Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	123,000	T→C	intergenic (+144/‑17)	pdhR → / → aceE	DNA‑binding transcriptional dual regulator PdhR/pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	123,000	0	T	C	100.0%	207.9 / NA	57	intergenic (+144/‑17)	pdhR/aceE	DNA‑binding transcriptional dual regulator PdhR/pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCA  >  NC_000913/122865‑123132                                                                                                                                        |                                                                                                                                     cTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTagacagat                                                                                                                                  <  1:787120/140‑1 (MQ=255)                        gttgtAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACg                                                                                                           >  1:24907/1‑140 (MQ=255)                          tgtAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGtt                                                                                                         >  1:762289/1‑140 (MQ=255)                           gtAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGAAATAACCCATGTCAGAACGttt                                                                                                        >  2:1115448/1‑140 (MQ=255)                                 cAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGAAATAACCCATGTCAGAACGTTTCCCaaa                                                                                                  <  1:1115448/140‑1 (MQ=255)                                   aGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATg                                                                                                >  1:318773/1‑140 (MQ=255)                                         tataAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTgg                                                                                          >  2:371876/1‑140 (MQ=255)                                          ataAAAGCGCGGCAACTAAACGTAGAACC‑GTCTTATTGAGCTTTCCGGCGAGTGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGAt                                                                                        <  1:1107019/140‑1 (MQ=255)                                          ataAAAGCGCGGCAACTAAACGTAGAACC‑GTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGAt                                                                                        <  1:370108/140‑1 (MQ=255)                                            aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATc                                                                                       <  1:499540/140‑1 (MQ=255)                                               aGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGa                                                                                    >  2:847875/1‑140 (MQ=255)                                                  gcgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTGTTACACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCg                                                                                 >  2:71193/1‑140 (MQ=255)                                                  gcgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCg                                                                                 >  1:537170/1‑140 (MQ=255)                                                   cgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGa                                                                                >  1:485630/1‑140 (MQ=255)                                                   cgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGa                                                                                >  1:157960/1‑140 (MQ=255)                                                    ggCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGaa                                                                               >  2:58048/1‑140 (MQ=255)                                                            aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTGTTACACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGa                                                                       <  1:71193/140‑1 (MQ=255)                                                            aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGa                                                                       <  2:762289/140‑1 (MQ=255)                                                             aCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGAc                                                                      <  2:803224/140‑1 (MQ=255)                                                                        gTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGa                                                                       <  2:842936/128‑1 (MQ=255)                                                                        gTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGa                                                                       >  1:842936/1‑128 (MQ=255)                                                                        gTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGc                                                           >  1:860165/1‑140 (MQ=255)                                                                        gTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGc                                                           >  2:470185/1‑140 (MQ=255)                                                                              ttGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGa                                                     >  1:743260/1‑140 (MQ=255)                                                                              ttGAGCTTTCCGGCGAGAGTGCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGa                                                     >  2:859101/1‑140 (MQ=255)                                                                               tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCa                                                              <  2:569604/130‑1 (MQ=255)                                                                               tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCa                                                              >  1:569604/1‑130 (MQ=255)                                                                                   cTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCgg                                                >  1:692464/1‑140 (MQ=255)                                                                                    tttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                               <  2:692464/140‑1 (MQ=255)                                                                                    tttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                               <  1:111275/140‑1 (MQ=255)                                                                                    tttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                               <  1:859101/140‑1 (MQ=255)                                                                                      tCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCa                                             <  1:275207/140‑1 (MQ=255)                                                                                                 ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaagaa                                  <  2:537170/140‑1 (MQ=255)                                                                                                         gACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaaga                                   >  2:589350/1‑131 (MQ=255)                                                                                                         gACAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaaga                                   <  1:589350/131‑1 (MQ=255)                                                                                                           cAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaag                                    <  1:593392/128‑1 (MQ=255)                                                                                                           cAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaag                                    >  2:593392/1‑128 (MQ=255)                                                                                                           cAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGc                        >  1:211916/1‑140 (MQ=255)                                                                                                           cAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGc                        <  1:823152/140‑1 (MQ=255)                                                                                                           cAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGc                        >  1:644334/1‑140 (MQ=255)                                                                                                           cAGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGc                        >  1:665320/1‑140 (MQ=255)                                                                                                            aagTTCCAAAAAACCTAACCTTAATAGGCCGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCg                       <  2:1135697/138‑1 (MQ=255)                                                                                                            aGGTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCg                       <  2:157960/140‑1 (MQ=255)                                                                                                              gTTCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTg                     <  2:211916/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  2:743260/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  2:485630/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  1:470185/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   >  2:926167/1‑140 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  1:598516/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  2:318773/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  2:24907/140‑1 (MQ=255)                                                                                                                tCCAGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  1:926167/140‑1 (MQ=255)                                                                                                                   aGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   <  2:255626/137‑1 (MQ=255)                                                                                                                   aGAAAACTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                   >  1:255626/1‑137 (MQ=255)                                                                                                                        aCTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCt           >  2:454529/1‑140 (MQ=255)                                                                                                                        aCTCAACGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCt           >  1:232833/1‑140 (MQ=255)                                                                                                                            aaCGTTATTAGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATc       >  1:653246/1‑140 (MQ=255)                                                                                                                                ttattaGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGAcc   <  2:354282/140‑1 (MQ=255)                                                                                                                                 tattaGACAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCa  >  1:983724/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCA  >  NC_000913/122865‑123132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑