BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,512,215	A→G	V67A (GTG→GCG)	fecD ←	ferric citrate ABC transporter membrane subunit FecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,512,215	0	A	G	100.0%	422.4 / NA	114	V67A (GTG→GCG)	fecD	ferric citrate ABC transporter membrane subunit FecD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (61/53); total (61/53)

Only 100 of 116 total aligned reads displayed.

CGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCAT  >  NC_000913/4512079‑4512348

cGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCgg                                                                                                                                    >  1:610541/1‑140 (MQ=255)

         cATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTg                                                                                                                           <  2:443643/140‑1 (MQ=255)

           tCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCa                                                                                                                         <  1:1083733/140‑1 (MQ=255)

              cGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCg                                                                                                                      >  1:17415/1‑140 (MQ=255)

                ggCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGAc                                                                                                                    >  2:956842/1‑140 (MQ=255)

                ggCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGAc                                                                                                                    >  2:731871/1‑140 (MQ=255)

                     tGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACa                                                                                                               >  1:138133/1‑140 (MQ=255)

                       aCGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTg                                                                                                                           >  2:513319/1‑126 (MQ=255)

                           cATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCca                                                                                                         >  2:137366/1‑140 (MQ=255)

                           cATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCca                                                                                                         >  2:452368/1‑140 (MQ=255)

                              aagaagTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCcagca                                                                                                      <  2:254962/140‑1 (MQ=255)

                                gaagTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCagcagc                                                                                                    <  2:830559/140‑1 (MQ=255)

                                   gTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAg                                                                                                 >  1:511689/1‑140 (MQ=255)

                                     agagCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCTGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAgcg                                                                                               >  2:409800/1‑140 (MQ=255)

                                       agCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGcgcg                                                                                             <  2:543336/140‑1 (MQ=255)

                                        gCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCgg                                                                                            <  2:349285/140‑1 (MQ=255)

                                         cccccACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGc                                                                                           <  1:769852/140‑1 (MQ=255)

                                         cccccACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGc                                                                                           >  2:426863/1‑140 (MQ=255)

                                         cccccACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGc                                                                                           <  2:610541/140‑1 (MQ=255)

                                            ccACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGt                                                                                        <  2:156663/140‑1 (MQ=255)

                                              acaGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCg                                                                                      >  2:122050/1‑140 (MQ=255)

                                                 gagGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTa                                                                                   <  1:258938/140‑1 (MQ=255)

                                                 gagGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTa                                                                                   >  2:495980/1‑140 (MQ=255)

                                                 gagGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTa                                                                                   >  1:23998/1‑140 (MQ=255)

                                                  agGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTAc                                                                                  <  2:62430/140‑1 (MQ=255)

                                                   ggCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTAc                                                                                  >  2:698380/1‑139 (MQ=255)

                                                    gCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTa                                                                                   >  1:263624/1‑137 (MQ=255)

                                                    gCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTa                                                                                   <  2:263624/137‑1 (MQ=255)

                                                    gCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTAc                                                                                  <  2:847282/138‑1 (MQ=255)

                                                    gCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTAc                                                                                  >  1:847282/1‑138 (MQ=255)

                                                    gCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTc                                                                                >  2:648702/1‑140 (MQ=255)

                                                    gCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTc                                                                                >  1:910593/1‑140 (MQ=255)

                                                       aGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             <  2:511689/140‑1 (MQ=255)

                                                       aGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             <  2:427275/140‑1 (MQ=255)

                                                       aGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             <  1:1115355/140‑1 (MQ=255)

                                                       aGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             <  2:885651/140‑1 (MQ=255)

                                                       aGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             <  1:1066918/140‑1 (MQ=255)

                                                         gCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCa                                                                           <  2:138133/140‑1 (MQ=255)

                                                            ggcggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATa                                                                        >  1:321366/1‑140 (MQ=255)

                                                            ggcggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATa                                                                        <  2:17415/140‑1 (MQ=255)

                                                            ggcggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATa                                                                        <  2:895133/140‑1 (MQ=255)

                                                              cggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             <  2:860056/133‑1 (MQ=255)

                                                              cggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCAt                                                                             >  1:860056/1‑133 (MQ=255)

                                                              cggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACa                                                                      <  1:417749/140‑1 (MQ=255)

                                                                              gagaATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTc                                                      >  2:392800/1‑140 (MQ=255)

                                                                               agaATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCt                                                            <  1:840658/133‑1 (MQ=255)

                                                                               agaATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTcc                                                     >  1:150315/1‑140 (MQ=255)

                                                                               agaATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTcc                                                     >  2:1105101/1‑140 (MQ=255)

                                                                                    atCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCt                                                >  1:1058934/1‑140 (MQ=255)

                                                                                     tCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTg                                               >  2:247436/1‑140 (MQ=255)

                                                                                      ccGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGc                                              <  2:23998/140‑1 (MQ=255)

                                                                                         gTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑ACCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAg                                           <  1:547966/140‑1 (MQ=255)

                                                                                             tGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAg                                           <  1:193876/136‑1 (MQ=255)

                                                                                             tGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAg                                           >  2:193876/1‑136 (MQ=255)

                                                                                                       ttGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGcc                                             >  2:143375/1‑124 (MQ=255)

                                                                                                       ttGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGcc                                             <  1:143375/124‑1 (MQ=255)

                                                                                                        tGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAgcgcg                            >  1:533877/1‑140 (MQ=255)

                                                                                                        tGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAgcgcg                            >  1:985336/1‑140 (MQ=255)

                                                                                                        tGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAgcgcg                            >  2:597507/1‑140 (MQ=255)

                                                                                                        tGCGCACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAgcgcg                            <  1:247436/140‑1 (MQ=255)

                                                                                                          cgcACAATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAgcgcgcg                          >  1:424766/1‑140 (MQ=255)

                                                                                                             acaATCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCca                       <  1:426863/140‑1 (MQ=255)

                                                                                                             acaAGCCCCTGTATCAGCCCGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCca                       <  1:155335/140‑1 (MQ=255)

                                                                                                               aaTCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCcacg                     >  1:1096352/1‑140 (MQ=255)

                                                                                                                 tCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCcacggc                   >  2:497020/1‑140 (MQ=255)

                                                                                                                 tCCCCTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCcacggc                   >  2:34617/1‑140 (MQ=255)

                                                                                                                   cccTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCcacggcac                 <  2:119028/140‑1 (MQ=255)

                                                                                                                    ccTGTATCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCcacggcacg                >  1:827287/1‑140 (MQ=255)

                                                                                                                         aTCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           <  2:827287/140‑1 (MQ=255)

                                                                                                                         aTCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           >  2:681873/1‑140 (MQ=255)

                                                                                                                         aTCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           <  2:1096352/140‑1 (MQ=255)

                                                                                                                         aTCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           <  1:452368/140‑1 (MQ=255)

                                                                                                                         aTCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           <  1:495980/140‑1 (MQ=255)

                                                                                                                         aTCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCCGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           >  2:632599/1‑140 (MQ=255)

                                                                                                                          tCAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           >  1:472256/1‑139 (MQ=255)

                                                                                                                          ccccgccgcccggcCGCGCCGAGGCCTCCACCGACAAACCGTGCCAGCAGCACGCGCGGCCGTCGGTACTCCATCAATCCCAAATAATCCTCGTCACGGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACCGGGAt           <  2:575629/134‑1 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCca                       <  1:394301/126‑1 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCa         <  1:122050/140‑1 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCa         <  1:328591/140‑1 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCa         <  2:321366/140‑1 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCa         >  1:152266/1‑140 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCa         <  2:152266/140‑1 (MQ=255)

                                                                                                                           cAGCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCa         <  1:956842/140‑1 (MQ=255)

                                                                                                                             gCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACt       >  2:503719/1‑140 (MQ=255)

                                                                                                                             gCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACt       >  1:260772/1‑140 (MQ=255)

                                                                                                                             gCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACt       <  1:681873/140‑1 (MQ=255)

                                                                                                                             gCACGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACt       <  1:1096540/140‑1 (MQ=255)

                                                                                                                               aCGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTcc     >  2:545376/1‑140 (MQ=255)

                                                                                                                               aCGCCC‑GCCGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACT‑CATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTccc    >  2:809560/1‑140 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           <  1:249853/131‑1 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGAt           >  2:249853/1‑131 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  >  1:1008117/1‑140 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  >  1:237116/1‑140 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  <  2:406776/140‑1 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  >  1:575629/1‑140 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  >  1:641027/1‑140 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  >  1:867126/1‑140 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCat  <  2:1147479/140‑1 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCa   <  1:913084/139‑1 (MQ=255)

                                                                                                                                  ccc‑gccGCGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCa   >  2:913084/1‑139 (MQ=255)

CGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCC‑GCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCAT  >  NC_000913/4512079‑4512348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`