Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,577,609	C→T	A257T (GCG→ACG)	maeB ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,577,609	0	C	T	100.0%	149.2 / NA	42	A257T (GCG→ACG)	maeB	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/28);  total (14/28)

CGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGC  >  NC_000913/2577473‑2577745                                                                                                                                         |                                                                                                                                         cGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGcc                                                                                                                                       <  1:632716/140‑1 (MQ=255)             gCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCAcatc                                                                                                                           <  2:180858/140‑1 (MQ=255)              ccAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCAcatca                                                                                                                          <  1:961609/140‑1 (MQ=255)               cAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCTGTGCCTTCAATCAcatcat                                                                                                                         <  2:1156462/140‑1 (MQ=255)                                ccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAg                                                                                                                     >  2:997528/1‑127 (MQ=255)                                ccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAg                                                                                                                     <  1:997528/127‑1 (MQ=255)                                ccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCg                                                                                                        <  2:945352/140‑1 (MQ=255)                                  ggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTc                                                                                                      <  2:236284/140‑1 (MQ=255)                                     tccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATc                                                                                                   <  2:757289/140‑1 (MQ=255)                                                      cagGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCgg                                                                                  <  2:47978/140‑1 (MQ=255)                                                             aTTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGt                                                                           <  1:1116945/140‑1 (MQ=255)                                                             aTTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGt                                                                           <  1:580538/140‑1 (MQ=255)                                                              ttGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGtt                                                                          >  1:274916/1‑140 (MQ=255)                                                                ggCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTc                                                                        >  1:500288/1‑140 (MQ=255)                                                                  cgcACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATg                                                                                     <  1:50218/125‑1 (MQ=255)                                                                  cgcACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATg                                                                                     >  2:50218/1‑125 (MQ=255)                                                                   gcACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGc                                                                     >  1:637502/1‑140 (MQ=255)                                                                               ttcttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTc                                                         >  2:65037/1‑140 (MQ=255)                                                                                tcttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                                        <  1:237905/140‑1 (MQ=255)                                                                                  ttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACg                                                      <  1:577963/140‑1 (MQ=255)                                                                                   tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  2:787355/140‑1 (MQ=255)                                                                                   tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  2:717466/140‑1 (MQ=255)                                                                                   tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  2:500288/140‑1 (MQ=255)                                                                                   tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  1:367073/140‑1 (MQ=255)                                                                                   tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  2:278072/140‑1 (MQ=255)                                                                                   tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTCTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  2:23069/140‑1 (MQ=255)                                                                                     aCCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGcc                                                   <  2:637502/140‑1 (MQ=255)                                                                                          ttCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                                        >  2:117260/1‑130 (MQ=255)                                                                                          ttCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                                        <  1:117260/130‑1 (MQ=255)                                                                                              tGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTgatagat                                          <  2:958408/140‑1 (MQ=255)                                                                                               gggTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     <  1:1124434/128‑1 (MQ=255)                                                                                               gggTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                                                     >  2:1124434/1‑128 (MQ=255)                                                                                                    aGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGcc                                    >  2:35955/1‑140 (MQ=255)                                                                                                    aGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGcc                                    >  1:116048/1‑140 (MQ=255)                                                                                                           tCGGGCCGGAACAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGaa                             >  1:31173/1‑140 (MQ=255)                                                                                                                     aCAGCCCAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCa                   <  2:490143/140‑1 (MQ=255)                                                                                                                         cccAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGat               >  2:739783/1‑140 (MQ=255)                                                                                                                         cccAGGAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGat               >  1:349742/1‑140 (MQ=255)                                                                                                                              gAAAATATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGatgttat          >  2:850973/1‑140 (MQ=255)                                                                                                                               aaaaTATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGatgttatg         <  1:65037/140‑1 (MQ=255)                                                                                                                               aaaaTATCCGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGatgttatg         <  1:500117/140‑1 (MQ=255)                                                                                                                                      ccGTGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGc  <  1:739783/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGC  >  NC_000913/2577473‑2577745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑