Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,484,109	T→C	V350A (GTC→GCC)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,484,109	0	T	C	100.0%	107.6 / NA	30	V350A (GTC→GCC)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

CCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATC  >  NC_000913/1483977‑1484243                                                                                                                                     |                                                                                                                                       ccgccgATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGaa                                                                                                                                 <  1:101093/140‑1 (MQ=255) ccgccgATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGaa                                                                                                                                 <  2:1014738/140‑1 (MQ=255)      gATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTc                                                                                                                            <  2:276769/140‑1 (MQ=255)        tGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGc                                                                                                                          <  2:112107/140‑1 (MQ=255)                 cAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTAc                                                                                                                 <  1:565704/140‑1 (MQ=255)                  aaGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTAcc                                                                                                                <  1:1114143/140‑1 (MQ=255)                    gCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCgg                                                                                                              <  2:632021/140‑1 (MQ=255)                              cGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATa                                                                                                    >  1:343241/1‑140 (MQ=255)                              cGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATa                                                                                                    >  1:507523/1‑140 (MQ=255)                                     ccGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATc                                                                                             <  2:507523/140‑1 (MQ=255)                                     ccGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATc                                                                                             <  2:143629/140‑1 (MQ=255)                                              tGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGt                                                                                    >  2:239322/1‑140 (MQ=255)                                               gCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTa                                                                                   >  2:769820/1‑140 (MQ=255)                                                           gcgGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCa                                                                       <  1:754561/140‑1 (MQ=255)                                                            cgGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAg                                                                      <  2:96364/140‑1 (MQ=255)                                                            cgGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAg                                                                      <  2:700888/140‑1 (MQ=255)                                                             ggCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGc                                                                     <  1:769820/140‑1 (MQ=255)                                                                      aCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGc                                                            <  1:224728/140‑1 (MQ=255)                                                                                                tCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGcc                                  >  2:477498/1‑140 (MQ=255)                                                                                                  gCACAGCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGa                                >  2:941146/1‑140 (MQ=255)                                                                                                       gCGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAg                           >  1:823172/1‑140 (MQ=255)                                                                                                        cGGACGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGc                          >  2:66823/1‑140 (MQ=255)                                                                                                           aCGGCGCATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGa                       <  2:955249/140‑1 (MQ=255)                                                                                                              gcgcATTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGAttt                    >  1:672931/1‑140 (MQ=255)                                                                                                                  aTTGTGCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCa                >  2:402421/1‑140 (MQ=255)                                                                                                                    tgtgCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAgg              >  1:402415/1‑140 (MQ=255)                                                                                                                     gtgCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGc             <  1:66823/140‑1 (MQ=255)                                                                                                                       gCTGGCGACCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGt           <  2:672931/140‑1 (MQ=255)                                                                                                                              aCCAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCaa    >  1:1024240/1‑140 (MQ=255)                                                                                                                                cAACGCCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATc  >  1:642794/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       CCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATC  >  NC_000913/1483977‑1484243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑