Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,378	T→C	T676A (ACT→GCT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,378	0	T	C	100.0%	184.7 / NA	51	T676A (ACT→GCT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/20);  total (31/20)

AGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTC  >  NC_000913/482242‑482511                                                                                                                                         |                                                                                                                                      aGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCAcc                                                                                                                                    <  1:900493/140‑1 (MQ=255) aGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCAcc                                                                                                                                    <  2:408963/140‑1 (MQ=255)  gACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCAccc                                                                                                                                   <  2:65521/140‑1 (MQ=255)   aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCa                                                                                                                                  >  2:493738/1‑140 (MQ=255)   aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCa                                                                                                                                  >  1:670912/1‑140 (MQ=255)     cGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGt                                                                                                                                >  1:995987/1‑140 (MQ=255)       tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTc                                                                                                                              >  2:691132/1‑140 (MQ=255)       tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTc                                                                                                                              >  1:630097/1‑140 (MQ=255)               tACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTg                                                                                                                      >  1:1027269/1‑140 (MQ=255)                 cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCg                                                                                                                    <  2:331076/140‑1 (MQ=255)                   cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCggg                                                                                                                  <  2:995987/140‑1 (MQ=255)                   cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCggg                                                                                                                  <  1:691132/140‑1 (MQ=255)                     ggTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCa                                                                                                                >  1:1122255/1‑140 (MQ=255)                                cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCggg                                                                                                                  >  1:202920/1‑127 (MQ=255)                                cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCggg                                                                                                                  <  2:202920/127‑1 (MQ=255)                                     tGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAAc                                                                                                >  1:73189/1‑140 (MQ=255)                                     tGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAAc                                                                                                >  2:131774/1‑140 (MQ=255)                                     tGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAAc                                                                                                >  2:141434/1‑140 (MQ=255)                                        ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt                                                                                             >  1:467272/1‑140 (MQ=255)                                        ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt                                                                                             >  1:310289/1‑140 (MQ=255)                                                 tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCttt                                                                                    >  2:875/1‑140 (MQ=255)                                                    gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGAt                                                                                 <  2:467272/140‑1 (MQ=255)                                                    gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGAt                                                                                 >  2:1042677/1‑140 (MQ=255)                                                          aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCga                                                                           >  2:893201/1‑140 (MQ=255)                                                                  aCGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGGAAgcgc                                                                   <  1:980287/140‑1 (MQ=255)                                                                  aCGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgcgc                                                                   >  1:51135/1‑140 (MQ=255)                                                                   cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGcgcg                                                                  <  2:51135/140‑1 (MQ=255)                                                                    gcgcCTGAGTCAGTTTTTCGTTACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCgt                                                                 >  1:685405/1‑140 (MQ=255)                                                                       ccTGAGTCAGTTTTTCGTTACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGtt                                                              <  2:685405/140‑1 (MQ=255)                                                                          gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCACTCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                           <  2:798915/140‑1 (MQ=255)                                                                          gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCGCGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                           <  2:1122255/140‑1 (MQ=255)                                                                          gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                           <  2:310289/140‑1 (MQ=255)                                                                          gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                           >  1:141506/1‑140 (MQ=255)                                                                              cAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCa                                                       >  1:259194/1‑140 (MQ=255)                                                                                    ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTaa                                                 >  1:1106074/1‑140 (MQ=255)                                                                                          aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCtt                                           >  1:559401/1‑140 (MQ=255)                                                                                          aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCtt                                           <  1:1042677/140‑1 (MQ=255)                                                                                              aGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAAc                                       <  2:259194/140‑1 (MQ=255)                                                                                                     ccTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGttt                                >  2:708334/1‑140 (MQ=255)                                                                                                     ccTGGTCAATCAGCTC‑AAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGtttt                               >  1:598331/1‑140 (MQ=255)                                                                                                          tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttcttc                           >  1:158572/1‑140 (MQ=255)                                                                                                          tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttcttc                           >  1:404923/1‑140 (MQ=255)                                                                                                                   tcaaagtcaaagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACg                  >  1:122078/1‑140 (MQ=255)                                                                                                                   tcaaagtcaaagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACg                  >  2:366894/1‑140 (MQ=255)                                                                                                                   tcaaagtcaaagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACg                  <  1:493738/140‑1 (MQ=255)                                                                                                                   tcaaagtcaaagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACg                  >  2:967208/1‑140 (MQ=255)                                                                                                                    caaagtc‑aagcCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGAt                <  1:1059604/140‑1 (MQ=255)                                                                                                                     aaagtcaaagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGAt                <  2:73189/140‑1 (MQ=255)                                                                                                                          caaagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGcc           <  2:404923/140‑1 (MQ=255)                                                                                                                            aagCCGGTTGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCa         <  2:598331/140‑1 (MQ=255)                                                                                                                                   ttGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTc  >  2:20205/1‑140 (MQ=255)                                                                                                                                   ttGCAGCACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTc  >  1:1029381/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTC  >  NC_000913/482242‑482511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑