Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,105,562	C→T	P50L (CCC→CTC)	ymdA →	uncharacterized protein YmdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,105,562	0	C	T	100.0%	146.0 / NA	41	P50L (CCC→CTC)	ymdA	uncharacterized protein YmdA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

TCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGC  >  NC_000913/1105437‑1105696                                                                                                                              |                                                                                                                                       tctTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACt                                                                                                                          >  2:935958/1‑140 (MQ=255)    tATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGcc                                                                                                                       >  1:767779/1‑140 (MQ=255)      tGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCca                                                                                                                     <  1:227446/140‑1 (MQ=255)           ggacgtttttttTTTCCTTTAACTGACATTGCTCGAAGGACCGTGCGAGGGTGCGTGATCCATTTTTATGGCCTAATTGTGGAACCGGAATGTGAAGTCAGAACCTAGTCATCACTCGTAGAAATGATCTGCCCACAgta                                                                                                                <  2:767779/136‑3 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  2:435009/140‑1 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  1:934926/140‑1 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                >  2:1140860/1‑140 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                >  2:244669/1‑140 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                >  2:269337/1‑140 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  2:304644/140‑1 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  2:772712/140‑1 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  1:269337/140‑1 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  1:1140860/140‑1 (MQ=255)           ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGaa                                                                                                                <  1:102727/140‑1 (MQ=255)                tttgtttTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGtt                                                                                                           >  2:237488/1‑140 (MQ=255)                      ttttCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTc                                                                                                     <  1:1157118/140‑1 (MQ=255)                          ccTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCggg                                                                                                 <  1:244669/140‑1 (MQ=255)                           cTTTTATTGCCATTGTTGGAAGCCCCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGc                                                                                                <  2:418789/140‑1 (MQ=255)                              ttATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCaaa                                                                                             >  2:1076650/1‑140 (MQ=255)                                 ttGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAAcc                                                                                          <  1:1076650/140‑1 (MQ=255)                                     cATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCGCCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACt                                                                                      <  1:616131/140‑1 (MQ=255)                                                    cGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTaa                                                                       >  1:758266/1‑140 (MQ=255)                                                       gCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGa                                                                    >  1:574622/1‑140 (MQ=255)                                                              ggCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAg                                                                   <  1:88622/134‑1 (MQ=255)                                                              ggCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAg                                                                   >  2:88622/1‑134 (MQ=255)                                                                cGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATg                                                           >  2:376558/1‑140 (MQ=255)                                                                               tGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCaaa                                            <  1:205499/140‑1 (MQ=255)                                                                                 gCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATa                                          >  2:407474/1‑140 (MQ=255)                                                                                   cAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGc                                        <  1:376558/140‑1 (MQ=255)                                                                                   cAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGc                                        >  2:662603/1‑140 (MQ=255)                                                                                      aTTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTc                                     >  2:994715/1‑140 (MQ=255)                                                                                           ggAACCGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTaa                                <  1:463996/140‑1 (MQ=255)                                                                                               ccGGCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGt                            >  1:575471/1‑140 (MQ=255)                                                                                                 ggCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGc                          >  2:596995/1‑140 (MQ=255)                                                                                                 ggCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGc                          >  2:991723/1‑140 (MQ=255)                                                                                                  gCATGTGACGTCAGCACCCAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCa                         <  1:662603/140‑1 (MQ=255)                                                                                                                  cccAGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAg         <  1:596995/140‑1 (MQ=255)                                                                                                                     aGTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGaaa      >  1:461987/1‑140 (MQ=255)                                                                                                                      gTCATCACTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGaaaa     >  1:928054/1‑140 (MQ=255)                                                                                                                       tcatcaCTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGaaaaa    <  2:575471/140‑1 (MQ=255)                                                                                                                        catcaCTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAg   <  2:461987/140‑1 (MQ=255)                                                                                                                         atcaCTCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAgc  >  1:293766/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       TCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGC  >  NC_000913/1105437‑1105696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑