Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	200,441	A→G	intergenic (+81/‑41)	bamA → / → skp	outer membrane protein assembly factor BamA/periplasmic chaperone Skp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	200,441	0	A	G	100.0%	211.9 / NA	59	intergenic (+81/‑41)	bamA/skp	outer membrane protein assembly factor BamA/periplasmic chaperone Skp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/27);  total (32/27)

GAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAGCCTG  >  NC_000913/200314‑200577                                                                                                                                |                                                                                                                                         gagaCAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCg                                                                                                                              <  2:950561/140‑1 (MQ=255)  agaCAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCgg                                                                                                                             >  2:67439/1‑140 (MQ=255)            gAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATggg                                                                                                                   <  2:685871/140‑1 (MQ=255)            gAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATggg                                                                                                                   <  2:618736/140‑1 (MQ=255)             aaCAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGa                                                                                                                  <  1:24040/140‑1 (MQ=255)              aCAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGAt                                                                                                                 >  2:1141622/1‑140 (MQ=255)                 gTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGt                                                                                                              <  2:280895/140‑1 (MQ=255)                  ttCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTa                                                                                                             >  2:1158090/1‑140 (MQ=255)                  ttCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTa                                                                                                             >  1:353363/1‑140 (MQ=255)                                cGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATtgtg                                                                                               >  2:283956/1‑140 (MQ=255)                                cGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATtgtg                                                                                               <  1:669098/140‑1 (MQ=255)                                      aaCCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAg                                                                                         <  1:213127/140‑1 (MQ=255)                                      aaCCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAg                                                                                         <  1:993814/140‑1 (MQ=255)                                           ggTAAGTGTTCTCCACAAAGGAGTGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTgg                                                                                      <  2:379138/138‑1 (MQ=255)                                           ggTAAGTGTTCTCCACAAAGGAGTGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTgg                                                                                      >  1:379138/1‑138 (MQ=255)                                           ggTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGtt                                                                                    >  1:620600/1‑140 (MQ=255)                                            gTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGtta                                                                                   <  1:314485/140‑1 (MQ=255)                                             tAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGttat                                                                                  <  1:283956/140‑1 (MQ=255)                                                        cacaAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCCCCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGt                                                                       <  1:279544/140‑1 (MQ=255)                                                        cacaAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGt                                                                       <  2:164870/140‑1 (MQ=255)                                                          caAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGtct                                                                     <  2:353363/140‑1 (MQ=255)                                                                    tagtggtagtgTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCa                                                           <  1:67439/140‑1 (MQ=255)                                                                         gtagtgTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      <  1:929563/140‑1 (MQ=255)                                                                         gtagtgTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  2:954960/1‑140 (MQ=255)                                                                         gtagtgTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  2:859568/1‑140 (MQ=255)                                                                         gtagtgTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  2:315566/1‑140 (MQ=255)                                                                         gtagtgTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGAGGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  2:654067/1‑140 (MQ=255)                                                                            gtgtAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  1:156915/1‑137 (MQ=255)                                                                            gtgtAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      <  2:156915/137‑1 (MQ=255)                                                                             tgtAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      <  2:526363/136‑1 (MQ=255)                                                                             tgtAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  1:526363/1‑136 (MQ=255)                                                                              gtAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACtt                                                 >  2:407537/1‑140 (MQ=255)                                                                              gtAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACtt                                                 >  1:622957/1‑140 (MQ=255)                                                                                 gCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      >  2:548230/1‑132 (MQ=255)                                                                                 gCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGc                                                      <  1:548230/132‑1 (MQ=255)                                                                                 gCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTg                                              >  2:852394/1‑140 (MQ=255)                                                                                  cGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGc                                             >  1:802628/1‑140 (MQ=255)                                                                                         tttAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAggcg                                      <  1:852394/140‑1 (MQ=255)                                                                                                 aTCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACaa                              >  2:1069240/1‑140 (MQ=255)                                                                                                 aTCAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACaa                              >  1:144403/1‑140 (MQ=255)                                                                                                   cAATATAAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACaaaa                            >  1:1036714/1‑140 (MQ=255)                                                                                                      tataAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTCATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTg                         <  2:802628/140‑1 (MQ=255)                                                                                                       ataAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGc                        <  1:1141622/140‑1 (MQ=255)                                                                                                        taAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCa                       >  1:1062762/1‑140 (MQ=255)                                                                                                        taAGATCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCa                       <  1:407537/140‑1 (MQ=255)                                                                                                            aTCGCCGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCg                   >  2:521395/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCaa               >  2:1138324/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCaa               >  2:1105751/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCaa               >  1:819406/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCaa               >  2:784434/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCaa               >  2:764526/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCaa               >  1:316158/1‑140 (MQ=255)                                                                                                                ccGGGCCACGCAAAGAGCTGCACCCTCCGGAGCAAATGGGACGGCAAGGAGGTTATTGGGAAAAAGAGCTTAGTAGCTGCAGTTCTCGGTTTAGCACTGGCAACTTCTGCTCAGCCGGCTGACAAAATTGCAATCGTCaa               >  2:1115591/1‑140 (MQ=255)                                                                                                                   ggCCACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAt            >  1:965411/1‑140 (MQ=255)                                                                                                                      cACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATggg         <  1:784434/140‑1 (MQ=255)                                                                                                                      cACGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATggg         <  1:1105751/140‑1 (MQ=255)                                                                                                                        cGCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCa       >  2:446543/1‑140 (MQ=255)                                                                                                                         gCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAg      <  1:521395/140‑1 (MQ=255)                                                                                                                         gCAAAGAGCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAACCGTCAACATGGGCAg      <  2:620600/140‑1 (MQ=255)                                                                                                                             agagCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAGCCTg  <  1:446543/140‑1 (MQ=255)                                                                                                                             agagCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAGCCTg  <  2:819406/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         GAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAGCCTG  >  NC_000913/200314‑200577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑