Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,538	A→G	S148P (TCC→CCC)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,538	0	A	G	100.0%	241.9 / NA	66	S148P (TCC→CCC)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/35);  total (31/35)

TGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGC  >  NC_000913/1853401‑1853670                                                                                                                                          |                                                                                                                                     tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGcc                                                                                                                                    >  1:895867/1‑140 (MQ=255)  gagagaAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCACGCGGGCCa                                                                                                                                   >  1:183211/1‑140 (MQ=255)   agagaAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCAt                                                                                                                                  >  1:809178/1‑140 (MQ=255)   agagaAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCAt                                                                                                                                  >  2:53279/1‑140 (MQ=255)    gagaAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCAtt                                                                                                                                 <  1:737330/140‑1 (MQ=255)             ggCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCa                                                                                                                        >  2:667262/1‑140 (MQ=255)              gCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCAc                                                                                                                       >  2:900233/1‑140 (MQ=255)                   cAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTcg                                                                                                                  <  1:958632/140‑1 (MQ=255)                   cAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTcg                                                                                                                  <  2:155287/140‑1 (MQ=255)                   cAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTcg                                                                                                                  <  1:33674/140‑1 (MQ=255)                     gTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTcgcg                                                                                                                <  2:895867/140‑1 (MQ=255)                        tGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACGAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGTggag                                                                                                             >  2:1070356/1‑138 (MQ=255)                        tGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTcgcg                                                                                                                <  2:654859/137‑1 (MQ=255)                        tGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTcgcg                                                                                                                >  1:654859/1‑137 (MQ=255)                        tGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCgggg                                                                                                             >  2:678990/1‑140 (MQ=255)                        tGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCgggg                                                                                                             >  2:324975/1‑140 (MQ=255)                        tGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCgggg                                                                                                             >  1:193057/1‑140 (MQ=255)                                 caccCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTcc                                                                                                    <  1:117115/140‑1 (MQ=255)                                  accCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATACCCCCCAGGCCTACCGCGGCCGACAGAAAGGCGTCCCAGTTACCACCAAATGAGAGCCGCGCGGGCCATTTTCCACCCACCGACGCGGGGAAAAACTcct                                                                                                   >  2:235835/1‑139 (MQ=255)                                  accCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCg                                                                                                   >  2:220507/1‑140 (MQ=255)                                  accCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCg                                                                                                   >  2:168208/1‑140 (MQ=255)                                   cccAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGt                                                                                                  >  1:292566/1‑140 (MQ=255)                                   cccAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGt                                                                                                  >  2:415663/1‑140 (MQ=255)                                   cccAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGt                                                                                                  >  2:432038/1‑140 (MQ=255)                                    ccAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTa                                                                                                 >  1:940443/1‑140 (MQ=255)                                       gcagAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAAt                                                                                              <  1:415663/140‑1 (MQ=255)                                       gcagAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAAt                                                                                              <  1:432038/140‑1 (MQ=255)                                           aaaCATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAgg                                                                                          <  1:900233/140‑1 (MQ=255)                                             aCATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCa                                                                                        <  2:940443/140‑1 (MQ=255)                                             aCATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCa                                                                                        <  2:399193/140‑1 (MQ=255)                                             aCATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCa                                                                                        <  2:609126/140‑1 (MQ=255)                                             aCATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCa                                                                                        <  1:192560/140‑1 (MQ=255)                                               attattCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCCACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATa                                                                                      <  1:1070356/140‑1 (MQ=255)                                                ttattCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAg                                                                                     <  1:667262/140‑1 (MQ=255)                                                 tattTGCCCACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    <  1:235835/140‑1 (MQ=255)                                                 tattCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    <  1:220507/140‑1 (MQ=255)                                                 tattCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    <  1:974494/140‑1 (MQ=255)                                                 tattCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    >  2:384993/1‑140 (MQ=255)                                                 tattCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCACGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    <  2:183211/140‑1 (MQ=255)                                                  attCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGGCAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    >  2:37302/1‑139 (MQ=255)                                                  attCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGGCAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGc                                                                                    <  1:37302/139‑1 (MQ=255)                                                  attCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTcc                                                                                                    >  1:853417/1‑123 (MQ=255)                                                  attCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTcc                                                                                                    <  2:853417/123‑1 (MQ=255)                                                         aaCTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATa                                                                            <  2:292566/140‑1 (MQ=255)                                                               aaaaaGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAgc                                                                      <  1:1095569/140‑1 (MQ=255)                                                                 aaaGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAgcgc                                                                    <  1:53279/140‑1 (MQ=255)                                                                     cGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGccccc                                                                >  2:761909/1‑140 (MQ=255)                                                                      gATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGcccc                                                                 >  1:570756/1‑138 (MQ=255)                                                                      gATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGcccc                                                                 <  2:570756/138‑1 (MQ=255)                                                                      gATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCa                                                               >  1:715224/1‑140 (MQ=255)                                                                      gATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCa                                                               >  2:466804/1‑140 (MQ=255)                                                                         aaCCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTc                                                            <  1:466804/140‑1 (MQ=255)                                                                                                    cATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                 <  1:306005/140‑1 (MQ=255)                                                                                                    cATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                 <  1:168208/140‑1 (MQ=255)                                                                                                    cATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                 <  1:678990/140‑1 (MQ=255)                                                                                                        ggCGACCAGTTACCAACAAATGATAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAg                             >  1:1002513/1‑140 (MQ=255)                                                                                                         gCGACCAGTTACCAACAAATGATAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGc                            <  2:1002513/140‑1 (MQ=255)                                                                                                         gCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGc                            <  2:715224/140‑1 (MQ=255)                                                                                                         gCGACCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGc                            <  1:761909/140‑1 (MQ=255)                                                                                                            aCCAGTTACCAACAAATGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAg                         >  1:1028247/1‑140 (MQ=255)                                                                                                                           aTGAGAGCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACa          >  1:166366/1‑140 (MQ=255)                                                                                                                              agagCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACaaaa       >  1:902635/1‑140 (MQ=255)                                                                                                                                agCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAg      <  2:338384/139‑1 (MQ=255)                                                                                                                                agCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAg      >  1:338384/1‑139 (MQ=255)                                                                                                                                agCCGCGCGGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGc     >  1:200012/1‑140 (MQ=255)                                                                                                                                     cgcgGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGc  <  1:191523/138‑1 (MQ=255)                                                                                                                                     cgcgGGCCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGc  >  2:191523/1‑138 (MQ=255)                                                                                                                                          |                                                                                                                                     TGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGC  >  NC_000913/1853401‑1853670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑