Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	465,107	A→G	S147S (AGT→AGC)	queC ←	7‑cyano‑7‑deazaguanine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	465,107	0	A	G	100.0%	241.6 / NA	65	S147S (AGT→AGC)	queC	7‑cyano‑7‑deazaguanine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/30);  total (35/30)

CGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG  >  NC_000913/464977‑465239                                                                                                                                   |                                                                                                                                     cGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCa                                                                                                                             <  2:457764/140‑1 (MQ=255) cGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCa                                                                                                                             <  1:165887/140‑1 (MQ=255)       aGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGttt                                                                                                                       <  2:428839/140‑1 (MQ=255)            gTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCt                                                                                                                                    >  1:1062852/1‑122 (MQ=255)             tCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGc                                                                                                                              >  2:423351/1‑127 (MQ=255)             tCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGc                                                                                                                              <  1:423351/127‑1 (MQ=255)             tCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCa                                                                                                                             <  1:309273/128‑1 (MQ=255)             tCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCa                                                                                                                             >  2:309273/1‑128 (MQ=255)             tCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCt                                                                                                                 >  2:315562/1‑140 (MQ=255)                             gACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATcgc                                                                                                 >  1:203146/1‑140 (MQ=255)                              aCTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATcgcg                                                                                                <  2:443929/140‑1 (MQ=255)                              aCTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATcgcg                                                                                                <  2:273757/140‑1 (MQ=255)                              aCTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATcgcg                                                                                                >  1:443929/1‑140 (MQ=255)                               cTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCgg                                                                                               <  1:618485/140‑1 (MQ=255)                                 aaaTCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCa                                                                                             >  1:758128/1‑140 (MQ=255)                                       aGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCgg                                                                                       <  1:589015/140‑1 (MQ=255)                                        gTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCggg                                                                                      >  2:816095/1‑140 (MQ=255)                                        gTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCggg                                                                                      >  2:148559/1‑140 (MQ=255)                                        gTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCggg                                                                                      >  2:623830/1‑140 (MQ=255)                                                   taatCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGaa                                                                           >  2:723784/1‑140 (MQ=255)                                                          ccAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGtt                                                                    >  2:411063/1‑140 (MQ=255)                                                            aGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTc                                                                  >  1:697650/1‑140 (MQ=255)                                                             gcgcCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGTAATCCGGGTAGCCGGAGAAATCCGTTTCg                                                                 <  1:1122834/140‑1 (MQ=255)                                                              cgcCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGc                                                                >  2:1128271/1‑140 (MQ=255)                                                               gcCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCa                                                               <  1:723784/140‑1 (MQ=255)                                                               gcCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCa                                                               <  1:623830/140‑1 (MQ=255)                                                                    ggTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGc                                                          >  1:125032/1‑140 (MQ=255)                                                                            cTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAAtt                                                  <  2:697650/140‑1 (MQ=255)                                                                             tttATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa                                                 <  2:758128/140‑1 (MQ=255)                                                                              ttATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAc                                                <  1:272653/140‑1 (MQ=255)                                                                                   aaTCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCtt                                           <  1:816095/140‑1 (MQ=255)                                                                                        acaTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCt                                      <  2:125032/140‑1 (MQ=255)                                                                                         caTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa                                                 <  2:412911/128‑1 (MQ=255)                                                                                         caTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa                                                 >  1:412911/1‑128 (MQ=255)                                                                                         caTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCtt                                     >  1:956343/1‑140 (MQ=255)                                                                                         caTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCtt                                     >  2:560394/1‑140 (MQ=255)                                                                                          aTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCttt                                    >  1:116496/1‑140 (MQ=255)                                                                                          aTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCttt                                    >  1:989393/1‑140 (MQ=255)                                                                                          aTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCttt                                    >  2:486990/1‑140 (MQ=255)                                                                                           tCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCtttt                                   >  1:1150124/1‑140 (MQ=255)                                                                                               cggcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCt                               >  2:969960/1‑140 (MQ=255)                                                                                               cggcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCt                               >  1:557399/1‑140 (MQ=255)                                                                                               cggcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCt                               >  1:23110/1‑140 (MQ=255)                                                                                                ggcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTg                              <  2:1150124/140‑1 (MQ=255)                                                                                                ggcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTg                              <  2:696455/140‑1 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTc                                          <  1:513365/127‑1 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTc                                          >  2:513365/1‑127 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             >  2:549357/1‑140 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             <  2:989393/140‑1 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             <  2:114452/140‑1 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             <  1:969960/140‑1 (MQ=255)                                                                                                 gcgTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             <  1:560394/140‑1 (MQ=255)                                                                                                   gTTTCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATa                           <  1:486990/140‑1 (MQ=255)                                                                                                    tttCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             >  2:435343/1‑137 (MQ=255)                                                                                                    tttCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             <  2:544732/137‑1 (MQ=255)                                                                                                    tttCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             >  1:544732/1‑137 (MQ=255)                                                                                                    tttCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGa                             <  1:435343/137‑1 (MQ=255)                                                                                                    tttCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATAc                          >  1:134813/1‑140 (MQ=255)                                                                                                     ttCAAAACGAATATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACg                         <  2:557399/140‑1 (MQ=255)                                                                                                              aaTATCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTg                >  2:729941/1‑140 (MQ=255)                                                                                                               atatCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTgc               >  2:308086/1‑140 (MQ=255)                                                                                                               atatCTTTCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTgc               >  2:751623/1‑140 (MQ=255)                                                                                                                      tCGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGc        <  2:23110/140‑1 (MQ=255)                                                                                                                       cGCCATGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCg       >  1:757894/1‑140 (MQ=255)                                                                                                                            tGCCCAAGCTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAgg  >  2:755212/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     CGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG  >  NC_000913/464977‑465239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑