Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	560,984	A→G	T430A (ACG→GCG)	sfmD →	putative fimbrial usher protein SfmD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	560,984	0	A	G	100.0%	248.2 / NA	69	T430A (ACG→GCG)	sfmD	putative fimbrial usher protein SfmD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/36);  total (33/36)

CAGGGTACGGCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAACGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTT  >  NC_000913/560849‑561117                                                                                                                                        |                                                                                                                                      cAGGGTACGGCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCa                                                                                                                                   <  2:998281/140‑1 (MQ=255) cAGGGTACGGCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCa                                                                                                                                   <  2:724495/140‑1 (MQ=255)        cGGCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCGCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATgcgcgc                                                                                                                            <  1:685696/140‑1 (MQ=255)         ggCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGca                                                                                                                           <  2:128017/140‑1 (MQ=255)              tCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTTTTAGGGGTGGGGGGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcagtcag                                                                                                                      >  1:179660/1‑140 (MQ=255)              tCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcagtcag                                                                                                                      <  1:1142484/140‑1 (MQ=255)              tCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcagtcag                                                                                                                      >  1:703310/1‑140 (MQ=255)              tCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGca                                                                                                                           >  1:84951/1‑135 (MQ=255)              tCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGca                                                                                                                           <  2:84951/135‑1 (MQ=255)               cggcggTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcagtcagt                                                                                                                     <  1:683456/140‑1 (MQ=255)               cggcggTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcagtcagt                                                                                                                     <  1:654698/140‑1 (MQ=255)                ggcggTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcag                                                                                                                          <  2:901286/134‑1 (MQ=255)                ggcggTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGcag                                                                                                                          >  1:901286/1‑134 (MQ=255)                ggcggTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGtt                                                                                                                    >  2:633765/1‑140 (MQ=255)                ggcggTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGtt                                                                                                                    >  2:87528/1‑140 (MQ=255)                         ccACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGc                                                                                                           <  1:534003/140‑1 (MQ=255)                             aGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAgt                                                                                                       <  1:633765/140‑1 (MQ=255)                             aGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAgt                                                                                                       <  2:703310/140‑1 (MQ=255)                              ggAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAgtc                                                                                                      <  2:845780/140‑1 (MQ=255)                                      cTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAg                                                                                              <  1:87528/140‑1 (MQ=255)                                        gCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAggg                                                                                            <  1:12785/140‑1 (MQ=255)                                          cTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAgtcgtc                                                                                                   <  1:854428/131‑1 (MQ=255)                                          cTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAgtcgtc                                                                                                   >  2:854428/1‑131 (MQ=255)                                          cTACGGCGGGACACAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAggggg                                                                                          <  2:405906/140‑1 (MQ=255)                                            aCGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGAt                                                                                        >  1:586095/1‑140 (MQ=255)                                                cggGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTa                                                                                    <  2:1087773/140‑1 (MQ=255)                                                   gACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTc                                                                                 >  1:938165/1‑140 (MQ=255)                                                   gACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTc                                                                                 >  2:4592/1‑140 (MQ=255)                                                      gCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCt                                                                              <  2:525781/140‑1 (MQ=255)                                                               tGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATg                                                                     >  2:705533/1‑140 (MQ=255)                                                               tGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATg                                                                     >  1:605433/1‑140 (MQ=255)                                                                  cAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGTGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGa                                                                  >  1:800280/1‑140 (MQ=255)                                                                  cAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGa                                                                  >  2:347333/1‑140 (MQ=255)                                                                    aTTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGaaa                                                                <  2:179660/140‑1 (MQ=255)                                                                       acacCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATc                                                              <  2:1003894/139‑1 (MQ=255)                                                                       acacCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATc                                                              >  1:1003894/1‑139 (MQ=255)                                                                        cacCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGa                                                            <  1:347333/140‑1 (MQ=255)                                                                           cGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAAt                                                               <  2:114302/134‑1 (MQ=255)                                                                           cGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAAt                                                               >  1:114302/1‑134 (MQ=255)                                                                           cGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGa                                                         >  2:431398/1‑140 (MQ=255)                                                                            gCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGaa                                                        <  1:705533/140‑1 (MQ=255)                                                                             ccTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAac                                                       >  2:1045810/1‑140 (MQ=255)                                                                             ccTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAac                                                       >  2:1015207/1‑140 (MQ=255)                                                                             ccTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAac                                                       <  2:592183/140‑1 (MQ=255)                                                                                       aGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCa                                             >  2:402337/1‑140 (MQ=255)                                                                                        gggCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCAc                                            >  2:137371/1‑140 (MQ=255)                                                                                        gggCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCAc                                            >  2:410100/1‑140 (MQ=255)                                                                                          gCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGTGAAATCGATGAac                                                       >  2:885450/1‑127 (MQ=255)                                                                                          gCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGTGAAATCGATGAac                                                       <  1:885450/127‑1 (MQ=255)                                                                                           cTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCaa                                         >  1:940069/1‑140 (MQ=255)                                                                                              gggCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAAttt                                      >  2:629988/1‑140 (MQ=255)                                                                                               ggCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAAtttt                                     >  2:388103/1‑140 (MQ=255)                                                                                                 cgcAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCa                                   <  1:4592/140‑1 (MQ=255)                                                                                                   cAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGt                                 >  1:1006351/1‑140 (MQ=255)                                                                                                    aaTCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAAtttt                                     >  1:962632/1‑135 (MQ=255)                                                                                                    aaTCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAAtttt                                     <  2:962632/135‑1 (MQ=255)                                                                                                      tctcGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGTGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGCTTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTaa                              <  2:800280/140‑1 (MQ=255)                                                                                                         cGGGAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATgg                           >  2:848576/1‑140 (MQ=255)                                                                                                           ggAACTGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGt                         <  2:814160/140‑1 (MQ=255)                                                                                                                tGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCg                    >  2:565327/1‑140 (MQ=255)                                                                                                                tGGGGCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCg                    <  2:1006351/140‑1 (MQ=255)                                                                                                                tGGGGCACAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCg                    >  2:807626/1‑140 (MQ=255)                                                                                                                  gggCGCAGTGTCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCt                  >  2:891828/1‑140 (MQ=255)                                                                                                                            tCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCg                    >  2:266817/1‑128 (MQ=255)                                                                                                                            tCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCg                    <  1:266817/128‑1 (MQ=255)                                                                                                                            tCGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGAcaca        >  2:279127/1‑140 (MQ=255)                                                                                                                             cGCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACaa       <  2:873135/140‑1 (MQ=255)                                                                                                                              gCTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAg      <  1:807626/140‑1 (MQ=255)                                                                                                                               cTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAgg     <  1:565327/140‑1 (MQ=255)                                                                                                                               cTGGATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAgg     <  1:629988/140‑1 (MQ=255)                                                                                                                                  gATGTAGCGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGttt  >  2:471561/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CAGGGTACGGCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGCGGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAATCTCGGGAACTGGGGCGCAGTGTCGCTGGATGTAACGCATGCGCGCAGTCAGTTAGCCGACGCCAGTCGTCATGAGGGGGATTCTATTCGCTTCCTCTATGCGAAATCGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTT  >  NC_000913/560849‑561117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑