Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,609	A→G	intergenic (+72/‑8)	appX → / → appA	small protein AppX/periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,609	0	A	G	100.0%	206.2 / NA	57	intergenic (+72/‑8)	appX/appA	small protein AppX/periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/22);  total (35/22)

TGTTGATGTGTTCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAACATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTGTGCGTGC  >  NC_000913/1040476‑1040744                                                                                                                                      |                                                                                                                                        tgttgATGTGTTCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGGTAACGAACGTAGGCCTGGTGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATc                                                                                                                                   >  2:75282/1‑140 (MQ=255)         tgtTCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAg                                                                                                                           <  2:383272/140‑1 (MQ=255)         tgtTCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAg                                                                                                                           <  2:379158/140‑1 (MQ=255)           ttCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAg                                                                                                                           <  1:1052643/138‑1 (MQ=255)           ttCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAg                                                                                                                           >  2:1052643/1‑138 (MQ=255)                     aCCCTTGTGTTGGTATGGCTGAACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGc                                                                                                                          <  2:372059/129‑1 (MQ=255)                     aCCCTTGTGTTGGTATGGCTGAACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGc                                                                                                                          >  1:372059/1‑129 (MQ=255)                          tgtgTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCAttt                                                                                                          >  2:583155/1‑140 (MQ=255)                                 gTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTAtctc                                                                                                   >  2:715265/1‑140 (MQ=255)                                 gTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTAtctc                                                                                                   >  1:135469/1‑140 (MQ=255)                                 gTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCCGAAGCATATCGATGAAAGCGATCTTAATACCATTTTTAtctc                                                                                                   >  1:76331/1‑140 (MQ=255)                                      gCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTg                                                                                              >  2:869587/1‑140 (MQ=255)                                        tGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGAt                                                                                            <  1:583155/140‑1 (MQ=255)                                          gACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTAtc                                                                                                     <  2:482451/129‑1 (MQ=255)                                          gACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTAtc                                                                                                     >  1:482451/1‑129 (MQ=255)                                           aCCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTcc                                                                                         >  2:1101545/1‑140 (MQ=255)                                           aCCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTcc                                                                                         >  2:508981/1‑140 (MQ=255)                                                cgTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTaa                                                                                    >  2:705847/1‑140 (MQ=255)                                                cgTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTaa                                                                                    >  1:1111381/1‑140 (MQ=255)                                                cgTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTaa                                                                                    >  2:526912/1‑140 (MQ=255)                                                cgTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTaa                                                                                    >  1:45211/1‑140 (MQ=255)                                                     gAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCg                                                                               <  1:508981/140‑1 (MQ=255)                                                           ttAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCg                                                                               <  1:693946/134‑1 (MQ=255)                                                           ttAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCg                                                                               >  2:693946/1‑134 (MQ=255)                                                           ttAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCt                                                                         >  2:805161/1‑140 (MQ=255)                                                              acgaacgTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCa                                                                      <  2:135469/140‑1 (MQ=255)                                                              acgaacgTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCa                                                                      <  2:1001091/140‑1 (MQ=255)                                                              acgaacgTAGGCCTGATGCGGCGCATTAGCATCGCATCAGACAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCa                                                                      <  2:655933/140‑1 (MQ=255)                                                                    gTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCt                                                                <  1:75282/140‑1 (MQ=255)                                                                     tAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTc                                                               <  1:106366/140‑1 (MQ=255)                                                                         ccTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCagag                                                           <  1:526912/140‑1 (MQ=255)                                                                             aTGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAg                                                       >  2:399304/1‑140 (MQ=255)                                                                              tGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGc                                                      >  1:33176/1‑140 (MQ=255)                                                                              tGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGc                                                      >  2:274758/1‑140 (MQ=255)                                                                                  gcgcATTAGCATCGCATCAGGCAATCAATAACGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGa                                                  >  2:550032/1‑140 (MQ=255)                                                                                      aTTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTg                                              <  2:1111381/140‑1 (MQ=255)                                                                                        tAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGaa                                            <  1:805161/140‑1 (MQ=255)                                                                                              cgcatcAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGa                                      <  1:869587/140‑1 (MQ=255)                                                                                               gcatcAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGaa                                     >  1:1060995/1‑140 (MQ=255)                                                                                                catcAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGaaa                                    >  2:561859/1‑140 (MQ=255)                                                                                                 atcAGGCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAg                                   <  1:1101545/140‑1 (MQ=255)                                                                                                     ggCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGtgtg                               <  1:300862/140‑1 (MQ=255)                                                                                                     ggCAATCAATAATGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGtgtg                               >  2:300862/1‑140 (MQ=255)                                                                                                               aatGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCa                     >  1:611283/1‑140 (MQ=255)                                                                                                                atGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAg                    <  1:1111389/140‑1 (MQ=255)                                                                                                                atGTCAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAg                    >  1:996622/1‑140 (MQ=255)                                                                                                                    cAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAgtcgt                >  2:1085997/1‑140 (MQ=255)                                                                                                                    cAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAgtcgt                >  1:635543/1‑140 (MQ=255)                                                                                                                    cAGATATGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAgtcgt                >  1:392724/1‑140 (MQ=255)                                                                                                                         atGAAAAGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATgg           <  1:992816/140‑1 (MQ=255)                                                                                                                              aaGCGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgc      <  1:274758/140‑1 (MQ=255)                                                                                                                                 cGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgcgtg   >  1:101885/1‑140 (MQ=255)                                                                                                                                 cGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgcgtg   >  2:876005/1‑140 (MQ=255)                                                                                                                                 cGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgcgtg   >  1:961519/1‑140 (MQ=255)                                                                                                                                 cGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgcgtg   >  1:703523/1‑140 (MQ=255)                                                                                                                                 cGGAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgcgtg   >  1:436661/1‑140 (MQ=255)                                                                                                                                  ggAAGCATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTgtgcgtgc  >  1:259941/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TGTTGATGTGTTCGCTCTCCACCCTTGTGTTGGTATGGCTGGACCCGCGTCTGAAAAGTTAACGAACGTAGGCCTGATGCGGCGCATTAGCATCGCATCAGGCAATCAATAATGTCAGATATGAAAAGCGGAAACATATCGATGAAAGCGATCTTAATCCCATTTTTATCTCTTCTGATTCCGTTAACCCCGCAATCTGCATTCGCTCAGAGTGAGCCGGAGCTGAAGCTGGAAAGTGTGGTGATTGTCAGTCGTCATGGTGTGCGTGC  >  NC_000913/1040476‑1040744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑