Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,236,484	A→G	H407R (CAC→CGC)	preA →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,236,484	0	A	G	100.0%	241.7 / NA	66	H407R (CAC→CGC)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/36);  total (30/36)

ATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGC  >  NC_000913/2236348‑2236619                                                                                                                                         |                                                                                                                                        atggaatggaGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGccc                                                                                                                                      >  1:760831/1‑140 (MQ=255)     aatggaGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTa                                                                                                                                  <  1:88740/140‑1 (MQ=255)      atggaGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTaa                                                                                                                                 <  1:952510/140‑1 (MQ=255)      atggaGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTaa                                                                                                                                 <  2:410227/140‑1 (MQ=255)        ggaGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACg                                                                                                                               >  2:883621/1‑140 (MQ=255)        ggaGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACg                                                                                                                               <  2:166066/140‑1 (MQ=255)            cGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGt                                                                                                                           >  2:666564/1‑140 (MQ=255)            cGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGt                                                                                                                           <  1:652916/140‑1 (MQ=255)            cGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGt                                                                                                                           <  2:1058752/140‑1 (MQ=255)              agaAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTaa                                                                                                                         >  2:604272/1‑140 (MQ=255)              agaAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTaa                                                                                                                         >  1:96889/1‑140 (MQ=255)              agaAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTaa                                                                                                                         <  2:122920/140‑1 (MQ=255)                            ccGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCAt                                                                                                           >  2:401777/1‑140 (MQ=255)                             cGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATc                                                                                                          >  1:454898/1‑140 (MQ=255)                                aTTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCgg                                                                                                       <  1:604272/140‑1 (MQ=255)                                       tACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTgg                                                                                                >  1:462512/1‑140 (MQ=255)                                       tACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTgg                                                                                                >  2:824242/1‑140 (MQ=255)                                            agaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGTCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCt                                                                                           >  2:1107519/1‑140 (MQ=255)                                            agaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCt                                                                                           >  2:24779/1‑140 (MQ=255)                                            agaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCt                                                                                           >  1:693420/1‑140 (MQ=255)                                            agaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCt                                                                                           >  2:402649/1‑140 (MQ=255)                                            agaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCt                                                                                           >  1:401910/1‑140 (MQ=255)                                            agaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCt                                                                                           >  1:1441/1‑140 (MQ=255)                                              aaaTGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGt                                                                                         >  2:1053911/1‑140 (MQ=255)                                                     tgGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGa                                                                                  <  2:1079332/140‑1 (MQ=255)                                                      gggTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGAt                                                                                 <  2:43097/140‑1 (MQ=255)                                                        gTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGc                                                                               >  1:512886/1‑140 (MQ=255)                                                           gTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGc                                                                            <  1:1007914/140‑1 (MQ=255)                                                           gTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGc                                                                            >  2:1007914/1‑140 (MQ=255)                                                            tCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTc                                                                                             >  2:512056/1‑122 (MQ=255)                                                            tCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTc                                                                                             <  1:512056/122‑1 (MQ=255)                                                            tCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCt                                                                           >  1:883953/1‑140 (MQ=255)                                                               gCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGa                                                                        <  2:883953/140‑1 (MQ=255)                                                                 tttGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAAc                                                                      <  1:402649/140‑1 (MQ=255)                                                                    gtgGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGcc                                                                   <  2:379989/140‑1 (MQ=255)                                                                     tgGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCt                                                                  >  1:369856/1‑140 (MQ=255)                                                                           cGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCg                                                            <  2:104835/140‑1 (MQ=255)                                                                           cGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCg                                                            <  2:401910/140‑1 (MQ=255)                                                                           cGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCg                                                            <  2:369856/140‑1 (MQ=255)                                                                                gCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTa                                                       <  2:462512/140‑1 (MQ=255)                                                                                gCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTa                                                       <  1:62328/140‑1 (MQ=255)                                                                                  ccGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACa                                                     >  1:998966/1‑140 (MQ=255)                                                                                  ccGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACa                                                     <  2:96889/140‑1 (MQ=255)                                                                                   cGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACaa                                                    <  2:512886/140‑1 (MQ=255)                                                                                   cGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACaa                                                    <  1:431118/140‑1 (MQ=255)                                                                                     gTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACaaaa                                                  <  1:824242/140‑1 (MQ=255)                                                                                      tGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAGAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCAGTCGCCGGATGCGGCTTGAACGCCTTATCCGGc                                                          >  2:796928/1‑131 (MQ=255)                                                                                      tGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGc                                                          <  1:796928/131‑1 (MQ=255)                                                                                          ttGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATa                                             <  2:760831/140‑1 (MQ=255)                                                                                           tGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATAc                                            >  1:772903/1‑140 (MQ=255)                                                                                            gTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACa                                           <  1:401777/140‑1 (MQ=255)                                                                                              aTTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACaaa                                         <  2:454898/140‑1 (MQ=255)                                                                                                 gAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCa                                      <  2:998966/140‑1 (MQ=255)                                                                                                   gCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGt                                    <  1:259081/140‑1 (MQ=255)                                                                                                       ggggAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGt                                >  2:35840/1‑140 (MQ=255)                                                                                                        gggAAGTGAAGTTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGtt                               <  1:666564/140‑1 (MQ=255)                                                                                                            agtgaagtTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGc                           <  2:117191/140‑1 (MQ=255)                                                                                                             gtgaagtTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCt                          >  2:526841/1‑140 (MQ=255)                                                                                                              tgaagtTTAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCtt                         >  1:870667/1‑140 (MQ=255)                                                                                                                     tAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGt                                    >  2:73690/1‑122 (MQ=255)                                                                                                                     tAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGt                                    <  2:30490/122‑1 (MQ=255)                                                                                                                     tAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGt                                    >  1:30490/1‑122 (MQ=255)                                                                                                                     tAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGt                                    <  1:73690/122‑1 (MQ=255)                                                                                                                     tAAGAAAGGCGAGAAAGAACGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAg                  >  1:933945/1‑140 (MQ=255)                                                                                                                                     gaaCGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACgcgc  <  1:883621/140‑1 (MQ=255)                                                                                                                                     gaaCGCCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACgcgc  <  2:870667/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGC  >  NC_000913/2236348‑2236619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑