Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,836	G→C	A49P (GCC→CCC)	chpB →	endoribonuclease toxin ChpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,836	0	G	C	100.0%	287.7 / NA	79	A49P (GCC→CCC)	chpB	endoribonuclease toxin ChpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (41/38);  total (41/38)

AGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAA  >  NC_000913/4448699‑4448972                                                                                                                                          |                                                                                                                                         aGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGccc                                                                                                                                        >  1:1158229/1‑140 (MQ=255)  gAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGcccc                                                                                                                                       <  1:554315/140‑1 (MQ=255)   aaaaGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCcccc                                                                                                                                      >  2:389696/1‑140 (MQ=255)     aaGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAACAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCa                                                                                                                                    <  2:1158229/140‑1 (MQ=255)     aaGCGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCa                                                                                                                                    >  1:863690/1‑140 (MQ=255)        tGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTa                                                                                                                                 >  1:914238/1‑140 (MQ=255)            tttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCa                                                                                                                             <  2:427906/140‑1 (MQ=255)            tttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCa                                                                                                                             >  2:391209/1‑140 (MQ=255)            tttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCa                                                                                                                             <  1:509035/140‑1 (MQ=255)            tttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCa                                                                                                                             >  2:956527/1‑140 (MQ=255)             ttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCa                                                                                                                                    <  2:95703/132‑1 (MQ=255)             ttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCa                                                                                                                                    >  1:95703/1‑132 (MQ=255)             ttGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAg                                                                                                                            >  1:928593/1‑140 (MQ=255)                             tgtgCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGccc                                                                                                            >  1:110220/1‑140 (MQ=255)                             tgtgCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGccc                                                                                                            >  2:513647/1‑140 (MQ=255)                               tgCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGt                                                                                                          >  1:254761/1‑140 (MQ=255)                                 cTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTa                                                                                                        >  2:736/1‑140 (MQ=255)                                    gTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGc                                                                                                     >  1:19027/1‑140 (MQ=255)                                    gTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGc                                                                                                     >  2:292627/1‑140 (MQ=255)                                             gATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAg                                                                                            <  1:513647/140‑1 (MQ=255)                                               tCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                                                                          <  2:110220/140‑1 (MQ=255)                                               tCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                                                                          <  1:389696/140‑1 (MQ=255)                                               tCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCg                                                                                          <  2:296809/140‑1 (MQ=255)                                                  aGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTc                                                                                       >  1:846828/1‑140 (MQ=255)                                                    cAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCt                                                                                     <  2:269233/140‑1 (MQ=255)                                                        cGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTAc                                                                                 >  1:14161/1‑140 (MQ=255)                                                             aTGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGc                                                                            <  1:391209/140‑1 (MQ=255)                                                                     cAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGGATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGTTTTAGCGTTCCTTTACATTGCGAAGAAgg                                                                    >  2:178983/1‑140 (MQ=255)                                                                     cAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAgg                                                                    >  2:826785/1‑140 (MQ=255)                                                                       aGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCg                                                                  <  1:66752/140‑1 (MQ=255)                                                                       aGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCg                                                                  <  2:494854/140‑1 (MQ=255)                                                                        ggTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGa                                                                 >  1:349769/1‑140 (MQ=255)                                                                         gTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGAt                                                                >  2:218093/1‑140 (MQ=255)                                                                         gTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGAt                                                                <  2:863690/140‑1 (MQ=255)                                                                             tGGTCGACCTGCGCTTGTACTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCga                                                                          >  2:437003/1‑126 (MQ=255)                                                                             tGGTCGACCTGCGCTTGTACTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCga                                                                          <  1:437003/126‑1 (MQ=255)                                                                               gTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCgaagaa                                                                      <  2:43727/128‑1 (MQ=255)                                                                               gTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCgaagaa                                                                      >  1:43727/1‑128 (MQ=255)                                                                               gTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCAc                                                          >  2:489475/1‑140 (MQ=255)                                                                               gTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCAc                                                          >  2:348469/1‑140 (MQ=255)                                                                                tCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACg                                                         <  2:349769/140‑1 (MQ=255)                                                                                   aCCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCg                                                      <  1:684876/140‑1 (MQ=255)                                                                                   aCCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCg                                                      <  2:254761/140‑1 (MQ=255)                                                                                    ccTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCgt                                                     >  2:453618/1‑140 (MQ=255)                                                                                    ccTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTCTACATTGCGAAGAAGGCGATGTGCACGGCgt                                                     >  1:1047470/1‑140 (MQ=255)                                                                                      tGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCgtgg                                                   <  2:846828/140‑1 (MQ=255)                                                                                       gcgcTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCgtggt                                                  <  2:748584/140‑1 (MQ=255)                                                                                       gcgcTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCgtggt                                                  <  1:489475/140‑1 (MQ=255)                                                                                         gcTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGc                                                >  2:231266/1‑140 (MQ=255)                                                                                         gcTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGc                                                >  1:672710/1‑140 (MQ=255)                                                                                          cTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTCTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCt                                               <  2:1047470/140‑1 (MQ=255)                                                                                             gtgCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGt                                            >  1:264877/1‑140 (MQ=255)                                                                                             gtgCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGt                                            <  1:453618/140‑1 (MQ=255)                                                                                              tgCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTg                                           >  1:586790/1‑140 (MQ=255)                                                                                                 tctcCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAAt                                        <  2:672710/140‑1 (MQ=255)                                                                                                  ctcCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATc                                       >  2:851268/1‑140 (MQ=255)                                                                                                  ctcCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATc                                       >  1:301304/1‑140 (MQ=255)                                                                                                    ccGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAg                                     <  2:928593/140‑1 (MQ=255)                                                                                                      gTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGt                                   <  2:14161/140‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCgtggtg                                                 >  2:981136/1‑125 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCgtggtg                                                 <  1:981136/125‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGc                                                <  2:476838/126‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGc                                                >  1:476838/1‑126 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTg                                  >  1:285650/1‑140 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTg                                  <  2:301304/140‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTg                                  <  1:956527/140‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTg                                  <  1:231266/140‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTg                                  <  2:661252/140‑1 (MQ=255)                                                                                                       ttCAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTg                                  <  1:348469/140‑1 (MQ=255)                                                                                                         cAAGCCTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCg                                <  1:846275/140‑1 (MQ=255)                                                                                                             ccTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAAGGCGGAAATTTTGCCCGTGATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGgatg                            >  2:1031550/1‑140 (MQ=255)                                                                                                              cTTTAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGgatga                           <  1:949098/140‑1 (MQ=255)                                                                                                               tttAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGgatgat                          >  2:404901/1‑140 (MQ=255)                                                                                                               tttAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGgatgat                          <  1:851268/140‑1 (MQ=255)                                                                                                                ttAATCAACTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGatgatg                         <  1:1112495/140‑1 (MQ=255)                                                                                                                        cTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCt                    <  1:142313/137‑1 (MQ=255)                                                                                                                        cTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCt                    >  2:142313/1‑137 (MQ=255)                                                                                                                        cTGGGAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTaca                 >  2:162749/1‑140 (MQ=255)                                                                                                                           ggAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGgatga                           >  2:606988/1‑127 (MQ=255)                                                                                                                           ggAATGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGgatga                           <  1:606988/127‑1 (MQ=255)                                                                                                                             aaTGACGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGccc            >  2:171047/1‑140 (MQ=255)                                                                                                                                  cGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTg       >  1:996450/1‑140 (MQ=255)                                                                                                                                  cGCTGGTGCCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTg       >  1:598430/1‑140 (MQ=255)                                                                                                                                       gtgcCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCaaa  <  2:914238/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAA  >  NC_000913/4448699‑4448972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑