Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,122,056	A→G	A184A (GCT→GCC)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,122,056	0	A	G	100.0%	216.3 / NA	60	A184A (GCT→GCC)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/29);  total (31/29)

ATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGC  >  NC_000913/1121921‑1122191                                                                                                                                        |                                                                                                                                        atTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCAt                                                                                                                                     <  2:231351/140‑1 (MQ=255)    aCGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCtt                                                                                                                                  <  1:832483/140‑1 (MQ=255)          gAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTtggtgg                                                                                                                            >  1:468365/1‑140 (MQ=255)                 ggTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGAtgtg                                                                                                                     >  2:306165/1‑140 (MQ=255)                 ggTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGAtgtg                                                                                                                     <  1:673193/140‑1 (MQ=255)                  gTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGc                                                                                                                    >  2:87509/1‑140 (MQ=255)                          tACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAAc                                                                                                            >  1:1008594/1‑140 (MQ=255)                               gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACtt                                                                                                       >  2:748864/1‑140 (MQ=255)                               gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACtt                                                                                                       >  2:494413/1‑140 (MQ=255)                               gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACtt                                                                                                       >  1:1089309/1‑140 (MQ=255)                               gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACACAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACtt                                                                                                       >  2:329668/1‑140 (MQ=255)                                tGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACttt                                                                                                      >  2:710703/1‑140 (MQ=255)                                tGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACttt                                                                                                      >  2:76173/1‑140 (MQ=255)                                  cGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCa                                                                                                    >  2:764339/1‑140 (MQ=255)                                  cGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCa                                                                                                    >  1:819138/1‑140 (MQ=255)                                   ggACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAg                                                                                                   <  1:710703/140‑1 (MQ=255)                                         acgcCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGt                                                                                             >  2:247541/1‑140 (MQ=255)                                                  aCCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTg                                                                                    >  2:797429/1‑140 (MQ=255)                                                       cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCa                                                                               >  1:133475/1‑140 (MQ=255)                                                       cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCa                                                                               >  2:928889/1‑140 (MQ=255)                                                       cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATAGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCa                                                                               >  2:130064/1‑140 (MQ=255)                                                         tatGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCaga                                                                             >  1:1093724/1‑140 (MQ=255)                                                           tGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAgg                                                                           <  1:1026242/140‑1 (MQ=255)                                                             gTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGtt                                                                         <  1:748864/140‑1 (MQ=255)                                                                 cGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCAt                                                                     >  2:941621/1‑140 (MQ=255)                                                                 cGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCAt                                                                     >  1:295298/1‑140 (MQ=255)                                                                  ggTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATc                                                                    >  2:658586/1‑140 (MQ=255)                                                                   gTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCa                                                                   <  1:87509/140‑1 (MQ=255)                                                                   gTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCa                                                                   <  2:819138/140‑1 (MQ=255)                                                                    ttAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCAc                                                                  <  2:295298/140‑1 (MQ=255)                                                                      aaaCATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGc                                                                <  1:797429/140‑1 (MQ=255)                                                                      aaaCATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGc                                                                <  2:1093724/140‑1 (MQ=255)                                                                      aaaCATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGc                                                                <  1:910286/140‑1 (MQ=255)                                                                              gATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCtata                                                        >  1:954317/1‑140 (MQ=255)                                                                              gATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCtata                                                        >  1:811940/1‑140 (MQ=255)                                                                              gATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCtata                                                        >  1:925134/1‑140 (MQ=255)                                                                              gATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCtata                                                        >  1:82994/1‑140 (MQ=255)                                                                                tgctgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATaaa                                                      <  1:76173/140‑1 (MQ=255)                                                                                   tgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAgcg                                                   <  1:361887/140‑1 (MQ=255)                                                                                    gcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAgcgc                                                  >  2:1091608/1‑140 (MQ=255)                                                                                         gTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTc                                             <  2:1008594/140‑1 (MQ=255)                                                                                          tGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCa                                            <  1:764339/140‑1 (MQ=255)                                                                                               gtggCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAg                                                     >  2:602898/1‑126 (MQ=255)                                                                                               gtggCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAg                                                     <  1:602898/126‑1 (MQ=255)                                                                                                 ggCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCaa                                     <  2:954317/140‑1 (MQ=255)                                                                                                   cagccagcCGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGCTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCaaaa                                   <  2:1035629/140‑1 (MQ=255)                                                                                                      ccagccGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATg                                >  2:619594/1‑140 (MQ=255)                                                                                                          ccGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCg                             <  2:966256/139‑1 (MQ=255)                                                                                                          ccGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCg                             >  1:966256/1‑139 (MQ=255)                                                                                                           cGTTCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGat                           <  1:928889/140‑1 (MQ=255)                                                                                                             ttCATTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGatat                         <  2:925134/140‑1 (MQ=255)                                                                                                                aTTTCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTg                      <  2:624025/140‑1 (MQ=255)                                                                                                                  ttCCGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTTACGATCAAAAATGTCGATATCTGCa                    <  2:1089309/140‑1 (MQ=255)                                                                                                                    ccGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCAtg                  <  1:306165/140‑1 (MQ=255)                                                                                                                     cGTCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCAtgt                 >  2:726598/1‑140 (MQ=255)                                                                                                                       tCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGatat                         <  2:647628/130‑1 (MQ=255)                                                                                                                       tCACGGACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGatat                         >  1:647628/1‑130 (MQ=255)                                                                                                                           ggACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACt           <  2:133475/140‑1 (MQ=255)                                                                                                                            gACATAGTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACtt          <  1:1082731/140‑1 (MQ=255)                                                                                                                                  gTCGGCGGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCAt    <  1:329668/140‑1 (MQ=255)                                                                                                                                    cggcggCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGc  <  1:1112336/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGC  >  NC_000913/1121921‑1122191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑