Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,411,136	T→C	T73A (ACC→GCC)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,136	0	T	C	100.0%	262.9 / NA	71	T73A (ACC→GCC)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/36);  total (35/36)

AAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCG  >  NC_000913/2411000‑2411270                                                                                                                                         |                                                                                                                                       aaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACg                                                                                                                                     >  1:522553/1‑140 (MQ=255)    aTAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGAc                                                                                                                                  <  2:985216/140‑1 (MQ=255)      aGCGACAACGCCGGTTGAACTGATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCa                                                                                                                                >  2:820899/1‑140 (MQ=255)      aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCa                                                                                                                                >  1:590502/1‑140 (MQ=255)      aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCa                                                                                                                                >  2:870359/1‑140 (MQ=255)       gCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCaa                                                                                                                               >  1:53365/1‑140 (MQ=255)        cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCaaa                                                                                                                              >  2:818819/1‑140 (MQ=255)         gACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAAc                                                                                                                             >  2:354980/1‑140 (MQ=255)           cAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCa                                                                                                                           >  1:1067712/1‑140 (MQ=255)              cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCg                                                                                                                        >  2:988246/1‑140 (MQ=255)               gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGc                                                                                                                       <  1:776718/140‑1 (MQ=255)               gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGc                                                                                                                       <  2:248653/140‑1 (MQ=255)               gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGc                                                                                                                       <  2:590502/140‑1 (MQ=255)               gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGc                                                                                                                       <  1:1106060/140‑1 (MQ=255)                   gTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCaat                                                                                                                   >  1:400754/1‑140 (MQ=255)                         cTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATaaa                                                                                                             <  1:137716/140‑1 (MQ=255)                          tCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAAc                                                                                                            <  2:738432/140‑1 (MQ=255)                           cATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACa                                                                                                           <  2:52909/140‑1 (MQ=255)                             tAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAg                                                                                                         >  2:213150/1‑140 (MQ=255)                                aCGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAggcg                                                                                                      <  2:53365/140‑1 (MQ=255)                                aCGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAggcg                                                                                                      <  1:915429/140‑1 (MQ=255)                                aCGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAggcg                                                                                                      <  1:818819/140‑1 (MQ=255)                                aCGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAggcg                                                                                                      <  2:995358/140‑1 (MQ=255)                                 cgcgCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAggcgg                                                                                                     >  2:1057537/1‑140 (MQ=255)                                    gcCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa                                                                                                  >  1:66304/1‑140 (MQ=255)                                    gcCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa                                                                                                  >  2:83434/1‑140 (MQ=255)                                     ccAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGCTTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAt                                                                                                 <  2:1067712/140‑1 (MQ=255)                                     ccAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACAAAACCATCGCCAATAATAAACAAGGCGGCAAt                                                                                                 <  1:229810/140‑1 (MQ=255)                                          ccccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa                                                                                            <  1:884951/140‑1 (MQ=255)                                          ccccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa                                                                                            <  2:522553/140‑1 (MQ=255)                                          ccccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa                                                                                            <  1:213150/140‑1 (MQ=255)                                          ccccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa                                                                                            <  2:592319/140‑1 (MQ=255)                                          ccccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa                                                                                            <  1:79437/140‑1 (MQ=255)                                          ccccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa                                                                                            <  2:976832/140‑1 (MQ=255)                                           cccGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc                                                                                           <  1:641352/140‑1 (MQ=255)                                              gcgACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa                                                                                          >  1:167220/1‑138 (MQ=255)                                              gcgACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa                                                                                          <  2:167220/138‑1 (MQ=255)                                              gcgACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAAc                                                                                        >  2:241376/1‑140 (MQ=255)                                                gACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGt                                                                                      <  1:589062/140‑1 (MQ=255)                                                gACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGt                                                                                      <  2:400754/140‑1 (MQ=255)                                                 aCGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGtt                                                                                     <  1:1057537/140‑1 (MQ=255)                                                    gTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAGACAAGGCGGCAATCAGGACAACGTTAgg                                                                                  >  1:940365/1‑140 (MQ=255)                                                    gTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAgg                                                                                  >  2:801041/1‑140 (MQ=255)                                                            caacaaAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGaaa                                                                          >  2:315398/1‑140 (MQ=255)                                                              acaaAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAg                                                                        >  1:5498/1‑140 (MQ=255)                                                                 aaaCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCg                                                                     <  1:801041/140‑1 (MQ=255)                                                                   aCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGa                                                                   >  2:658159/1‑140 (MQ=255)                                                                   aCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGa                                                                   >  1:967209/1‑140 (MQ=255)                                                                      aaCATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGaaaa                                                                <  2:967209/140‑1 (MQ=255)                                                                       aCATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAAt                                                               <  1:400789/140‑1 (MQ=255)                                                                           tttGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACtt                                                           <  1:870359/140‑1 (MQ=255)                                                                                  tCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGAc                                                    >  2:922720/1‑140 (MQ=255)                                                                                       aTTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCa                                               <  1:315398/140‑1 (MQ=255)                                                                                         tGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAATAACTTTTGGGCCttccacc                                             >  1:207611/1‑134 (MQ=255)                                                                                          gCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCg                                            <  1:241376/140‑1 (MQ=255)                                                                                          gCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCg                                            <  1:449161/140‑1 (MQ=255)                                                                                           cccGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAGACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGt                                           <  2:940365/140‑1 (MQ=255)                                                                                           cccGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGt                                           >  1:788524/1‑140 (MQ=255)                                                                                             cGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTc                                         >  1:151882/1‑140 (MQ=255)                                                                                                aaCTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGc                                      >  2:501772/1‑140 (MQ=255)                                                                                                            ccATGTTCCCATTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAcg                          >  2:820087/1‑140 (MQ=255)                                                                                                                      aTTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaac                >  2:635152/1‑140 (MQ=255)                                                                                                                      aTTACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaac                >  1:978574/1‑140 (MQ=255)                                                                                                                       ttACGGTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca               <  2:507708/140‑1 (MQ=255)                                                                                                                           ggTGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaacaaaca           >  1:643632/1‑140 (MQ=255)                                                                                                                             tGGCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAAcaaacaaa         >  1:174395/1‑140 (MQ=255)                                                                                                                               gCAACACCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGc       >  1:1126840/1‑140 (MQ=255)                                                                                                                                   cacCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAcc   <  1:83434/140‑1 (MQ=255)                                                                                                                                    acCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAccc  >  2:731839/1‑139 (MQ=255)                                                                                                                                    acCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAc    >  1:1098572/1‑138 (MQ=255)                                                                                                                                    acCGGCACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAc    <  2:1098572/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCG  >  NC_000913/2411000‑2411270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑