Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,613,047	T→C	V383A (GTG→GCG)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,613,047	0	T	C	100.0%	162.9 / NA	45	V383A (GTG→GCG)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/13);  total (32/13)

GCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGTGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGAT  >  NC_000913/2612910‑2613182                                                                                                                                           |                                                                                                                                          gcTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGa                                                                                                                                         >  2:292606/1‑140 (MQ=255)  cTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGAt                                                                                                                                        <  2:941187/140‑1 (MQ=255)  cTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGAt                                                                                                                                        <  1:940700/140‑1 (MQ=255)          gAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCagag                                                                                                                                <  1:292606/140‑1 (MQ=255)           aaTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGc                                                                                                                               <  1:566795/140‑1 (MQ=255)               tGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGa                                                                                                                             >  1:855919/1‑138 (MQ=255)               tGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGa                                                                                                                             <  2:855919/138‑1 (MQ=255)               tGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATc                                                                                                                           >  2:693315/1‑140 (MQ=255)                 ggCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCa                                                                                                                         <  1:1111620/140‑1 (MQ=255)                      cGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAAc                                                                                                                   >  1:879521/1‑140 (MQ=255)                                atctatcAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCcgcg                                                                                                        >  2:1045656/1‑140 (MQ=255)                                atctatcAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCcgcg                                                                                                        >  1:526368/1‑140 (MQ=255)                                 tctatcAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCcgcga                                                                                                       >  1:969464/1‑140 (MQ=255)                                      cAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCcgcgacgcga                                                                                                  >  1:332227/1‑140 (MQ=255)                                         aGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACaa                                                                                               >  2:750058/1‑140 (MQ=255)                                           ccAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGc                                                                                             >  1:586804/1‑140 (MQ=255)                                           ccAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGc                                                                                             >  1:1064314/1‑140 (MQ=255)                                           ccAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGc                                                                                             >  1:768434/1‑140 (MQ=255)                                             aGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑GGCCCGCGACGCGACAAGCCg                                                                                           >  1:655093/1‑140 (MQ=255)                                             aGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCg                                                                                           <  1:1045656/140‑1 (MQ=255)                                             aGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCg                                                                                           <  1:693315/140‑1 (MQ=255)                                                        gACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGGCAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAAt                                                                                >  1:303869/1‑140 (MQ=255)                                                         aCCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATc                                                                               >  1:738907/1‑140 (MQ=255)                                                             gCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACt                                                                           >  1:892579/1‑140 (MQ=255)                                                              cTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTg                                                                          >  1:654674/1‑140 (MQ=255)                                                              cTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTg                                                                          >  1:201309/1‑140 (MQ=255)                                                               tCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTgc                                                                         <  2:13163/140‑1 (MQ=255)                                                                ccAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTgcg                                                                        <  2:1064314/140‑1 (MQ=255)                                                                   gcagGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTg                                                                     <  1:726251/140‑1 (MQ=255)                                                                   gcagGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTg                                                                     <  2:95060/140‑1 (MQ=255)                                                                   gcagGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTg                                                                     <  1:918157/140‑1 (MQ=255)                                                                        tAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATc                                                                >  1:34359/1‑140 (MQ=255)                                                                        tAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATc                                                                >  2:518669/1‑140 (MQ=255)                                                                               gggggagAAGAGCGACAGTGGGGTGTTG‑TTTGGGGGAAAACCGGAAACGGAAAAAGAGGCGACCCCAAGAGCAACCCA‑‑CAACTTCACCCCGCGCCGCAAAAGCCCGCTGGAAAAAATCACCGGCGCTGCCATCCCCGCCa                                                         <  2:705459/134‑1 (MQ=255)                                                                                 tgGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGt                                                       >  2:940272/1‑140 (MQ=255)                                                                                 tgGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGt                                                       >  2:955707/1‑140 (MQ=255)                                                                                 tgGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGt                                                       >  1:732794/1‑140 (MQ=255)                                                                                       agagCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTg                                                 >  1:705459/1‑140 (MQ=255)                                                                                             aCAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGt                                           >  2:723507/1‑140 (MQ=255)                                                                                                    ggTGTTGATTTGGGGAAAACCGGG‑AACGGGAAAGGGGGCGACCGCAAGAGCATCCCA‑CCATCTG‑GCCCGGCGAGGCAACAAGCCGCTGGAAAAAATCACCGGCGCTCCCCCCCCCGCCAGTCTTCTGGAAAGTGAGTTAt                                    <  2:655093/140‑1 (MQ=255)                                                                                                        ttGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCgg                                >  1:365791/1‑140 (MQ=255)                                                                                                         tGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGt                               >  1:372743/1‑140 (MQ=255)                                                                                                           aTTTGTGGTGAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCa                             >  1:992628/1‑140 (MQ=255)                                                                                                                tggtgAAACCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACa                        >  1:396371/1‑140 (MQ=255)                                                                                                                     aaaCCGG‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAggg                   >  2:493626/1‑140 (MQ=255)                                                                                                                          gg‑AACTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGtt              >  1:1044562/1‑140 (MQ=255)                                                                                                                              aCTGGCAAAGAGGCGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTAc           >  1:638184/1‑140 (MQ=255)                                                                                                                                    aaaGGGGCGACCGCAAGAGCAACCCA‑CCAACTC‑ACCCCGCGCCGCCAAACCCCGCTGGAAAAAATCACCGGCGCTCCCACCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGc     <  2:197605/140‑1 (MQ=255)                                                                                                                                       gagGCGATCGCCAGAGC‑CTCCACAAAACTC‑CGCCCGCGCCGCAAAAAGCCGGTGGTAAAAAACAACTGCGCTGCCACCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGAt  <  2:365791/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                          GCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTTGTGGTGAAACCGG‑AACTGGCAAAGAGGTGATCGCCAGAGCGATCCA‑TCAACTT‑AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGAT  >  NC_000913/2612910‑2613182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑