Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,731,481	A→G	T799A (ACC→GCC)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,731,481	0	A	G	100.0%	127.4 / NA	36	T799A (ACC→GCC)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

TGAACCAGAAAAGTTGCTGCAAATTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTT  >  NC_000913/1731357‑1731616                                                                                                                             |                                                                                                                                        tgAACCAGAAAAGTTGCTGCAAATTGTCCGCGAGGCGGTAGGCAGTTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGcaac                                                                                                                          >  2:284352/1‑140 (MQ=255) tgAACCAGAAAAGTTGCTGCAAATTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGcaac                                                                                                                          >  2:809499/1‑140 (MQ=255)      cAGAAAAGTTGCTGCAAATTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACgg                                                                                                                     <  2:748466/140‑1 (MQ=255)              ttGCTGCAAATTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTgcgcgc                                                                                                             <  1:124104/140‑1 (MQ=255)                       aTTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCcagtcag                                                                                                      <  1:170388/138‑1 (MQ=255)                       aTTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCcagtcag                                                                                                      >  2:170388/1‑138 (MQ=255)                                    cGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCt                                                                                       <  2:91330/140‑1 (MQ=255)                                    cGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCt                                                                                       <  2:699540/140‑1 (MQ=255)                                        aGGCAGTTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAgg                                                                                   <  1:284352/140‑1 (MQ=255)                                              cTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATc                                                                             >  1:633816/1‑140 (MQ=255)                                              cTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATc                                                                             >  1:714653/1‑140 (MQ=255)                                              cTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCCGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATc                                                                             >  1:1148496/1‑140 (MQ=255)                                                             cgccCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCa                                                              <  1:809499/140‑1 (MQ=255)                                                             cgccCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCa                                                              <  2:714653/140‑1 (MQ=255)                                                             cgccCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCCCTCAGGGATATCCGGATTTTCCGGTCa                                                              <  2:408580/140‑1 (MQ=255)                                                               cccGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCAtt                                                            >  1:11682/1‑140 (MQ=255)                                                                ccGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATtc                                                           >  1:400577/1‑140 (MQ=255)                                                                        gcgAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAAcc                                                   <  2:400577/140‑1 (MQ=255)                                                                        gcgAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAAcc                                                   <  2:557218/140‑1 (MQ=255)                                                                             tGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGa                                                       <  2:409602/131‑1 (MQ=255)                                                                             tGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGa                                                       >  1:409602/1‑131 (MQ=255)                                                                                gccgcGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGa                                           <  2:11682/140‑1 (MQ=255)                                                                                           attattAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                                <  2:633816/140‑1 (MQ=255)                                                                                           attattAATGCCGGGGCGCACTACGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                                <  1:519650/140‑1 (MQ=255)                                                                                                   tGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                                <  1:867385/132‑1 (MQ=255)                                                                                                   tGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                                >  2:867385/1‑132 (MQ=255)                                                                                                   tGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTAt                        >  2:957992/1‑140 (MQ=255)                                                                                                   tGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTAt                        >  1:1058210/1‑140 (MQ=255)                                                                                                     ccGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGa                      <  1:957992/140‑1 (MQ=255)                                                                                                       ggggCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                                >  2:824975/1‑128 (MQ=255)                                                                                                       ggggCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                                <  1:824975/128‑1 (MQ=255)                                                                                                        gggCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCaa                                 >  2:788507/1‑126 (MQ=255)                                                                                                        gggCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCaa                                 <  1:788507/126‑1 (MQ=255)                                                                                                         ggCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCtt                  >  2:859750/1‑140 (MQ=255)                                                                                                          gcgcACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTc                 <  2:935584/140‑1 (MQ=255)                                                                                                                         tCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGtt  >  2:335571/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                        TGAACCAGAAAAGTTGCTGCAAATTGTCCGCGAGGCGGTAGGCAGCTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTT  >  NC_000913/1731357‑1731616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑