Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,037,372	T→C	T261A (ACG→GCG)	hprS ←	sensory histidine kinase HprS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,037,372	0	T	C	100.0%	219.2 / NA	60	T261A (ACG→GCG)	hprS	sensory histidine kinase HprS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

TTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCAAGAG  >  NC_000913/2037236‑2037503                                                                                                                                         |                                                                                                                                    ttCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTG‑TTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAAcc                                                                                                                                 <  2:616565/140‑1 (MQ=255)     ccAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGa                                                                                                                              >  2:1070804/1‑140 (MQ=255)     ccAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGa                                                                                                                              >  2:665104/1‑140 (MQ=255)       aaaaCATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGAtt                                                                                                                            <  2:986103/140‑1 (MQ=255)        aaaCATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTc                                                                                                                           <  2:762823/140‑1 (MQ=255)             ttGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGAcc                                                                                                                      <  1:869163/140‑1 (MQ=255)             ttGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGAcc                                                                                                                      <  1:668835/140‑1 (MQ=255)                       cTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCa                                                                                                            <  1:1070804/140‑1 (MQ=255)                               gCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAAt                                                                                                               >  1:1151138/1‑129 (MQ=255)                               gCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAAt                                                                                                               <  2:1151138/129‑1 (MQ=255)                                   gAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGtt                                                                                                <  2:722907/140‑1 (MQ=255)                                   gAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGtt                                                                                                <  2:301962/140‑1 (MQ=255)                                   gAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGtt                                                                                                <  1:203564/140‑1 (MQ=255)                                    aaaCAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTc                                                                                               >  1:859490/1‑140 (MQ=255)                                         gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTg                                                                                                     >  2:1117702/1‑129 (MQ=255)                                         gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTg                                                                                                     <  1:1117702/129‑1 (MQ=255)                                         gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGt                                                                                          >  2:335113/1‑140 (MQ=255)                                         gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGt                                                                                          >  2:153928/1‑140 (MQ=255)                                           aTGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTc                                                                                        <  2:859490/140‑1 (MQ=255)                                             gTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCAt                                                                                      >  1:630064/1‑140 (MQ=255)                                               tctcTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGa                                                                                    >  2:298713/1‑140 (MQ=255)                                                   tGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCga                                                                                >  1:1080531/1‑140 (MQ=255)                                                   tGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCga                                                                                >  1:953703/1‑140 (MQ=255)                                                    gTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCt                                                                                              <  2:324479/125‑1 (MQ=255)                                                    gTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCt                                                                                              >  1:324479/1‑125 (MQ=255)                                                    gTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCgag                                                                               >  2:352220/1‑140 (MQ=255)                                                    gTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCgag                                                                               >  1:828140/1‑140 (MQ=255)                                                      tAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGAt                                                                             <  1:695926/140‑1 (MQ=255)                                                        aCCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCg                                                                           >  2:995302/1‑140 (MQ=255)                                                        aCCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCg                                                                           >  1:776216/1‑140 (MQ=255)                                                        aCCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCg                                                                           >  1:619584/1‑140 (MQ=255)                                                        aCCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCg                                                                           >  1:284847/1‑140 (MQ=255)                                                              aaaTATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCa                                                                     <  1:298713/140‑1 (MQ=255)                                                                           ctctTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGAc                                                        >  1:422972/1‑140 (MQ=255)                                                                           ctctTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGAc                                                        >  1:318329/1‑140 (MQ=255)                                                                            tctTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACg                                                       >  2:398689/1‑140 (MQ=255)                                                                              ttCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCt                                                     >  2:836010/1‑140 (MQ=255)                                                                                cAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCa                                                   >  2:347136/1‑140 (MQ=255)                                                                                        ccGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAg                                                          >  1:1083053/1‑125 (MQ=255)                                                                                        ccGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAg                                                          <  2:1083053/125‑1 (MQ=255)                                                                                        ccGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCttt                                           >  1:722488/1‑140 (MQ=255)                                                                                            cAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACt                                       <  2:87225/140‑1 (MQ=255)                                                                                            cAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACt                                       <  1:153928/140‑1 (MQ=255)                                                                                                    ttttGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCatg                               <  2:318329/140‑1 (MQ=255)                                                                                                    ttttGATATTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCatg                               <  2:422972/140‑1 (MQ=255)                                                                                                         atatTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCatgat                             >  2:434754/1‑137 (MQ=255)                                                                                                         atatTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCatgat                             <  1:434754/137‑1 (MQ=255)                                                                                                           atTCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCAtt                        <  1:495523/140‑1 (MQ=255)                                                                                                            ttCAGCGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCAttt                       <  2:284847/140‑1 (MQ=255)                                                                                                                 cGATACTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCACTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTc                  <  2:1080531/140‑1 (MQ=255)                                                                                                                      cTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGc             >  2:68470/1‑140 (MQ=255)                                                                                                                      cTTCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGc             >  1:267693/1‑140 (MQ=255)                                                                                                                       ttCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGcc            >  1:332902/1‑140 (MQ=255)                                                                                                                       ttCTGGTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGcc            <  1:997138/140‑1 (MQ=255)                                                                                                                            gTTTGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGccc       >  2:77640/1‑140 (MQ=255)                                                                                                                              ttGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCaa     <  1:398689/140‑1 (MQ=255)                                                                                                                               tGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCAag    >  1:1057314/1‑140 (MQ=255)                                                                                                                               tGACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCAag    >  2:932297/1‑140 (MQ=255)                                                                                                                                gACTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCAaga   <  2:1057314/140‑1 (MQ=255)                                                                                                                                 aCTGAGCGCAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCAagag  >  1:137659/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAGACGCTCAAAATCTTTGACTAAAGCATGATGCATTTTATTCAACGCCTGCCCAAGAG  >  NC_000913/2037236‑2037503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑