Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,118	A→G	noncoding (16/77 nt)	metT ←	tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,118	0	A	G	100.0%	160.3 / NA	44	noncoding (16/77 nt)	metT	tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/18);  total (26/18)

GAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAA  >  NC_000913/696983‑697254                                                                                                                                        |                                                                                                                                         gAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGa                                                                                                                                      >  1:162187/1‑140 (MQ=255)  aaCTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAg                                                                                                                                     <  1:924436/140‑1 (MQ=255)             cTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAg                                                                                                                                     >  1:1101200/1‑129 (MQ=255)             cTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAg                                                                                                                                     <  2:1101200/129‑1 (MQ=255)               tGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAg                                                                                                                                     >  1:933523/1‑127 (MQ=255)               tGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAg                                                                                                                                     <  2:933523/127‑1 (MQ=255)               tGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATc                                                                                                                        >  2:221055/1‑140 (MQ=255)                 cggcgCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCtt                                                                                                                      >  2:950013/1‑140 (MQ=255)                         gAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTcg                                                                                                              <  2:162187/140‑1 (MQ=255)                            ccTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGa                                                                                                           <  2:576850/140‑1 (MQ=255)                            ccTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGa                                                                                                           <  2:494010/140‑1 (MQ=255)                            ccTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGa                                                                                                           <  2:968971/140‑1 (MQ=255)                                           tCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGtt                                                                                            >  1:617781/1‑140 (MQ=255)                                           tCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGtt                                                                                            >  1:869488/1‑140 (MQ=255)                                            cTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTg                                                                                           >  1:917359/1‑140 (MQ=255)                                                       gCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCAtt                                                                                >  2:618435/1‑140 (MQ=255)                                                        ccACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTa                                                                               >  2:634831/1‑140 (MQ=255)                                                         cACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAt                                                                              <  1:1047161/140‑1 (MQ=255)                                                               cGCAAAAAAAGATGGTGGCTACGACGGGATTCGAA‑CTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAt                                                                              >  1:759167/1‑133 (MQ=255)                                                               cGCAAAAAAAGATGGTGGCTACGACGGGATTCGAA‑CTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAt                                                                              <  2:759167/133‑1 (MQ=255)                                                                              tggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGt                                                         >  2:147020/1‑140 (MQ=255)                                                                                     gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACctc                                                  >  1:663978/1‑140 (MQ=255)                                                                                         ggATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCtttt                                              <  1:221055/140‑1 (MQ=255)                                                                                                     gtgACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAAtt                                  >  2:465691/1‑140 (MQ=255)                                                                                                      tgACCCCATCATTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTg                                 >  1:767933/1‑140 (MQ=255)                                                                                                             atcatTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGaaa                          >  1:729274/1‑140 (MQ=255)                                                                                                             atcatTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGaaa                          >  2:1006031/1‑140 (MQ=255)                                                                                                             atcatTATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAg                         <  2:334168/140‑1 (MQ=255)                                                                                                                 ttATGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGa                      <  2:617781/140‑1 (MQ=255)                                                                                                                    tGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGt                        <  2:774836/135‑1 (MQ=255)                                                                                                                    tGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGt                        >  1:774836/1‑135 (MQ=255)                                                                                                                    tGAGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTg                   <  1:465691/140‑1 (MQ=255)                                                                                                                      aGTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGtt                 >  1:631472/1‑140 (MQ=255)                                                                                                                       gTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATTCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGa                      <  2:979078/134‑1 (MQ=255)                                                                                                                       gTGATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATTCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGa                      >  1:979078/1‑134 (MQ=255)                                                                                                                         gATGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTgg              >  2:492230/1‑140 (MQ=255)                                                                                                                          aTGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGt             >  1:651508/1‑140 (MQ=255)                                                                                                                          aTGTGCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGt             >  1:108531/1‑140 (MQ=255)                                                                                                                           tgtgCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGtt            <  2:939704/140‑1 (MQ=255)                                                                                                                           tgtgCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGtt            <  1:255158/140‑1 (MQ=255)                                                                                                                             tgCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAg          >  2:244286/1‑140 (MQ=255)                                                                                                                              gCTCTAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAgg         >  1:78788/1‑140 (MQ=255)                                                                                                                                  tAACCAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGc     >  2:976454/1‑140 (MQ=255)                                                                                                                                     ccAGCTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGaa  <  2:663978/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAA  >  NC_000913/696983‑697254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑