Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,267,029	T→C	V262A (GTG→GCG)	yeiQ →	putative dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,029	0	T	C	100.0%	256.2 / NA	71	V262A (GTG→GCG)	yeiQ	putative dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/34);  total (37/34)

CACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTT  >  NC_000913/2266893‑2267163                                                                                                                                         |                                                                                                                                       cACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGa                                                                                                                                     >  1:174655/1‑140 (MQ=255)  aCGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGaa                                                                                                                                    >  1:770404/1‑140 (MQ=255)    gTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGa                                                                                                                                  >  2:176855/1‑140 (MQ=255)         ttttCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACt                                                                                                                             >  1:894049/1‑140 (MQ=255)              cGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTtcgtc                                                                                                                        <  1:741291/140‑1 (MQ=255)              cGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTtcgtc                                                                                                                        >  1:422641/1‑140 (MQ=255)                       tGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGt                                                                                                               <  1:462017/140‑1 (MQ=255)                        ggTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTc                                                                                                              <  2:676115/140‑1 (MQ=255)                        ggTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTc                                                                                                              >  1:327446/1‑140 (MQ=255)                        ggTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTc                                                                                                              <  2:492157/140‑1 (MQ=255)                             aCCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTcctgcc                                                                                                         >  1:195111/1‑140 (MQ=255)                              ccGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGTGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTcctgcct                                                                                                        >  2:1067625/1‑140 (MQ=255)                                gCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTgg                                                                                                      <  2:1063535/140‑1 (MQ=255)                                       tCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg                                                                                               <  2:402210/140‑1 (MQ=255)                                        ccGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc                                                                                              <  2:174655/140‑1 (MQ=255)                                        ccGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc                                                                                              <  1:104791/140‑1 (MQ=255)                                           gCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGTGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAgg                                                                                           <  1:1067625/140‑1 (MQ=255)                                                aaCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc                                                                                              <  1:96614/132‑1 (MQ=255)                                                aaCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc                                                                                              >  2:96614/1‑132 (MQ=255)                                                aaCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                                                      >  2:360576/1‑140 (MQ=255)                                                    gacgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAAt                                                                                  <  1:27898/140‑1 (MQ=255)                                                     acgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATg                                                                                 >  2:355057/1‑140 (MQ=255)                                                          tCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATgg                                                                                <  2:624203/136‑1 (MQ=255)                                                          tCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATgg                                                                                >  1:624203/1‑136 (MQ=255)                                                          tCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGaa                                                                            <  2:327446/140‑1 (MQ=255)                                                                 tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTc                                                                     <  1:841696/140‑1 (MQ=255)                                                                 tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTc                                                                     <  2:475350/140‑1 (MQ=255)                                                                 tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTc                                                                     <  2:708651/140‑1 (MQ=255)                                                                   gAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCt                                                                   >  1:410455/1‑140 (MQ=255)                                                                   gAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCt                                                                   >  2:98780/1‑140 (MQ=255)                                                                   gAAATCAGCCAGCATCTGGGAGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCt                                                                   <  1:861757/140‑1 (MQ=255)                                                                        cagccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAt                                                              >  1:997483/1‑140 (MQ=255)                                                                          gccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATgg                                                            >  2:54001/1‑140 (MQ=255)                                                                            cagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGa                                                          <  1:360576/140‑1 (MQ=255)                                                                            cagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGa                                                          <  1:54001/140‑1 (MQ=255)                                                                                    gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGcc                                                                <  1:695504/126‑1 (MQ=255)                                                                                    gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGcc                                                                >  2:695504/1‑126 (MQ=255)                                                                                     ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAag                                                        >  1:308697/1‑133 (MQ=255)                                                                                     ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAag                                                        <  2:308697/133‑1 (MQ=255)                                                                                           aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg                                           >  2:462955/1‑140 (MQ=255)                                                                                           aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg                                           <  1:176855/140‑1 (MQ=255)                                                                                            aTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCgg                                          >  1:5457/1‑140 (MQ=255)                                                                                             tGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGa                                         <  1:462955/140‑1 (MQ=255)                                                                                               aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATg                                       >  2:660324/1‑140 (MQ=255)                                                                                                  ccTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCtt                                    >  2:670770/1‑140 (MQ=255)                                                                                                  ccTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCtt                                    >  2:1056612/1‑140 (MQ=255)                                                                                                    tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGTCATGGGAAGAGATGAAACTGCgg                                          <  2:781604/132‑1 (MQ=255)                                                                                                    tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGTCATGGGAAGAGATGAAACTGCgg                                          >  1:781604/1‑132 (MQ=255)                                                                                                     gcgcGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAAt                                 >  2:281520/1‑140 (MQ=255)                                                                                                     gcgcGATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAAt                                 >  1:554746/1‑140 (MQ=255)                                                                                                      cgcgATTAGCTTCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg                                           >  1:762659/1‑129 (MQ=255)                                                                                                      cgcgATTAGCTTCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg                                           <  2:762659/129‑1 (MQ=255)                                                                                                      cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg                                >  1:593895/1‑140 (MQ=255)                                                                                                       gcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg                                           <  1:371964/128‑1 (MQ=255)                                                                                                       gcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg                                           >  2:371964/1‑128 (MQ=255)                                                                                                       gcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg                               >  2:1086837/1‑140 (MQ=255)                                                                                                          aTTAGCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAg                            <  2:629644/140‑1 (MQ=255)                                                                                                              gCTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGccgc                        <  2:195111/140‑3 (MQ=255)                                                                                                               cTGCGAACCGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCAct                       <  1:660324/140‑1 (MQ=255)                                                                                                                       cGTTTATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtctc               <  2:593895/140‑1 (MQ=255)                                                                                                                         tttATCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGc             >  2:915049/1‑140 (MQ=255)                                                                                                                            aTCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa          <  1:915049/140‑1 (MQ=255)                                                                                                                             tCCAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTAt         <  1:1056612/140‑1 (MQ=255)                                                                                                                              ccAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATc        <  2:5457/140‑1 (MQ=255)                                                                                                                              ccAGTGGGTGGCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATc        <  2:894049/140‑1 (MQ=255)                                                                                                                                 gtgtgtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg     >  1:138794/1‑140 (MQ=255)                                                                                                                                 gtgggtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg              >  1:454327/1‑131 (MQ=255)                                                                                                                                 gtgggtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg              <  2:454327/131‑1 (MQ=255)                                                                                                                                 gtgggtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg     >  1:364883/1‑140 (MQ=255)                                                                                                                                 gtgggtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg     >  1:113580/1‑140 (MQ=255)                                                                                                                                  tgggtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg    >  1:545379/1‑140 (MQ=255)                                                                                                                                  tgggtggCGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg    >  2:647404/1‑140 (MQ=255)                                                                                                                                    ggtggcGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGtt  >  2:659926/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTT  >  NC_000913/2266893‑2267163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑