GCG)	yjfP →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,417,594	0	A	G	100.0%	159.1 / NA	44	T215A (ACG→GCG)	yjfP	carboxylesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

GCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATGCTGACCCCTTGCGCT  >  NC_000913/4417459‑4417728                                                                                                                                        |                                                                                                                                       gcgcCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAggcggg                                                                                                                                    <  1:407276/140‑1 (MQ=255) gcgcCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAggcggg                                                                                                                                    <  1:214546/140‑1 (MQ=255)               gTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGc                                                                                                                      >  2:459547/1‑140 (MQ=255)                  ggAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTaa                                                                                                                   >  1:346699/1‑140 (MQ=255)                   gAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAAc                                                                                                                  <  2:248790/140‑1 (MQ=255)                   gAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAAc                                                                                                                  >  2:626031/1‑140 (MQ=255)                        gACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGtt                                                                                                             >  2:536713/1‑140 (MQ=255)                                 ccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAAc                                                                                                                  >  2:264360/1‑126 (MQ=255)                                 ccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAAc                                                                                                                  <  1:264360/126‑1 (MQ=255)                                    ggAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAg                                                                                                 >  2:493916/1‑140 (MQ=255)                                      aacaacTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGGTAAACTGCTAACCTGTTCCTGGCAGCCAggg                                                                                               >  2:294754/1‑139 (MQ=255)                                           cTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgcgtgcg                                                                                          >  2:939877/1‑140 (MQ=255)                                               gTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCAc                                                                                      <  1:274132/140‑1 (MQ=255)                                                    aGATCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCAt                                                                                 <  1:4421/140‑1 (MQ=255)                                                    aGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCAt                                                                                 <  1:555415/140‑1 (MQ=255)                                                     gACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCAtt                                                                                >  2:711697/1‑140 (MQ=255)                                                      aCCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTa                                                                               >  2:891026/1‑140 (MQ=255)                                                           tACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCt                                                                          <  1:626031/140‑1 (MQ=255)                                                              tgctgTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAg                                                                       <  1:493916/140‑1 (MQ=255)                                                                  gtgGCATGGTCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGATGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAg                                                                       <  1:409391/136‑1 (MQ=255)                                                                  gtgGCATGGTCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGATGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAg                                                                       >  2:409391/1‑136 (MQ=255)                                                                     gCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAg                                                                       >  2:324026/1‑133 (MQ=255)                                                                     gCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAg                                                                       <  1:324026/133‑1 (MQ=255)                                                                          gCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCCGGCGTGCGGCACCGCATTACGCCCGAGGCGGTAGAtgct                                                           >  2:846261/1‑140 (MQ=255)                                                                          gCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGAtgct                                                           <  1:1138953/140‑1 (MQ=255)                                                                                acgaTGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGAtgc                                                            >  2:59741/1‑133 (MQ=255)                                                                                acgaTGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGAtgc                                                            <  1:59741/133‑1 (MQ=255)                                                                                  gaTGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGAc                                                   <  1:277959/140‑1 (MQ=255)                                                                                    tGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACAt                                                 >  2:344007/1‑140 (MQ=255)                                                                                    tGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACAt                                                 >  2:818434/1‑140 (MQ=255)                                                                                               ctgccGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAg                                      <  1:403411/140‑1 (MQ=255)                                                                                                tgccGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGc                                     <  1:846261/140‑1 (MQ=255)                                                                                                  ccGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCAt                                   >  2:652611/1‑140 (MQ=255)                                                                                                      cgaATCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCttt                               >  2:177637/1‑140 (MQ=255)                                                                                                         aTCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTaaa                            <  1:939877/140‑1 (MQ=255)                                                                                                          tCACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAac                           <  1:177637/140‑1 (MQ=255)                                                                                                           cACTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAaca                          <  1:711697/140‑1 (MQ=255)                                                                                                            aCTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAacac                         <  1:294754/140‑1 (MQ=255)                                                                                                             cTACGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACg                        >  2:355102/1‑140 (MQ=255)                                                                                                                cGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAg                     <  1:891026/140‑1 (MQ=255)                                                                                                                cGTTTGCAGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAg                     <  1:355102/140‑1 (MQ=255)                                                                                                                          agGCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATGCTGAcc           >  2:747917/1‑140 (MQ=255)                                                                                                                          agGCCTTAAGCGAGGCGGGACGGGATAAACTACTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATTCTGAcc           >  1:971604/1‑140 (MQ=255)                                                                                                                            gCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATGCTGAcccc         >  1:1036752/1‑140 (MQ=255)                                                                                                                            gCCTTAAGCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATGCTGAcccc         >  2:25822/1‑140 (MQ=255)                                                                                                                                   gCGAGGCGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATGCTGACCCCTTGCGCt  <  1:459547/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCACCGCATTACGCCTGAGGCGTTAGATGCTGCCGTGACATTTTTCCGCCAGCATCTTTAAACACGCAGAATGCTGACCCCTTGCGCT  >  NC_000913/4417459‑4417728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑