Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	62,766	G→A	R167C (CGC→TGC)	rapA ←	RNA polymerase‑binding ATPase and RNAP recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	62,766	0	G	A	100.0%	168.5 / NA	48	R167C (CGC→TGC)	rapA	RNA polymerase‑binding ATPase and RNAP recycling factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/25);  total (23/25)

TGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGG  >  NC_000913/62630‑62893                                                                                                                                         |                                                                                                                                tGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAAcc                                                                                                                              <  2:620325/140‑1 (MQ=255) tGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAAcc                                                                                                                              <  2:809407/140‑1 (MQ=255)   tAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCaa                                                                                                                            >  2:637998/1‑140 (MQ=255)   tAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCaa                                                                                                                            <  1:765882/140‑1 (MQ=255)     aGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAAca                                                                                                                          >  1:123749/1‑140 (MQ=255)           ccGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGa                                                                                                                    >  2:352127/1‑140 (MQ=255)            cGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAg                                                                                                                   <  1:949493/140‑1 (MQ=255)             gggACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGc                                                                                                                  >  2:1040273/1‑140 (MQ=255)                         aGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTg                                                                                                      >  1:357976/1‑140 (MQ=255)                           cacaCGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGAt                                                                                                    >  1:64267/1‑140 (MQ=255)                           cacaCGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGAt                                                                                                    >  1:395890/1‑140 (MQ=255)                            acacGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATg                                                                                                   <  1:302464/140‑1 (MQ=255)                            acacGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATg                                                                                                   >  2:1101608/1‑140 (MQ=255)                                 ttCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGa                                                                                              <  2:395890/140‑1 (MQ=255)                                  tCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGAt                                                                                             <  2:123749/140‑1 (MQ=255)                                    agcagcGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCa                                                                                           <  2:64267/140‑1 (MQ=255)                                        gcgcCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCt                                                                                       >  1:67869/1‑140 (MQ=255)                                        gcgcCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCt                                                                                       <  2:129972/140‑1 (MQ=255)                                        gcgcCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCt                                                                                       <  1:1101608/140‑1 (MQ=255)                                         cgcCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTg                                                                                      >  1:197149/1‑140 (MQ=255)                                                gagCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCt                                                                               >  1:150388/1‑140 (MQ=255)                                                      ttGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACg                                                                                 <  1:652912/132‑1 (MQ=255)                                                      ttGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACg                                                                                 >  2:652912/1‑132 (MQ=255)                                                      ttGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACcgc                                                                         >  2:129534/1‑140 (MQ=255)                                                              gCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCg                                                                 <  2:67869/140‑1 (MQ=255)                                                                   atcatcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTa                                                            <  2:436692/140‑1 (MQ=255)                                                                   atcatcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTa                                                            <  1:1040273/140‑1 (MQ=255)                                                                     catcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACg                                                          <  1:637998/140‑1 (MQ=255)                                                                     catcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACg                                                          <  2:150388/140‑1 (MQ=255)                                                                       tcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGTTGGTACGCTGACCGCGCAGGCCGCTGTACGGc                                                        >  1:663637/1‑140 (MQ=255)                                                                       tcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGc                                                        >  2:199333/1‑140 (MQ=255)                                                                       tcCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGc                                                        >  1:128222/1‑140 (MQ=255)                                                                            gCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTa                                                            <  2:627374/131‑1 (MQ=255)                                                                            gCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTa                                                            >  1:627374/1‑131 (MQ=255)                                                                                 aaTGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACt                                              >  1:155568/1‑140 (MQ=255)                                                                                  aTGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTg                                             >  2:566291/1‑140 (MQ=255)                                                                                                    aCTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTAcg                           >  2:556907/1‑140 (MQ=255)                                                                                                      ttCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTAcgcg                         <  2:991189/140‑1 (MQ=255)                                                                                                                agcagGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACg               <  2:197149/140‑1 (MQ=255)                                                                                                                  cagGACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCa             >  2:705726/1‑140 (MQ=255)                                                                                                                     gACGCGCGGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAgcg          <  1:566291/140‑1 (MQ=255)                                                                                                                       cgcgcgGCGCGTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCa        <  1:352127/140‑1 (MQ=255)                                                                                                                         cgcggcgcgTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACg               <  2:176450/131‑1 (MQ=255)                                                                                                                         cgcggcgcgTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACg               >  1:176450/1‑131 (MQ=255)                                                                                                                           cggcgcgTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGc    <  1:199333/140‑1 (MQ=255)                                                                                                                             gcgcgTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCgg  <  1:804729/140‑1 (MQ=255)                                                                                                                                cgTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCg   <  2:1008568/136‑1 (MQ=255)                                                                                                                                cgTGGCGGCAACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCg   >  1:1008568/1‑136 (MQ=255)                                                                                                                                         |                                                                                                                                TGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTACGCTGACCGCGCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGG  >  NC_000913/62630‑62893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑