Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,735	G→C	V274L (GTG→CTG)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,735	0	G	C	100.0%	335.3 / NA	91	V274L (GTG→CTG)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (47/44);  total (47/44)

ACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTA  >  NC_000913/534599‑534867                                                                                                                                         |                                                                                                                                     aCTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGctgt                                                                                                                                   <  1:728444/140‑1 (MQ=255)     aCCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTgg                                                                                                                               >  1:225158/1‑140 (MQ=255)     aCCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTgg                                                                                                                               >  2:316304/1‑140 (MQ=255)       cAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTa                                                                                                                             <  1:349551/140‑1 (MQ=255)          cGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCg                                                                                                                          >  2:45603/1‑140 (MQ=255)            ttCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGt                                                                                                                        <  1:370573/140‑1 (MQ=255)              ccGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTaa                                                                                                                      <  1:45603/140‑1 (MQ=255)                    aTCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGttt                                                                                                                >  1:578337/1‑140 (MQ=255)                        cAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCt                                                                                                            <  2:416867/140‑1 (MQ=255)                         aaCGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTa                                                                                                           >  1:596413/1‑140 (MQ=255)                         aaCGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTa                                                                                                           <  2:306797/140‑1 (MQ=255)                           cGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAAc                                                                                                         >  2:197048/1‑140 (MQ=255)                               aaTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTaa                                                                                                                      >  1:797383/1‑123 (MQ=255)                               aaTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTaa                                                                                                                      <  2:797383/123‑1 (MQ=255)                                aTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGt                                                                                                                  >  2:761119/1‑126 (MQ=255)                                aTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGt                                                                                                                  <  1:761119/126‑1 (MQ=255)                                aTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCa                                                                                                    >  1:1031820/1‑140 (MQ=255)                                   ggctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAc                                                                                                 <  1:316304/140‑1 (MQ=255)                                   ggctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAc                                                                                                 >  2:879012/1‑140 (MQ=255)                                   ggctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAc                                                                                                 <  2:225158/140‑1 (MQ=255)                                   ggctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAc                                                                                                 <  1:672169/140‑1 (MQ=255)                                   ggctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATAc                                                                                                 >  2:248518/1‑140 (MQ=255)                                     ctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCg                                                                                               >  1:777506/1‑140 (MQ=255)                                     ctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCg                                                                                               >  1:579683/1‑140 (MQ=255)                                      tggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                              <  2:596413/140‑1 (MQ=255)                                      tggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                              <  2:53634/140‑1 (MQ=255)                                       ggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGc                                                                                             >  1:116592/1‑140 (MQ=255)                                        gggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            <  2:777506/140‑1 (MQ=255)                                        gggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            <  2:578337/140‑1 (MQ=255)                                        gggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGAAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            <  1:1013604/140‑1 (MQ=255)                                              tATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTag                                                                                      >  2:166149/1‑140 (MQ=255)                                              tATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTag                                                                                      >  2:46591/1‑140 (MQ=255)                                                tCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTagag                                                                                    <  2:255893/140‑1 (MQ=255)                                                  ccGGACGATCATGAACTTATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGaa                                                                                  <  2:1031820/140‑1 (MQ=255)                                                    ggACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            >  1:61234/1‑128 (MQ=255)                                                    ggACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            <  2:61234/128‑1 (MQ=255)                                                     gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGAAGAGAAATa                                                                               >  2:609236/1‑140 (MQ=255)                                                       cGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATaca                                                                             <  2:528757/140‑1 (MQ=255)                                                        gATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                              <  1:1087995/122‑1 (MQ=255)                                                        gATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                              >  2:1087995/1‑122 (MQ=255)                                                        gATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            >  1:189241/1‑124 (MQ=255)                                                        gATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCt                                                                                            <  2:189241/124‑1 (MQ=255)                                                          tCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCg                                                                          >  1:576202/1‑140 (MQ=255)                                                                   gATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCa                                                                 <  1:248518/140‑1 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:621407/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:442355/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:90103/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  1:122124/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:300740/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:266396/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  1:130646/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  1:212181/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:655511/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  2:866801/1‑140 (MQ=255)                                                                    aTGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaa                                                                >  1:810392/1‑140 (MQ=255)                                                                        ccGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATc                                                            >  1:840064/1‑140 (MQ=255)                                                                           ggATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGtt                                                         <  1:715041/140‑1 (MQ=255)                                                                           ggATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGtt                                                         <  1:655511/140‑1 (MQ=255)                                                                           ggATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGtt                                                         <  1:501718/140‑1 (MQ=255)                                                                            gATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTc                                                        >  2:401533/1‑140 (MQ=255)                                                                             aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCa                                                       <  1:46591/140‑1 (MQ=255)                                                                             aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCa                                                       <  1:300740/140‑1 (MQ=255)                                                                             aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCa                                                       <  1:477977/140‑1 (MQ=255)                                                                             aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCa                                                       <  2:461548/140‑1 (MQ=255)                                                                              tggtggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCat                                                      >  1:75417/1‑140 (MQ=255)                                                                               ggtggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCata                                                     <  2:116592/140‑1 (MQ=255)                                                                                       tGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgatatt                                             >  2:107032/1‑140 (MQ=255)                                                                                        gCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgatattg                                            >  1:700352/1‑140 (MQ=255)                                                                                         cAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCAtattgatattga                                           >  1:577777/1‑140 (MQ=255)                                                                                          aaaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAg                                          <  2:577777/140‑1 (MQ=255)                                                                                           aaCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGc                                         >  2:1156297/1‑140 (MQ=255)                                                                                            aCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGcc                                        <  1:1156297/140‑1 (MQ=255)                                                                                              cgcgCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGa                                      <  2:311770/140‑1 (MQ=255)                                                                                              cgcgCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGa                                      <  1:609236/140‑1 (MQ=255)                                                                                               gcgcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGAc                                     >  2:636372/1‑140 (MQ=255)                                                                                                       ttACGGTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTg                             <  1:266396/140‑1 (MQ=255)                                                                                                           ggTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTcg                         <  2:130646/140‑1 (MQ=255)                                                                                                            gTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTcgc                        <  2:212181/140‑1 (MQ=255)                                                                                                            gTAACGCAACGCTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTcgc                        <  2:700352/140‑1 (MQ=255)                                                                                                               acgcaacgcTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTg                     <  2:75417/140‑1 (MQ=255)                                                                                                               acgcaacgcTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTg                     >  2:811104/1‑140 (MQ=255)                                                                                                                 gcaacgcTGCTGGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCt                   >  1:234658/1‑140 (MQ=255)                                                                                                                          ctgGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTg                     <  2:77310/129‑1 (MQ=255)                                                                                                                          ctgGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTg                     >  1:77310/1‑129 (MQ=255)                                                                                                                          ctgGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGa          >  1:91443/1‑140 (MQ=255)                                                                                                                           tgGCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAt         >  2:734860/1‑140 (MQ=255)                                                                                                                             gCGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAtct       <  2:91443/140‑1 (MQ=255)                                                                                                                              cGTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAtctc      <  1:442355/140‑1 (MQ=255)                                                                                                                               gTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCg            <  2:53048/133‑1 (MQ=255)                                                                                                                               gTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCg            >  1:53048/1‑133 (MQ=255)                                                                                                                               gTCTGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCg     <  2:810392/140‑1 (MQ=255)                                                                                                                                  tGACATGCTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTa  >  2:229652/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     ACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTA  >  NC_000913/534599‑534867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑