Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,604,329	T→C	G161G (GGT→GGC)	hyfC →	hydrogenase 4 component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,604,329	0	T	C	100.0%	168.3 / NA	46	G161G (GGT→GGC)	hyfC	hydrogenase 4 component C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

TTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGTTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGATGTTG  >  NC_000913/2604194‑2604459                                                                                                                                        |                                                                                                                                   ttttttCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCa                                                                                                                                <  2:282965/140‑1 (MQ=255)  tttttCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCAc                                                                                                                               <  2:315762/140‑1 (MQ=255)            ctctTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCgag                                                                                                                     <  1:833045/140‑1 (MQ=255)            ctctTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCgag                                                                                                                     <  1:426262/140‑1 (MQ=255)             tctTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCgaga                                                                                                                    <  1:680398/140‑1 (MQ=255)               tttCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAgatg                                                                                                                  <  2:858277/140‑1 (MQ=255)               tttCCGGGCTGGATACCGGAAGTCCGTATGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAAGCTCCGCGCATTTAGAgatg                                                                                                                  <  2:1026007/140‑1 (MQ=255)                 tCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAgatgat                                                                                                                <  1:462265/140‑1 (MQ=255)                        tGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAAt                                                                                                         <  1:362163/140‑1 (MQ=255)                          gATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATAc                                                                                                       >  1:1143149/1‑140 (MQ=255)                           aTACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACg                                                                                                      >  1:28336/1‑140 (MQ=255)                               cGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTgg                                                                                                  >  1:859476/1‑140 (MQ=255)                                 gAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCg                                                                                                >  2:113783/1‑140 (MQ=255)                                 gAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCg                                                                                                <  2:771707/140‑1 (MQ=255)                                  aaGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGa                                                                                               <  2:166011/140‑1 (MQ=255)                                  aaGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGa                                                                                               <  1:113783/140‑1 (MQ=255)                                    gTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGc                                                                                                     >  2:508895/1‑132 (MQ=255)                                    gTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGc                                                                                                     <  1:508895/132‑1 (MQ=255)                                    gTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATg                                                                                             >  1:1000453/1‑140 (MQ=255)                                       cGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGc                                                                                          >  1:30427/1‑140 (MQ=255)                                            gCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGa                                                                                               >  2:24330/1‑130 (MQ=255)                                            gCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGa                                                                                               <  1:24330/130‑1 (MQ=255)                                            gCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGaa                                                                                     >  1:545813/1‑140 (MQ=255)                                              gggAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACt                                                                                   <  2:28336/140‑1 (MQ=255)                                                   tCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTcgccg                                                                              <  2:1143149/140‑1 (MQ=255)                                                     ggTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCt                                                                            >  2:256281/1‑140 (MQ=255)                                                        gCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAac                                                                         <  2:859476/140‑1 (MQ=255)                                                            gTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAaccacc                                                                     >  1:1095661/1‑140 (MQ=255)                                                            gTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAaccacc                                                                     >  1:442841/1‑140 (MQ=255)                                                             tCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAac                                                                         >  1:810080/1‑135 (MQ=255)                                                             tCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAac                                                                         <  2:810080/135‑1 (MQ=255)                                                                           tCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTg                                                      <  2:1000453/140‑1 (MQ=255)                                                                                     ggTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGtt                                            >  2:812124/1‑140 (MQ=255)                                                                                       tCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGtttt                                          >  2:244567/1‑140 (MQ=255)                                                                                         gAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGc                                        <  2:545813/140‑1 (MQ=255)                                                                                           aCCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGtttt                                          >  2:1108917/1‑136 (MQ=255)                                                                                           aCCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGtttt                                          <  1:1108917/136‑1 (MQ=255)                                                                                            ccAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTg                                     <  1:403396/140‑1 (MQ=255)                                                                                                     aTTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTga                            <  2:1095661/140‑1 (MQ=255)                                                                                                          ctcACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATgg                       >  1:697382/1‑140 (MQ=255)                                                                                                           tcACTGCTGGTATTGGCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATggg                      >  1:892503/1‑140 (MQ=255)                                                                                                                         ggCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGTGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTg        >  1:207031/1‑140 (MQ=255)                                                                                                                         ggCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTg        >  1:280726/1‑140 (MQ=255)                                                                                                                         ggCGCTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTg        >  1:479642/1‑140 (MQ=255)                                                                                                                           cgcTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGAt      >  2:212620/1‑140 (MQ=255)                                                                                                                           cgcTGATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGAt      >  1:71542/1‑140 (MQ=255)                                                                                                                               gATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGATGTTg  >  2:45617/1‑140 (MQ=255)                                                                                                                               gATAGCAGGCTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGATGTTg  >  1:1040317/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   TTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGTTCCACGCATATCGAGATGATCAGCAATACGCTGGCGATGGGCTGGAACTCGCCGCTAACCACCGTACTGGCGTTACTGGCCTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGATGTTG  >  NC_000913/2604194‑2604459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑