Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	639,292	T→C	G116G (GGT→GGC)	ahpC →	alkyl hydroperoxide reductase, AhpC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	639,292	0	T	C	100.0%	209.0 / NA	57	G116G (GGT→GGC)	ahpC	alkyl hydroperoxide reductase, AhpC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/33);  total (24/33)

TATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGTCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTAGCTTCTCAC  >  NC_000913/639156‑639427                                                                                                                                         |                                                                                                                                        tATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTg                                                                                                                                      >  2:11234/1‑140 (MQ=255)   tCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGc                                                                                                                                    <  1:549011/140‑1 (MQ=255)     tACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTg                                                                                                                                  <  1:1142449/140‑1 (MQ=255)      aCCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGa                                                                                                                                 >  1:502536/1‑140 (MQ=255)         gATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCg                                                                                                                              >  2:332580/1‑140 (MQ=255)           tACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTg                                                                                                                            >  1:266039/1‑140 (MQ=255)           tACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTg                                                                                                                            >  2:1040659/1‑140 (MQ=255)             cTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCg                                                                                                                          >  2:390161/1‑140 (MQ=255)             cTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCg                                                                                                                          >  1:21896/1‑140 (MQ=255)             cTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCg                                                                                                                          >  1:891071/1‑140 (MQ=255)             cTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCg                                                                                                                          <  1:104976/140‑1 (MQ=255)               cACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGAc                                                                                                                        <  1:1040659/140‑1 (MQ=255)                  ttCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCtt                                                                                                                     <  2:340991/140‑1 (MQ=255)                       ccACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCgttg                                                                                                                >  2:508014/1‑140 (MQ=255)                         acaAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCgttgtt                                                                                                              <  2:266039/140‑1 (MQ=255)                          caAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGttgttg                                                                                                             <  2:975224/140‑1 (MQ=255)                          caAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGttgttg                                                                                                             >  2:63980/1‑140 (MQ=255)                          caAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGttgttg                                                                                                             <  1:108328/140‑1 (MQ=255)                            aaGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGAc                                                                                                           >  1:399503/1‑140 (MQ=255)                            aaGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGAc                                                                                                           <  1:63980/140‑1 (MQ=255)                             aGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGAcc                                                                                                          <  1:503459/140‑1 (MQ=255)                                aTGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGc                                                                                                       <  1:34257/140‑1 (MQ=255)                                aTGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGc                                                                                                       <  1:886725/140‑1 (MQ=255)                                 tGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCa                                                                                                      <  1:1000617/140‑1 (MQ=255)                                 tGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCa                                                                                                      <  2:1085705/140‑1 (MQ=255)                                     acaGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGt                                                                                                  <  2:118378/140‑1 (MQ=255)                                       agcagcTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTat                                                                                                >  1:552251/1‑140 (MQ=255)                                          agcTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTatcat                                                                                             <  2:891071/140‑1 (MQ=255)                                            ctctGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATcc                                                                                           >  1:214917/1‑140 (MQ=255)                                             tctGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCa                                                                                          <  2:464273/140‑1 (MQ=255)                                                       tCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGaa                                                                                >  2:342323/1‑140 (MQ=255)                                                       tCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGaa                                                                                >  2:267384/1‑140 (MQ=255)                                                           tAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTa                                                                            <  1:332580/140‑1 (MQ=255)                                                                                 ggCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGa                                                      <  2:502536/140‑1 (MQ=255)                                                                                 ggCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGa                                                      <  1:266801/140‑1 (MQ=255)                                                                                 ggCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGa                                                      <  1:491856/140‑1 (MQ=255)                                                                                 ggCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGa                                                      <  1:390161/140‑1 (MQ=255)                                                                                 ggCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGa                                                      <  2:157636/140‑1 (MQ=255)                                                                                            tGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGAcc                                           <  1:508014/140‑1 (MQ=255)                                                                                             ggCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACct                                          >  2:704440/1‑140 (MQ=255)                                                                                               cgcCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACctgc                                        <  1:342323/140‑1 (MQ=255)                                                                                               cgcCCTGACCCGTAACTTCGACAACATGCGTGAAGACGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACctgc                                        >  1:857319/1‑140 (MQ=255)                                                                                                         cGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAAt                              <  2:214917/140‑1 (MQ=255)                                                                                                         cGTAACTTCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAAt                              <  1:704440/140‑1 (MQ=255)                                                                                                              cTTCGACAACATGCGTGAAGACGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAag                         <  2:857319/140‑1 (MQ=255)                                                                                                                tCGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAagca                       >  1:193297/1‑140 (MQ=255)                                                                                                                 cGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAagcag                      >  1:1106903/1‑140 (MQ=255)                                                                                                                 cGACAACATGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAagcag                      >  1:780271/1‑140 (MQ=255)                                                                                                                   acaacaTGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAgcagca                    <  2:399503/140‑1 (MQ=255)                                                                                                                   acaacaTGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAgcagca                    <  2:552251/140‑1 (MQ=255)                                                                                                                        aTGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTa               <  1:11234/140‑1 (MQ=255)                                                                                                                         tGCGTGAAGATGAAGGCCTGGCTGATCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAag                         <  1:653199/129‑1 (MQ=255)                                                                                                                         tGCGTGAAGATGAAGGCCTGGCTGATCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAag                         >  2:653199/1‑129 (MQ=255)                                                                                                                         tGCGTGAAGATGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTAc              >  1:665786/1‑140 (MQ=255)                                                                                                                                  aTGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAgcagca                    >  2:397456/1‑125 (MQ=255)                                                                                                                                  aTGAAGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAgcagca                    <  1:397456/125‑1 (MQ=255)                                                                                                                                     aaGGCCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTAGCTTCTCAc  >  2:465964/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCGACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGTCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTAGCTTCTCAC  >  NC_000913/639156‑639427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑