Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,067,364	A→G	G115G (GGT→GGC)	wrbA ←	NAD(P)H:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,067,364	0	A	G	100.0%	177.4 / NA	49	G115G (GGT→GGC)	wrbA	NAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

GTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTACCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTACCAAAAATAATGGC  >  NC_000913/1067229‑1067498                                                                                                                                        |                                                                                                                                       gTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCgg                                                                                                                                    <  2:863664/140‑1 (MQ=255) gTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCgg                                                                                                                                    <  1:334026/140‑1 (MQ=255) gTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCgg                                                                                                                                    <  1:750498/140‑1 (MQ=255)  tACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGt                                                                                                                                   <  1:1096278/140‑1 (MQ=255)      gCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGaa                                                                                                                               <  1:684590/140‑1 (MQ=255)              ccgcGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGt                                                                                                                                   >  2:953856/1‑128 (MQ=255)              ccgcGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGt                                                                                                                                   <  1:953856/128‑1 (MQ=255)                    aCCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTTATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGc                                                                                                                 >  1:424212/1‑140 (MQ=255)                      cTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCa                                                                                                               <  2:952862/140‑1 (MQ=255)                        gtgAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGt                                                                                                             <  2:803512/140‑1 (MQ=255)                        gtgAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGt                                                                                                             <  2:335621/140‑1 (MQ=255)                                tCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGta                                                                                                     >  2:1083160/1‑140 (MQ=255)                                     taatTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAGGACGCTCGCCAGTTTTCCGTATAGTg                                                                                                <  1:560391/140‑1 (MQ=255)                                         tCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGcc                                                                                            >  1:168030/1‑140 (MQ=255)                                            tgcgctgcgTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGa                                                                                         >  1:42984/1‑140 (MQ=255)                                              cgctgcgTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAg                                                                                       >  1:1023162/1‑140 (MQ=255)                                                   cgTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCcac                                                                                  >  2:170996/1‑140 (MQ=255)                                                             aGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCg                                                                        >  2:172624/1‑140 (MQ=255)                                                              gggACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGt                                                                       <  2:42984/140‑1 (MQ=255)                                                              gggACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGt                                                                       <  2:168030/140‑1 (MQ=255)                                                               ggACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTc                                                                      <  1:824949/140‑1 (MQ=255)                                                                  cAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTgg                                                                   >  1:734111/1‑140 (MQ=255)                                                                   aaTTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGt                                                                  <  1:1067911/140‑1 (MQ=255)                                                                       aCCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAg                                                              <  1:1083160/140‑1 (MQ=255)                                                                         cATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTTATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGa                                                            <  2:424212/140‑1 (MQ=255)                                                                         cATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGa                                                            <  1:398688/140‑1 (MQ=255)                                                                             ccGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGt                                                        >  1:935219/1‑140 (MQ=255)                                                                             ccGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGt                                                        >  2:734613/1‑140 (MQ=255)                                                                              cGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGCGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTa                                                       >  1:627066/1‑140 (MQ=255)                                                                               gTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTAc                                                      >  2:411755/1‑140 (MQ=255)                                                                                agATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACg                                                     >  2:1090851/2‑140 (MQ=255)                                                                                  aTGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCa                                                   >  1:327484/1‑140 (MQ=255)                                                                                       cAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTa                                                       <  2:32449/131‑1 (MQ=255)                                                                                       cAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTa                                                       >  1:32449/1‑131 (MQ=255)                                                                                            gtcgtcCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGa                                         >  2:847678/1‑140 (MQ=255)                                                                                             tcgtcCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCa                                                   <  1:892469/129‑1 (MQ=255)                                                                                             tcgtcCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCa                                                   >  2:892469/1‑129 (MQ=255)                                                                                               gtcCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACAt                                      >  2:883059/1‑140 (MQ=255)                                                                                                      tGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCa                               <  2:134181/140‑1 (MQ=255)                                                                                                         aTGTGATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAg                            <  2:1023162/140‑1 (MQ=255)                                                                                                             gATGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCggg                        <  2:935219/140‑1 (MQ=255)                                                                                                               tGGTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTa                      <  1:170996/140‑1 (MQ=255)                                                                                                                ggTTTGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAg                     <  1:847678/140‑1 (MQ=255)                                                                                                                  tttGTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGgt                   >  2:828979/1‑140 (MQ=255)                                                                                                                     gTTCCTGACCGCCGCCAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTa                <  2:627066/140‑1 (MQ=255)                                                                                                                                ccgccAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTACCAAAaataat     >  2:121310/1‑140 (MQ=255)                                                                                                                                 cgccAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTACCAAAAATAATg    >  2:168366/1‑140 (MQ=255)                                                                                                                                 cgccAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTACCAAAAATAATg    >  2:15664/1‑140 (MQ=255)                                                                                                                                   ccAGTGCCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTACCAAAAATAATGGc  >  1:894252/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTGCGTAGCCAATAGGGACAATTACCATGCCGTGATGCGCAAGGGTCGTCCAGGTGGATGTGATGGTTTGTTCCTGACCGCCGCCAGTACCGGTGGAACTAAAGACGCTCGCCAGTTTTCCGTATAGTGCGCCGGAAGCCCACAGGCCGCCCGTCTGGTCGAGGAAGGTACGCATTTGACCGGACATGTTGCCAAAGCGGGTAGGTGTACCAAAAATAATGGC  >  NC_000913/1067229‑1067498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑