Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,616,640	T→C	I142M (ATA→ATG)	yjjI ←	DUF3029 domain‑containing protein YjjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,616,640	0	T	C	100.0%	338.2 / NA	91	I142M (ATA→ATG)	yjjI	DUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/50);  total (41/50)

TCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCG  >  NC_000913/4616504‑4616775                                                                                                                                         |                                                                                                                                        tCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCAtt                                                                                                                                      >  1:998244/1‑140 (MQ=255)   aGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCg                                                                                                                                    <  2:922041/140‑1 (MQ=255)        cGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACAt                                                                                                                               <  1:98206/140‑1 (MQ=255)          gTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGTGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCg                                                                                                                             <  1:1096036/140‑1 (MQ=255)          gTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCg                                                                                                                             >  1:438062/1‑140 (MQ=255)          gTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCg                                                                                                                             >  1:27061/1‑140 (MQ=255)            gAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGAt                                                                                                                           <  2:983616/140‑1 (MQ=255)                  tGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTc                                                                                                                     <  1:113271/140‑1 (MQ=255)                  tGCTTCAAATCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTc                                                                                                                     <  2:1087930/140‑1 (MQ=255)                    cTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGTAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCtt                                                                                                                   >  2:803947/1‑140 (MQ=255)                    cTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCtt                                                                                                                   >  2:350315/1‑140 (MQ=255)                         aCTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGtt                                                                                                              >  1:1117451/1‑140 (MQ=255)                         aCTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGtt                                                                                                              >  1:689228/1‑140 (MQ=255)                             tgcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCCGAAACGTTTCATTCGAACATCGATCTCGTCTTGCGTTAGaa                                                                                                          >  2:634740/1‑140 (MQ=255)                             tgcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGaa                                                                                                          <  1:837618/140‑1 (MQ=255)                             tgcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGaa                                                                                                          <  2:381377/140‑1 (MQ=255)                             tgcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGaa                                                                                                          >  2:495297/1‑140 (MQ=255)                              gcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGa                                                                                                           >  1:328205/1‑138 (MQ=255)                              gcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGa                                                                                                           <  2:328205/138‑1 (MQ=255)                              gcatctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAAt                                                                                                         >  2:226409/1‑140 (MQ=255)                                     caCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTtg                                                                                                                  <  1:741573/124‑1 (MQ=255)                                     caCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTtg                                                                                                                  >  2:741573/1‑124 (MQ=255)                                     caCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa                                                                                                  >  1:117446/1‑140 (MQ=255)                                     caCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa                                                                                                  <  1:405066/140‑1 (MQ=255)                                      aCGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACAt                                                                                                 <  2:118768/140‑1 (MQ=255)                                         tAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACg                                                                                              >  2:190041/1‑140 (MQ=255)                                         tAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACg                                                                                              >  1:1053326/1‑140 (MQ=255)                                         tAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACg                                                                                              >  2:632698/1‑140 (MQ=255)                                             aTCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTg                                                                                          <  2:27061/140‑1 (MQ=255)                                                 cgcgGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGcaac                                                                                      <  2:501888/140‑1 (MQ=255)                                                 cgcgGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGcaac                                                                                      >  2:744229/1‑140 (MQ=255)                                                  gcgGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACAt                                                                                                 <  1:243079/128‑1 (MQ=255)                                                  gcgGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACAt                                                                                                 >  2:243079/1‑128 (MQ=255)                                                  gcgGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGcaaca                                                                                     >  2:409328/1‑140 (MQ=255)                                                   cgGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGcaacaa                                                                                    <  1:1092098/140‑1 (MQ=255)                                                          tGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATcc                                                                             >  1:636337/1‑140 (MQ=255)                                                            ggggAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAg                                                                           <  1:73629/137‑1 (MQ=255)                                                            ggCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAGCATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAg                                                                           <  1:634740/140‑1 (MQ=255)                                                            ggCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAg                                                                           >  1:346459/1‑140 (MQ=255)                                                              cGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGtt                                                                         >  1:585112/1‑140 (MQ=255)                                                              cGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGtt                                                                         >  2:522737/1‑140 (MQ=255)                                                                aaTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGc                                                                       >  1:203077/1‑140 (MQ=255)                                                                aaTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGc                                                                       >  2:73573/1‑140 (MQ=255)                                                                 aTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAGCAACGCATCCAGTTGcc                                                                      <  2:117446/140‑1 (MQ=255)                                                                 aTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGcc                                                                      <  1:1016333/140‑1 (MQ=255)                                                                 aTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGcc                                                                      >  2:770337/1‑140 (MQ=255)                                                                 aTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGcc                                                                      <  1:497494/140‑1 (MQ=255)                                                                      aCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAg                                                                           <  1:3324/130‑1 (MQ=255)                                                                      aCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAg                                                                           >  2:3324/1‑130 (MQ=255)                                                                       cGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGt                                                                <  1:190041/140‑1 (MQ=255)                                                                          gCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGa                                                             >  1:589712/1‑140 (MQ=255)                                                                           ccGATATTGGCGTGCATAAAGGCGTCTGGTAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc                                                            <  1:803947/140‑1 (MQ=255)                                                                           ccGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc                                                            <  2:203077/140‑1 (MQ=255)                                                                               tatTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGc                                                        <  1:522737/140‑1 (MQ=255)                                                                                atTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCa                                                       <  1:770337/140‑1 (MQ=255)                                                                                    gCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGa                                                   >  1:544926/1‑140 (MQ=255)                                                                                    gCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGa                                                   >  1:734859/1‑140 (MQ=255)                                                                                    gCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGa                                                   >  2:552028/1‑140 (MQ=255)                                                                                    gCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGa                                                   >  1:392517/1‑140 (MQ=255)                                                                                        gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgtg                                               <  1:226409/140‑1 (MQ=255)                                                                                         cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATTCGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa                                              >  1:429672/1‑140 (MQ=255)                                                                                         cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa                                              >  2:315182/1‑140 (MQ=255)                                                                                         cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa                                              >  2:522476/1‑140 (MQ=255)                                                                                         cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa                                              >  1:1150561/1‑140 (MQ=255)                                                                                         cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa                                              <  1:522476/140‑1 (MQ=255)                                                                                           tAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                                            <  2:585112/140‑1 (MQ=255)                                                                                            aaaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCg                                           <  1:234056/140‑1 (MQ=255)                                                                                            aaaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCg                                           <  1:615620/140‑1 (MQ=255)                                                                                             aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGa                                          <  1:350315/140‑1 (MQ=255)                                                                                             aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGa                                          <  1:315182/140‑1 (MQ=255)                                                                                             aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGa                                          <  2:346459/140‑1 (MQ=255)                                                                                                   tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAc                                    >  2:66573/1‑140 (MQ=255)                                                                                                    cTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                                   <  2:734859/140‑1 (MQ=255)                                                                                                    cTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                                   <  2:1150561/140‑1 (MQ=255)                                                                                                      ggCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                                 <  2:636337/140‑1 (MQ=255)                                                                                                      ggCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                                 <  1:66573/140‑1 (MQ=255)                                                                                                      ggCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                                 <  2:589712/140‑1 (MQ=255)                                                                                                                tGTCGAGGTAACGCCAGAAACCTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGa                       <  2:1053326/140‑1 (MQ=255)                                                                                                                 gTCGAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGaa                      >  1:509242/1‑140 (MQ=255)                                                                                                                    gAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg                   <  1:495297/140‑1 (MQ=255)                                                                                                                    gAGGTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg                   <  1:632698/140‑1 (MQ=255)                                                                                                                      ggTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTc                 <  2:544926/140‑1 (MQ=255)                                                                                                                       gTAATGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGt                  <  2:226123/138‑1 (MQ=255)                                                                                                                       gTAATGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGt                  >  1:226123/1‑138 (MQ=255)                                                                                                                       gTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg                   >  1:254663/1‑137 (MQ=255)                                                                                                                       gTAACGCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg                   <  2:254663/137‑1 (MQ=255)                                                                                                                            gCCAGAAACGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCaga           >  1:176758/1‑140 (MQ=255)                                                                                                                                    cGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGc   <  2:998244/140‑1 (MQ=255)                                                                                                                                    cGTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGc   <  1:308890/140‑1 (MQ=255)                                                                                                                                     gTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCg  <  2:358147/140‑1 (MQ=255)                                                                                                                                     gTTTCATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCg  <  2:1117451/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCG  >  NC_000913/4616504‑4616775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑