Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,173,437	A→G	S176S (AGT→AGC)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,173,437	0	A	G	100.0%	169.2 / NA	47	S176S (AGT→AGC)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/28);  total (19/28)

GCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCACTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATGCATCTCGTGACAGGCGCTGACCTTTT  >  NC_000913/1173300‑1173572                                                                                                                                          |                                                                                                                                        gCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCt                                                                                                                                       >  1:652828/1‑140 (MQ=255) gCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCt                                                                                                                                       >  1:262727/1‑140 (MQ=255)   tttATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTcc                                                                                                                                     <  2:1117083/140‑1 (MQ=255)   tttATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTcc                                                                                                                                     <  2:433442/140‑1 (MQ=255)   tttATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTcc                                                                                                                                     <  2:442095/140‑1 (MQ=255)       tcggtcggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCAt                                                                                                                                 <  2:652828/140‑1 (MQ=255)        cggtcggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATc                                                                                                                                <  1:346623/140‑1 (MQ=255)         ggtcggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCg                                                                                                                               <  1:1063062/140‑1 (MQ=255)         ggtcggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCg                                                                                                                               >  2:1016044/1‑140 (MQ=255)            ctgAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGa                                                                                                                            >  1:837038/3‑140 (MQ=255)              gAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAag                                                                                                                          <  1:85748/140‑1 (MQ=255)                aaTTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAagag                                                                                                                        <  2:92697/140‑1 (MQ=255)                     gTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCa                                                                                                                   >  1:218405/1‑140 (MQ=255)                     gTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCa                                                                                                                   >  1:840899/1‑140 (MQ=255)                                   gATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAAcgcgccgcgc                                                                                                     <  2:218405/140‑1 (MQ=255)                                      tGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTg                                                                                                  <  2:840899/140‑1 (MQ=255)                                              tCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTc                                                                                          >  1:1600/1‑140 (MQ=255)                                               cTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGCTTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCg                                                                                         <  2:1009504/140‑1 (MQ=255)                                               cTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCg                                                                                         <  2:837038/140‑1 (MQ=255)                                               cTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCg                                                                                         <  1:908315/140‑1 (MQ=255)                                                  cTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCg                                                                                      <  2:552331/140‑1 (MQ=255)                                                     cctccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGt                                                                                     >  2:979420/1‑138 (MQ=255)                                                     cctccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGt                                                                                     <  1:979420/138‑1 (MQ=255)                                                       tccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTc                                                                                 >  2:922153/1‑140 (MQ=255)                                                       tccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTc                                                                                 >  2:182913/1‑140 (MQ=255)                                                       tccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTc                                                                                 >  1:82052/1‑140 (MQ=255)                                                       tccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTc                                                                                 >  1:423196/1‑140 (MQ=255)                                                       tccAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTc                                                                                 >  2:264240/1‑140 (MQ=255)                                                            cGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCa                                                                            >  1:914264/1‑140 (MQ=255)                                                                  ctggctgTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGt                                                                      <  1:182913/140‑1 (MQ=255)                                                                  ctggctgTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGt                                                                      <  2:82052/140‑1 (MQ=255)                                                                      ctgTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAAc                                                                  <  1:922153/140‑1 (MQ=255)                                                                               tCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATa                                                         >  1:493405/1‑140 (MQ=255)                                                                                 aaaCACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAAc                                                       >  2:15286/1‑140 (MQ=255)                                                                                   acacCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCg                                                     >  2:603204/1‑140 (MQ=255)                                                                                   acacCCGCAGGCTGTCGATTCCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCg                                                     <  1:603204/140‑1 (MQ=255)                                                                                      cccGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGgcg                                                  <  2:623996/140‑1 (MQ=255)                                                                                      cccGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGgcg                                                  <  2:914264/140‑1 (MQ=255)                                                                                      cccGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGgcg                                                  <  1:10435/140‑1 (MQ=255)                                                                                         gCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTg                                               <  2:1600/140‑1 (MQ=255)                                                                                              cTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAg                                          <  2:493405/140‑1 (MQ=255)                                                                                                  cGATTTCATCATCAAAGAAATCAAGACGANAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCANCACCTGGTCAACNTGGCGATAACCGGCGCTGTCCAGTTgg                                      <  2:685989/140‑1 (MQ=255)                                                                                                      ttCATCATCAAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCTACATGGCGATAACCGGCGCTGTCCAGTTGGGTTc                                  <  2:1026535/140‑1 (MQ=255)                                                                                                           catcaAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAAt                             >  2:199174/1‑140 (MQ=255)                                                                                                            atcaAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATg                            <  2:262727/140‑1 (MQ=255)                                                                                                             tcaAAGAAATCAAGACGATAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATGc                           <  1:201071/140‑1 (MQ=255)                                                                                                                              aTAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATGCATCTCGTGACAGGCGCt          <  2:423196/140‑1 (MQ=255)                                                                                                                               tAAGGCAGCTCGCTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATGCATCTCGTGACAGGCGCTg         >  1:756442/1‑140 (MQ=255)                                                                                                                                      gctcgctcCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATGCATCTCGTGACAGGCGCTGACCtttt  >  1:233492/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCACTCCCCATCGGGAAGAGATCCAGCAACGCGCCGCGCGTGGCGTATTCGCCGTGCTCCATCACCTGGTCAACATGGCGATAACCGGCGCTGTCCAGTTGGGTTCGTAATGCATCTCGTGACAGGCGCTGACCTTTT  >  NC_000913/1173300‑1173572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑