Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	110,714	A→G	A812A (GCA→GCG)	secA →	protein translocation ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	110,714	0	A	G	100.0%	263.0 / NA	72	A812A (GCA→GCG)	secA	protein translocation ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/40);  total (32/40)

TGCTGCAAACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTA  >  NC_000913/110578‑110850                                                                                                                                         |                                                                                                                                         tgctgcAAACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTgcggcg                                                                                                                                       >  1:130391/1‑140 (MQ=255)    tgcAAACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATg                                                                                                                                    <  2:130391/140‑1 (MQ=255)          cGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAg                                                                                                                              <  2:230483/140‑1 (MQ=255)           gCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGt                                                                                                                             <  1:531982/140‑1 (MQ=255)           gCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGt                                                                                                                             >  1:387264/1‑140 (MQ=255)           gCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGt                                                                                                                             <  2:8586/140‑1 (MQ=255)             ttGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCg                                                                                                                           >  1:458358/1‑140 (MQ=255)              tGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCTGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTgcggcg                                                                                                                                       <  2:967627/127‑1 (MQ=255)              tGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCTGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTgcggcg                                                                                                                                       >  1:967627/1‑127 (MQ=255)               gACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGtt                                                                                                                         <  1:33524/140‑1 (MQ=255)                     cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGt                                                                                                                             <  2:678069/130‑1 (MQ=255)                     cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGt                                                                                                                             >  1:678069/1‑130 (MQ=255)                     cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTg                                                                                                                        <  1:250931/135‑1 (MQ=255)                     cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTg                                                                                                                        >  2:250931/1‑135 (MQ=255)                     cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAata                                                                                                                   >  2:655915/1‑140 (MQ=255)                     cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAata                                                                                                                   >  2:857685/1‑140 (MQ=255)                       gtgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATg                                                                                                                 <  2:527157/140‑1 (MQ=255)                       gtgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATg                                                                                                                 <  1:569452/140‑1 (MQ=255)                        tgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGa                                                                                                                >  2:582070/1‑140 (MQ=255)                        tgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGa                                                                                                                <  1:1126573/140‑1 (MQ=255)                         ggAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGTAATATGaa                                                                                                               >  2:1064692/1‑140 (MQ=255)                           aaaGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGt                                                                                                             <  1:230442/140‑1 (MQ=255)                           aaaGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGt                                                                                                             <  1:417746/140‑1 (MQ=255)                             agagCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTa                                                                                                           <  2:346995/140‑1 (MQ=255)                              gagCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTAt                                                                                                          >  2:197943/1‑140 (MQ=255)                               agCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATc                                                                                                         >  2:453302/1‑140 (MQ=255)                                 cACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAg                                                                                                       >  2:955974/1‑140 (MQ=255)                                  aCCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGt                                                                                                      <  2:462284/140‑1 (MQ=255)                                  aCCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGt                                                                                                      <  2:344056/140‑1 (MQ=255)                                     tGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACg                                                                                                   >  1:24927/1‑140 (MQ=255)                                      ggCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGc                                                                                                  <  2:458358/140‑1 (MQ=255)                                          gCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAg                                                                                              <  2:1114375/140‑1 (MQ=255)                                                    aTCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAg                                                                                    <  2:965601/140‑1 (MQ=255)                                                    aTCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAg                                                                                    <  1:582070/140‑1 (MQ=255)                                                    aTCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAAACGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAg                                                                                    <  1:822299/140‑1 (MQ=255)                                                        gCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTAc                                                                                >  1:231847/1‑140 (MQ=255)                                                          gTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGt                                                                              >  1:1079964/1‑140 (MQ=255)                                                           tCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTa                                                                             >  2:1069570/1‑140 (MQ=255)                                                            cAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTAt                                                                            <  2:387264/140‑1 (MQ=255)                                                               ggTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGcc                                                                         >  2:1127852/1‑140 (MQ=255)                                                                  aTCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATGAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGa                                                                      <  1:197943/140‑1 (MQ=255)                                                                  aTCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGa                                                                      <  2:1079964/140‑1 (MQ=255)                                                                   tCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGaa                                                                     >  1:881588/1‑140 (MQ=255)                                                                    ccACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAag                                                                    <  2:1044324/140‑1 (MQ=255)                                                                      aCCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAagag                                                                  <  1:195711/140‑1 (MQ=255)                                                                        cTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGt                                                                >  2:487730/1‑140 (MQ=255)                                                                           cGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTga                                                             >  1:851182/1‑140 (MQ=255)                                                                           cGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTga                                                             >  1:1080949/1‑140 (MQ=255)                                                                             tGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCCTGTTTGCGGCGATGCTGGCGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCCAAGATCAGGCACGTATGCCTGAAGAGGTTgagg                                                           >  1:390176/1‑140 (MQ=255)                                                                             tGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgagg                                                           >  2:755690/1‑140 (MQ=255)                                                                             tGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgagg                                                           >  1:537308/1‑140 (MQ=255)                                                                              ggCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgagga                                                          <  1:453302/140‑1 (MQ=255)                                                                               gCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgaggag                                                         >  1:872069/1‑140 (MQ=255)                                                                                cTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGc                                                        <  2:851182/140‑1 (MQ=255)                                                                                cTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGc                                                        <  2:803535/140‑1 (MQ=255)                                                                                cTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGc                                                        <  1:996888/140‑1 (MQ=255)                                                                                cTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGc                                                        >  1:445391/1‑140 (MQ=255)                                                                                  aCGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGACGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTg                                                      >  2:115266/1‑140 (MQ=255)                                                                                   cGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTgg                                                     <  2:402692/140‑1 (MQ=255)                                                                                        aGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGaacaa                                                >  1:435634/1‑140 (MQ=255)                                                                                          aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGTAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAacaaca                                              <  1:1064692/140‑1 (MQ=255)                                                                                          aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAacaaca                                              <  1:975573/140‑1 (MQ=255)                                                                                           aaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAg                                             <  1:1127852/140‑1 (MQ=255)                                                                                           aaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAg                                             <  1:520742/140‑1 (MQ=255)                                                                                              aTCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgt                                          >  1:514692/1‑140 (MQ=255)                                                                                              aTCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGACGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgt                                          <  1:115266/140‑1 (MQ=255)                                                                                               tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAacaaca                                              >  2:676980/1‑135 (MQ=255)                                                                                               tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAacaaca                                              <  1:676980/135‑1 (MQ=255)                                                                                                    aGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATg                                    >  2:437840/1‑140 (MQ=255)                                                                                                            tACAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGa                            <  2:514692/140‑1 (MQ=255)                                                                                                             aCAAACGTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAg                           <  2:463860/140‑1 (MQ=255)                                                                                                                   gTGAATCGTTCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTa                     >  1:313310/1‑140 (MQ=255)                                                                                                                           ttCTCCATGTTTGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAAt             >  2:455185/1‑140 (MQ=255)                                                                                                                                    tttGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCt    <  1:1069570/140‑1 (MQ=255)                                                                                                                                      tGCGGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTa  <  2:24927/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGCTGCAAACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTA  >  NC_000913/110578‑110850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑