Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,008,117	G→A	A92T (GCT→ACT)	rlmL →	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,008,117	0	G	A	100.0%	210.8 / NA	60	A92T (GCT→ACT)	rlmL	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/26);  total (34/26)

AGAGCCTGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGA  >  NC_000913/1007980‑1008250                                                                                                                                          |                                                                                                                                      agagCCTGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACt                                                                                                                                     >  1:485354/1‑140 (MQ=255)    gCCTGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTc                                                                                                                                  <  2:273676/140‑1 (MQ=255)    gCCTGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTc                                                                                                                                  >  2:533330/1‑140 (MQ=255)    gCCTGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTc                                                                                                                                  >  2:326828/1‑140 (MQ=255)       tGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCAc                                                                                                                               >  1:784168/1‑140 (MQ=255)           gtgGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCa                                                                                                                           <  2:838486/140‑1 (MQ=255)               aGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCa                                                                                                                                >  2:32872/1‑131 (MQ=255)               aGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCa                                                                                                                                <  1:32872/131‑1 (MQ=255)                gccgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGt                                                                                                                      <  2:465442/140‑1 (MQ=255)                gccgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGt                                                                                                                      <  2:139881/140‑1 (MQ=255)                gccgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGt                                                                                                                      <  2:831747/140‑1 (MQ=255)                gccgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGt                                                                                                                      <  2:609736/140‑1 (MQ=255)                 ccgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGtt                                                                                                                     >  1:1135588/1‑140 (MQ=255)                   gccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTg                                                                                                                   >  2:653096/1‑140 (MQ=255)                    ccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTc                                                                                                                                  >  1:7213/1‑124 (MQ=255)                    ccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTc                                                                                                                                  <  2:7213/124‑1 (MQ=255)                    ccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTg                                                                                                                        <  2:506387/134‑1 (MQ=255)                    ccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTg                                                                                                                        >  1:506387/1‑134 (MQ=255)                         ccTCGCGTATTATGTTGCCGCTGGGCGAGCGCAAGGCTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGac                                                                                                             >  1:1149668/1‑140 (MQ=255)                            cgcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACAcc                                                                                                          <  2:694705/140‑1 (MQ=255)                              cgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCAt                                                                                                        >  1:376313/1‑140 (MQ=255)                                   tatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCa                                                                                                   <  2:485354/140‑1 (MQ=255)                                   tatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCa                                                                                                   <  2:376313/140‑1 (MQ=255)                                    atGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCaa                                                                                                  <  2:1135588/140‑1 (MQ=255)                                           cGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAAcagtcag                                                                                           >  2:247123/1‑140 (MQ=255)                                               gggCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAAcagtcag                                                                                           >  1:305036/1‑136 (MQ=255)                                               gggCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAAcagtcag                                                                                           <  2:305036/136‑1 (MQ=255)                                               gggCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACg                                                                                       >  1:552046/1‑140 (MQ=255)                                                     gtgtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGa                                                                                 <  1:247123/140‑1 (MQ=255)                                                               tACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaa                                                                       >  1:1005423/1‑140 (MQ=255)                                                                aCAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaa                                                                      >  1:703796/1‑140 (MQ=255)                                                                    cGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAcg                                                                  <  1:59144/140‑1 (MQ=255)                                                                         tAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtc                                                             >  2:598684/1‑140 (MQ=255)                                                                            aCCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtc                                                          >  2:181226/1‑140 (MQ=255)                                                                                ctATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaa                                                                      <  2:1049550/124‑1 (MQ=255)                                                                                ctATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaa                                                                      >  1:1049550/1‑124 (MQ=255)                                                                                ctATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATg                                                      >  2:970037/1‑140 (MQ=255)                                                                                        gtgtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAcg                                              <  1:533330/140‑1 (MQ=255)                                                                                        gtgtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAcg                                              <  1:326828/140‑1 (MQ=255)                                                                                            tCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGa                                          <  1:376563/140‑1 (MQ=255)                                                                                             cAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaa                                         >  1:89463/1‑140 (MQ=255)                                                                                             cAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaa                                         >  1:1020930/1‑140 (MQ=255)                                                                                                      aaCTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGcc                                <  1:598684/140‑1 (MQ=255)                                                                                                      aaCTGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGcc                                <  1:653096/140‑1 (MQ=255)                                                                                                         tGGACAGAGATGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcgcg                             <  1:970037/140‑1 (MQ=255)                                                                                                                agaTGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTCTCACGCGGAAAAATCTGCCGCGTCCAAAt                      >  1:844463/1‑140 (MQ=255)                                                                                                                  aTGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGt                    >  1:1018808/1‑140 (MQ=255)                                                                                                                  aTGTTTAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGt                    >  1:406316/1‑140 (MQ=255)                                                                                                                     tttAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGa                 >  1:836158/1‑140 (MQ=255)                                                                                                                      ttAATCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAt                >  1:913878/1‑140 (MQ=255)                                                                                                                          tCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgcg            >  2:47673/1‑140 (MQ=255)                                                                                                                          tCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgcg            <  2:406316/140‑1 (MQ=255)                                                                                                                          tCCTGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgcg            >  1:178127/1‑140 (MQ=255)                                                                                                                             tGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATg         <  2:552046/140‑1 (MQ=255)                                                                                                                             tGGCGCGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATg         >  1:541028/1‑140 (MQ=255)                                                                                                                               gcgcGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATgcg       >  1:430629/1‑140 (MQ=255)                                                                                                                               gcgcGACCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATgcg       >  2:179450/1‑140 (MQ=255)                                                                                                                                    aCCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGCAAAATCTGCCGCGTCCAAATGTTGATCGCGATgc        <  2:1025730/134‑1 (MQ=255)                                                                                                                                    aCCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATgc        >  1:1025730/1‑134 (MQ=255)                                                                                                                                    aCCTTCACTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGa  >  1:472907/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AGAGCCTGATGTGGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGA  >  NC_000913/1007980‑1008250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑