Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,951,064	T→C	V78A (GTG→GCG)	yecD →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,951,064	0	T	C	100.0%	206.4 / NA	57	V78A (GTG→GCG)	yecD	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/35);  total (22/35)

AGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACA  >  NC_000913/1950930‑1951200                                                                                                                                       |                                                                                                                                         aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGc                                                                                                                                     >  2:1046714/1‑140 (MQ=255) aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGc                                                                                                                                     >  2:892483/1‑140 (MQ=255)      aTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGaa                                                                                                                                <  2:720399/140‑1 (MQ=255)          cgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAAtt                                                                                                                            >  2:145876/1‑140 (MQ=255)            ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATtgg                                                                                                                          <  1:974795/140‑1 (MQ=255)            ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATtgg                                                                                                                          <  1:388309/140‑1 (MQ=255)            ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATtgg                                                                                                                          >  2:314724/1‑140 (MQ=255)             cGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATtggt                                                                                                                         >  1:973077/1‑140 (MQ=255)                    cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACa                                                                                                                  <  1:145876/140‑1 (MQ=255)                    cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACa                                                                                                                  <  1:314724/140‑1 (MQ=255)                    cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACa                                                                                                                  <  1:1095845/140‑1 (MQ=255)                    cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACa                                                                                                                  >  1:739834/1‑140 (MQ=255)                    cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACa                                                                                                                  <  1:1046714/140‑1 (MQ=255)                     tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATGACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACAt                                                                                                                 >  2:1017708/1‑140 (MQ=255)                      ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATc                                                                                                                <  2:860852/140‑1 (MQ=255)                      ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATc                                                                                                                <  1:1017708/140‑1 (MQ=255)                      ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATc                                                                                                                <  2:124773/140‑1 (MQ=255)                        cggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCct                                                                                                              <  1:74481/140‑1 (MQ=255)                         ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCctg                                                                                                             <  2:507332/140‑1 (MQ=255)                           cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCctgct                                                                                                           >  1:432431/1‑140 (MQ=255)                                       ccAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCa                                                                                               <  1:1152301/140‑1 (MQ=255)                                       ccAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCa                                                                                               <  1:981759/140‑1 (MQ=255)                                           cGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCg                                                                                           <  2:973077/140‑1 (MQ=255)                                                 gCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCACTAGGTGCAACCGACAGCg                                                                                     <  2:124737/140‑1 (MQ=255)                                                    cGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGata                                                                                  >  2:220859/1‑140 (MQ=255)                                                      tgtTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATc                                                                                >  1:219440/1‑140 (MQ=255)                                                                gcgcGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAAtcatca                                                                      >  1:313489/1‑140 (MQ=255)                                                                gcgcGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAAtcatca                                                                      >  2:317712/1‑140 (MQ=255)                                                                  gcgTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCaaa                                                                    <  2:810434/140‑1 (MQ=255)                                                                  gcgTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCaaa                                                                    <  2:313489/140‑1 (MQ=255)                                                                   cgTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAAc                                                                   >  2:198837/1‑140 (MQ=255)                                                                             tCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATgggg                                                         >  1:668073/1‑140 (MQ=255)                                                                                    aTTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGttt                                                  <  1:317712/140‑1 (MQ=255)                                                                                      tACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGTAAGGATGAAACGTCAATGGGGTGCGTTTta                                                >  2:810004/1‑140 (MQ=255)                                                                                      tACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTta                                                <  1:431618/140‑1 (MQ=255)                                                                                       aCGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTtac                                               >  1:169687/1‑140 (MQ=255)                                                                                       aCGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTtac                                               >  1:914113/1‑140 (MQ=255)                                                                                           tGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTtacggta                                           <  2:1086517/140‑1 (MQ=255)                                                                                                  ttAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCt                                    >  2:74619/1‑140 (MQ=255)                                                                                                         aGCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTg                             >  1:228691/1‑140 (MQ=255)                                                                                                          gCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGc                            >  2:960635/1‑140 (MQ=255)                                                                                                          gCCGGTCGATGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGc                            >  2:910119/1‑140 (MQ=255)                                                                                                                  aTGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTAcgc                    <  1:892483/140‑1 (MQ=255)                                                                                                                   tGCGCCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTAcgcc                   >  2:392068/1‑140 (MQ=255)                                                                                                                    gcgcCCTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTAcgccg                  <  2:911711/140‑1 (MQ=255)                                                                                                                        ccTCACCGGCTAAAGCGTTGCCCGAATATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGa              <  1:810004/140‑1 (MQ=255)                                                                                                                         cTCACCGGCTAACGCGTTGCCCGAGAATTGGTGGCAACACCCTGCGCCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGCGGGCGTTCTACGGTACGGATATGGGGCTGCAATTAGGGCGCCGAg             >  2:55087/1‑140 (MQ=255)                                                                                                                         cTCACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAg             <  2:668073/140‑1 (MQ=255)                                                                                                                           cACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGt           <  1:220859/140‑1 (MQ=255)                                                                                                                           cACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGt           <  2:169687/140‑1 (MQ=255)                                                                                                                           cACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGt           <  1:198837/140‑1 (MQ=255)                                                                                                                           cACCGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGt           <  2:914113/140‑1 (MQ=255)                                                                                                                             ccGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTAt         <  2:783542/140‑1 (MQ=255)                                                                                                                             ccGGCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTAt         <  1:308784/140‑1 (MQ=255)                                                                                                                               ggCTAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCg       <  2:191081/140‑1 (MQ=255)                                                                                                                                  tAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATa    <  2:612794/140‑1 (MQ=255)                                                                                                                                  tAAAGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATa    <  2:219440/140‑1 (MQ=255)                                                                                                                                    aaGCGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACa  <  1:74619/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACA  >  NC_000913/1950930‑1951200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑