Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,264,239	A→G	I176V (ATC→GTC)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,264,239	0	A	G	100.0%	107.8 / NA	31	I176V (ATC→GTC)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/24);  total (7/24)

GAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAGATGATTATCGCCAACCGCACTCGCGAACGTGCCCA  >  NC_000913/1264112‑1264372                                                                                                                                |                                                                                                                                      gAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGc                                                                                                                           <  2:539949/140‑1 (MQ=255)    ggCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTTAATCGctct                                                                                                                        >  1:828077/1‑140 (MQ=255)    ggCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGaa                                                                                                                               <  1:117953/133‑1 (MQ=255)    ggCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGaa                                                                                                                               >  2:117953/1‑133 (MQ=255)    ggCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGctc                                                                                                                         >  1:629827/1‑139 (MQ=255)    ggCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGctc                                                                                                                         <  2:629827/139‑1 (MQ=255)     gCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGctctc                                                                                                                       <  2:870373/140‑1 (MQ=255)     gCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGctctc                                                                                                                       >  2:497594/1‑140 (MQ=255)     gCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGctctc                                                                                                                       <  2:170693/140‑1 (MQ=255)        aGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTAc                                                                                                                    <  1:685103/140‑1 (MQ=255)         gCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCCGGGCCTTTAATCGCTCCCTTCg                                                                                                                   >  2:907188/1‑140 (MQ=255)         gCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGGCTTTGAATCGCTCTCTACg                                                                                                                   >  2:240270/1‑140 (MQ=255)          cGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGGCTTTGAATCGCTCTCTACgg                                                                                                                  >  2:35191/1‑140 (MQ=255)             aCTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTca                                                                                                               <  2:889162/140‑1 (MQ=255)               tGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGGGCGGCAGGGCTTTTAATTGCCCTCTACCGTcacc                                                                                                             >  2:837469/1‑139 (MQ=255)               tGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTcaca                                                                                                             <  1:240270/140‑1 (MQ=255)               tGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTCTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTcaca                                                                                                             <  2:1017701/140‑1 (MQ=255)                  aaCGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAgtg                                                                                                          <  1:346664/140‑1 (MQ=255)                           tCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATTGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACCCTGGCGAGGCAGGGGCTTGAATCGCAGTCTTCGGTCACAGGGTTggtcggg                                                                                                 >  2:625844/1‑134 (MQ=255)                               gAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGGGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGgcg                                                                                             <  1:837469/140‑1 (MQ=255)                               gAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGgcg                                                                                             <  1:783498/140‑1 (MQ=255)                                      ttCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGa                                                                                      <  1:190801/140‑1 (MQ=255)                                                cgAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAg                                                                            <  1:497594/140‑1 (MQ=255)                                                   aaCGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCtg                                                                         >  2:1044785/1‑140 (MQ=255)                                                     cgcgTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCtggt                                                                       <  2:671014/140‑1 (MQ=255)                                                              aCTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCa                                                              <  1:120147/140‑1 (MQ=255)                                                                          atCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTGGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAaca                                                  <  2:549577/140‑1 (MQ=255)                                                                          atCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAaca                                                  <  1:907188/140‑1 (MQ=255)                                                                          atCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGGCTTTTAATCGCTCTCTACGGGCACAGGGTTTCTGGGGGGCGCGGGGGAAACCAACGAGCCGGGTGGGCGTCCTCTGcgcggaaa                                                  >  2:321805/1‑136 (MQ=255)                                                                            cGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGTGCGAAcaca                                                <  1:35191/140‑1 (MQ=255)                                                                            cGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAAcaca                                                <  2:951175/140‑1 (MQ=255)                                                                                  cAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTTCGCTGGGGCGGCAGGGCTTTTTATCGCTCTCTACGGGCACAGTGTTGGTGGGAGGCGGGGGGGGAAATATCGGGGTGGGGGCGCGGCCTCTTCGCGGAAACaaaagac                                          >  2:704306/1‑136 (MQ=255)                                                                                  cAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTTAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGGGGAAATATCGAGCTGGTGGGGCGGCATCTGCGCGAACACaaaagac                                          >  2:131343/1‑136 (MQ=255)                                                                                    gcgcTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACag                                        <  1:704306/140‑1 (MQ=255)                                                                                            tctgtcGCTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAgatgat                                <  2:1147871/140‑1 (MQ=255)                                                                                                   cTTTTGCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAGATGATTATCGcc                         <  1:1044785/140‑1 (MQ=255)                                                                                                        gCGGCTTGTACGCTGGCGCGGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAGATGATTATCGCCAACCg                    <  2:420950/140‑1 (MQ=255)                                                                                                                        cgcgGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAGATGATTATCGCCAACCGCACTCGCGAACGTGcc    >  2:1014691/1‑140 (MQ=255)                                                                                                                          cgGCAGGTCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAGATGATTATCGCCAACCGCACTCGCGAACGTGCCCa  >  2:967819/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                      GAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTGAATCGCTCTCTACGGTCACAGTGTTGCTGGTAGGCGCGGGCGAAACTATCGAGCTGGTGGCGCGTCATCTGCGCGAACACAAAGTACAGAAGATGATTATCGCCAACCGCACTCGCGAACGTGCCCA  >  NC_000913/1264112‑1264372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑