Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,778	T→C	P185P (CCA→CCG)	ydjL ←	putative zinc‑binding dehydrogenase YdjL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,778	0	T	C	100.0%	217.8 / NA	60	P185P (CCA→CCG)	ydjL	putative zinc‑binding dehydrogenase YdjL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/33);  total (27/33)

GTAGAACCATTTACTACTGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCA  >  NC_000913/1860641‑1860909                                                                                                                                          |                                                                                                                                    gTAGAACCATTTACTACTGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCgg                                                                                                                                   >  2:680357/1‑140 (MQ=255)  tAGAACCATTTACTACTGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCggg                                                                                                                                  <  1:385223/140‑1 (MQ=255)   aGAACCATTTACTACTGCCGCCGCCCCCATTTCTTTTGCACCGGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGc                                                                                                                                 <  1:673307/140‑1 (MQ=255)     aaCCATTTACTACTGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCa                                                                                                                               >  1:90373/1‑140 (MQ=255)          tttACTACTGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGcc                                                                                                                          >  1:860914/1‑140 (MQ=255)                 ctGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATgacg                                                                                                                   >  1:983168/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  1:805846/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  1:127159/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  1:199397/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  1:279094/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  2:695989/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  2:564611/1‑140 (MQ=255)                    ccGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGAcc                                                                                                                >  1:347549/1‑140 (MQ=255)                              cttttCTTTCGCAACCGGGAGGGGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGAc                                                                                                      <  2:805846/137‑1 (MQ=255)                              cggTTCTTTGCCAACGGGGAGGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGAc                                                                                                      <  2:347549/138‑1 (MQ=255)                                 ttCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCTTAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCag                                                                                                   >  1:932356/1‑140 (MQ=255)                                 ttCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCag                                                                                                   >  2:159997/1‑140 (MQ=255)                                    ttttGCACCGGGGAGGGGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCaggaa                                                                                                <  2:279094/140‑1 (MQ=255)                                           aCCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATc                                                                                                           <  1:176827/122‑1 (MQ=255)                                           aCCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATc                                                                                                           >  2:176827/1‑122 (MQ=255)                                               ggAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGAt                                                                                     <  2:849045/140‑1 (MQ=255)                                                   gCGGGCCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATtgct                                                                                 <  1:695989/140‑1 (MQ=255)                                                           ccACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATg                                                                         <  2:493634/140‑1 (MQ=255)                                                                  ttcttGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGt                                                                  <  1:832871/140‑1 (MQ=255)                                                                  ttcttGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGt                                                                  <  1:564611/140‑1 (MQ=255)                                                                    cttGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAg                                                                <  1:659505/140‑1 (MQ=255)                                                                     ttGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAgg                                                               <  2:983168/140‑1 (MQ=255)                                                                     ttGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAg                                                                >  2:4500/1‑139 (MQ=255)                                                                     ttGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAg                                                                <  1:4500/139‑1 (MQ=255)                                                                      tGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTgcgc                                                                             <  2:1143496/125‑1 (MQ=255)                                                                      tGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTgcgc                                                                             >  1:1143496/1‑125 (MQ=255)                                                                         aGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGa                                                                           <  1:733751/124‑1 (MQ=255)                                                                         aGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGa                                                                           >  2:733751/1‑124 (MQ=255)                                                                         aGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCAtt                                                           <  1:1115120/140‑1 (MQ=255)                                                                         aGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCAtt                                                           <  1:159997/140‑1 (MQ=255)                                                                           aCCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAgg                                                               >  2:849268/1‑134 (MQ=255)                                                                           aCCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAgg                                                               <  1:849268/134‑1 (MQ=255)                                                                           aCCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTAc                                                         >  1:296253/1‑140 (MQ=255)                                                                              aaCGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGa                                                      <  1:602201/140‑1 (MQ=255)                                                                               acgacgacgATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGAt                                                     <  1:680357/140‑1 (MQ=255)                                                                                cgacgacgaTATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATa                                                    <  2:864670/140‑1 (MQ=255)                                                                                  acgacgaTATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAgg                                                  <  2:127159/140‑1 (MQ=255)                                                                                   cgacgaTATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAggg                                                 <  1:970757/140‑1 (MQ=255)                                                                                                cGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAggg                                                 >  1:66/1‑127 (MQ=255)                                                                                                cGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAggg                                                 <  2:66/127‑1 (MQ=255)                                                                                                   ccccATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGt                                 >  1:1109162/1‑140 (MQ=255)                                                                                                   ccccATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGt                                 >  2:286085/1‑140 (MQ=255)                                                                                                       aTAATTCGCGCCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTc                             >  2:1143974/1‑140 (MQ=255)                                                                                                               cgcCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCaa                     <  1:634088/140‑1 (MQ=255)                                                                                                               cgcCATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCaa                     >  1:1121942/1‑140 (MQ=255)                                                                                                                 ccATTTGTACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAaca                   <  2:296253/140‑1 (MQ=255)                                                                                                                        tACGGAGAACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAg            <  1:286085/140‑1 (MQ=255)                                                                                                                            gagaACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGa         <  1:425450/139‑1 (MQ=255)                                                                                                                            gagaACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGa         >  2:425450/1‑139 (MQ=255)                                                                                                                              gaACAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGAttt      >  1:894780/1‑140 (MQ=255)                                                                                                                               aaCAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGa         <  2:662107/136‑1 (MQ=255)                                                                                                                               aaCAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGa         >  1:11490/1‑136 (MQ=255)                                                                                                                               aaCAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGa         >  1:662107/1‑136 (MQ=255)                                                                                                                               aaCAGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGa         <  2:11490/136‑1 (MQ=255)                                                                                                                                  aGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCca  <  2:932356/140‑1 (MQ=255)                                                                                                                                  aGCCCGAGCGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCca  <  2:1109162/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GTAGAACCATTTACTACTGCCGTCGCACCCAGTTCTTTTGCAACCGGGAAGCGGACCGCCACATCTTCTTGCAGACCAACGACGACGATATTTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCA  >  NC_000913/1860641‑1860909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑