Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,668,030	T→C	E14E (GAA→GAG)  E289E (GAA→GAG)	iroK ← hcaR ←	protein IroK DNA‑binding transcriptional dual regulator HcaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,668,030	0	T	C	100.0%	143.1 / NA	40	E14E (GAA→GAG)  E289E (GAA→GAG)	iroK hcaR	protein IroK DNA‑binding transcriptional dual regulator HcaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

GTTGAAAAATAAGAATATTCGAGCCTGCTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGTTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTAAAATTATT  >  NC_000913/2667900‑2668161                                                                                                                                   |                                                                                                                                    gTTGAAAAATAAGAATATTCGAGCCTGCTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGcaca                                                                                                                            <  2:351277/140‑1 (MQ=255)            aGAATATTCGAGCCTGCTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGaa                                                                                                                 <  1:1084702/140‑1 (MQ=255)                  ttCGAGCCTGCTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATcgcg                                                                                                           >  1:588645/1‑140 (MQ=255)                        ccTGCTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGc                                                                                                     >  1:1132889/1‑140 (MQ=255)                            cTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGc                                                                                                 >  2:1056947/1‑140 (MQ=255)                               cccGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTc                                                                                              >  1:183168/1‑140 (MQ=255)                                              gCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTttcttc                                                                               >  1:799698/1‑140 (MQ=255)                                                cAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCa                                                                             >  1:374937/1‑140 (MQ=255)                                                  gTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACg                                                                           >  1:951241/1‑140 (MQ=255)                                                    tCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGcc                                                                         <  1:1056947/140‑1 (MQ=255)                                                    tCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGcc                                                                         <  2:366045/140‑1 (MQ=255)                                                         aGCACTAAGAATCTGAGATCCGCGTCGCATGTTGCAAGGATGATGCTTAATTCTGCAGTTTCGCTTGCCGATCGCTCTTGTTTTCTGGTGATGAGATCGCGTAGTGTCGGCTTCATCTCCCCTTTCTTCCACGCCTTctg                                                                    <  2:418380/140‑3 (MQ=255)                                                         aGCACTAAAAAACTGATATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAg                                                                    <  2:149028/140‑1 (MQ=255)                                                         aGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAg                                                                    <  2:374937/140‑1 (MQ=255)                                                         aGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAg                                                                    <  2:183168/140‑1 (MQ=255)                                                             cTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTttcttc                                                                               <  2:841112/125‑1 (MQ=255)                                                             cTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTttcttc                                                                               >  1:841112/1‑125 (MQ=255)                                                             cTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGc                                                                          >  2:34229/1‑130 (MQ=255)                                                             cTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGc                                                                          <  1:34229/130‑1 (MQ=255)                                                                    aCTGCGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATgg                                                         <  2:1064005/140‑1 (MQ=255)                                                                      tGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGt                                                                   <  2:858757/128‑1 (MQ=255)                                                                      tGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGt                                                                   >  1:858757/1‑128 (MQ=255)                                                                                   gCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATg                                                          >  2:348307/1‑124 (MQ=255)                                                                                   gCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATg                                                          <  1:348307/124‑1 (MQ=255)                                                                                    cATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGC‑TCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCa                                        >  1:1037224/1‑140 (MQ=255)                                                                                       gTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAAt                                      >  2:433554/1‑140 (MQ=255)                                                                                          gCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATggg                                   >  1:218378/1‑140 (MQ=255)                                                                                           cAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGa                                  <  1:433554/140‑1 (MQ=255)                                                                                            aaaaaTAATGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGAc                                 <  2:1132889/140‑1 (MQ=255)                                                                                                   atGCGTAATTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAaac                          >  2:817881/1‑140 (MQ=255)                                                                                                         aaTTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTg                    >  2:627219/1‑140 (MQ=255)                                                                                                          aTTATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGt                   >  1:388873/1‑140 (MQ=255)                                                                                                           ttATGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACTCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTc                  <  1:209907/140‑1 (MQ=255)                                                                                                              tGCCGTTACGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCgg               >  1:640928/1‑140 (MQ=255)                                                                                                                     aCGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGTCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTaaa        >  2:1074464/1‑140 (MQ=255)                                                                                                                     aCGCTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTaaa        >  2:699819/1‑140 (MQ=255)                                                                                                                        cTTGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTAAAatt     <  1:817881/140‑1 (MQ=255)                                                                                                                         ttGCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTAAAatta    >  2:323715/1‑140 (MQ=255)                                                                                                                           gCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTaaaa       <  2:394736/135‑1 (MQ=255)                                                                                                                           gCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTaaaa       >  1:394736/1‑135 (MQ=255)                                                                                                                           gCCAAACGCTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTAAAattatt  <  1:323715/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    GTTGAAAAATAAGAATATTCGAGCCTGCTTCCCGATGTCTGTAACGCCAGTTCACAAGCACTAAAAAACTGAGATCCGCGACGCATGTTGCAAAAATAATGCGTAATTATGCCGTTACGCTTGCCAAACGTTCCTGCACAATGGCGATGAAATCGCGTAATGCCGGCTTCATCTCCCCTTTCTTCCACGCCATCAGTAAAGCAATGGAAGGCACATTTCCGGCAATGGGACGAAAAACAACCTGTCCGGTATTAAAATTATT  >  NC_000913/2667900‑2668161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑