Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,598	T→C	R433R (CGT→CGC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,598	0	T	C	100.0%	252.2 / NA	68	R433R (CGT→CGC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

GGCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAAC  >  NC_000913/2277461‑2277728                                                                                                                                          |                                                                                                                                   ggCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCAt                                                                                                                                  >  1:599517/1‑140 (MQ=255) ggCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCAt                                                                                                                                  >  2:756698/1‑140 (MQ=255)   cTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTg                                                                                                                                >  1:516636/1‑140 (MQ=255)     tACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCg                                                                                                                              <  2:1040842/140‑1 (MQ=255)            tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGcc                                                                                                                        <  2:879184/139‑1 (MQ=255)            tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGcc                                                                                                                        >  1:879184/1‑139 (MQ=255)            tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCa                                                                                                                       >  1:1019838/1‑140 (MQ=255)             cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAg                                                                                                                      <  2:1019838/140‑1 (MQ=255)             cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAg                                                                                                                      >  2:725582/1‑140 (MQ=255)             cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAg                                                                                                                      >  1:923242/1‑140 (MQ=255)              aCCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAgg                                                                                                                     >  2:306206/1‑140 (MQ=255)                   ccGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCAtt                                                                                                                <  1:871620/140‑1 (MQ=255)                    cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                               >  2:572969/1‑140 (MQ=255)                    cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                               >  2:1042729/1‑140 (MQ=255)                    cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                               <  1:164425/140‑1 (MQ=255)                        gCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTc                                                                                                           >  2:658207/1‑140 (MQ=255)                           ttCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTa                                                                                                        <  1:124676/140‑1 (MQ=255)                             cTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAg                                                                                                      <  1:241516/140‑1 (MQ=255)                              tGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGc                                                                                                     <  2:516636/140‑1 (MQ=255)                               gCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGcc                                                                                                    <  1:572969/140‑1 (MQ=255)                                   cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                <  2:599517/140‑1 (MQ=255)                                   cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                <  1:658207/140‑1 (MQ=255)                                     caACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCt                                                                                              >  2:641161/1‑140 (MQ=255)                                       aCGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGa                                                                                            <  2:921113/140‑1 (MQ=255)                                        cgcgAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGAt                                                                                           <  2:519218/140‑1 (MQ=255)                                                  aaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtgat                                                                                 >  1:151325/1‑140 (MQ=255)                                                  aaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtgat                                                                                 <  1:1042729/140‑1 (MQ=255)                                                     tGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGaa                                                                              <  2:1000604/140‑1 (MQ=255)                                                       aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                            >  1:131094/1‑140 (MQ=255)                                                           ccGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAca                                                                        >  2:306175/1‑140 (MQ=255)                                                           ccGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAca                                                                        >  2:175070/1‑140 (MQ=255)                                                            cGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcat                                                                       <  1:35952/140‑1 (MQ=255)                                                             gTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatc                                                                      >  1:729100/1‑140 (MQ=255)                                                               gATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatcat                                                                    >  2:987667/1‑140 (MQ=255)                                                               gATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatcat                                                                    <  1:175070/140‑1 (MQ=255)                                                               gATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatcat                                                                    <  2:1050382/140‑1 (MQ=255)                                                                   cATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACt                                                                <  1:977700/140‑1 (MQ=255)                                                                         gTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaa                                                          <  2:729100/140‑1 (MQ=255)                                                                           gggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                        <  1:965714/140‑1 (MQ=255)                                                                           gggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                        <  2:151325/140‑1 (MQ=255)                                                                            ggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAAc                                                       >  2:1128732/1‑140 (MQ=255)                                                                             gATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACg                                                      >  1:892495/1‑140 (MQ=255)                                                                                  gATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                                 <  1:641161/140‑1 (MQ=255)                                                                                  gATCCTGAAACACGCCACCGTTATCCAGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                                 <  1:756698/140‑1 (MQ=255)                                                                                       tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTAc                                                  >  1:822041/1‑134 (MQ=255)                                                                                       tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTAc                                                  <  2:822041/134‑1 (MQ=255)                                                                                       tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                            >  2:299493/1‑140 (MQ=255)                                                                                       tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                            >  2:414752/1‑140 (MQ=255)                                                                                       tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                            >  2:516213/1‑140 (MQ=255)                                                                                        gAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCa                                           >  2:949042/1‑140 (MQ=255)                                                                                             acGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAatat                                      >  1:948130/1‑140 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                   >  2:331621/1‑126 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                   <  1:331621/126‑1 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                     >  2:162639/1‑140 (MQ=255)                                                                                               gCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTg                                    >  2:180934/1‑140 (MQ=255)                                                                                                ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                   <  1:306175/140‑1 (MQ=255)                                                                                                  aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                 <  1:390787/140‑1 (MQ=255)                                                                                                  aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                 <  1:1128732/140‑1 (MQ=255)                                                                                                      ttATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                     >  2:774005/1‑132 (MQ=255)                                                                                                      ttATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                     <  1:774005/132‑1 (MQ=255)                                                                                                                      tctGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGcaaca             <  1:180934/140‑1 (MQ=255)                                                                                                                           tggtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGc        <  1:299493/140‑1 (MQ=255)                                                                                                                           tggtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGc        <  2:892495/140‑1 (MQ=255)                                                                                                                           tggtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGc        >  1:553683/1‑140 (MQ=255)                                                                                                                             gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt      >  2:639298/1‑140 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa   >  1:371551/1‑140 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa   >  1:45167/1‑140 (MQ=255)                                                                                                                                 gCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAAc  <  2:371551/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   GGCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAAC  >  NC_000913/2277461‑2277728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑