Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,886,788	A→T	H144Q (CAT→CAA)	cas3 ←	CRISPR‑associated endonuclease/helicase Cas3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,886,788	0	A	T	100.0%	297.7 / NA	83	H144Q (CAT→CAA)	cas3	CRISPR‑associated endonuclease/helicase Cas3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (43/40);  total (43/40)

CCTGTTTATCTTGCGCAGCATAAGATGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCA  >  NC_000913/2886652‑2886914                                                                                                                                         |                                                                                                                               ccTGTTTATCTTGCGCAGCATAAGATGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTga                                                                                                                             >  1:205702/1‑140 (MQ=255)            tGCGCAGCATAAGATGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATc                                                                                                                  >  1:107639/1‑140 (MQ=255)               gcagcaTAAGATGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCaaa                                                                                                               >  1:519190/1‑140 (MQ=255)               gcagcaTAAGATGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCaaa                                                                                                               >  1:422224/1‑140 (MQ=255)                         aTGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGaaa                                                                                                     <  2:107639/140‑1 (MQ=255)                             cAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAAt                                                                                                 <  1:1035027/140‑1 (MQ=255)                             cAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAAt                                                                                                 >  1:86044/1‑140 (MQ=255)                             cAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAAt                                                                                                 >  2:627616/1‑140 (MQ=255)                                    gCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCgag                                                                                          >  2:650480/1‑140 (MQ=255)                                       ggCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCgagaga                                                                                       <  2:205702/140‑1 (MQ=255)                                               ccAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTg                                                                               <  2:417863/140‑1 (MQ=255)                                                        cTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAAt                                                                      >  2:885524/1‑140 (MQ=255)                                                         ttATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATc                                                                     <  1:1106678/140‑1 (MQ=255)                                                         ttATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATc                                                                     <  1:420716/140‑1 (MQ=255)                                                             cTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTgg                                                                 >  1:665978/1‑140 (MQ=255)                                                              ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTGACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                <  2:690559/140‑1 (MQ=255)                                                              ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                <  2:1046008/140‑1 (MQ=255)                                                                gATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTa                                                              <  1:885524/140‑1 (MQ=255)                                                                gATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTa                                                              <  1:605686/140‑1 (MQ=255)                                                                   ccTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAAc                                                           <  1:650480/140‑1 (MQ=255)                                                                      gggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGGGAGATTGCTCTGAAAGTGAATCCTGGt                                                                <  1:683081/132‑1 (MQ=255)                                                                      gggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGGGAGATTGCTCTGAAAGTGAATCCTGGt                                                                >  2:683081/1‑132 (MQ=255)                                                                      gggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCaa                                                        >  2:129872/1‑140 (MQ=255)                                                                      gggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCaa                                                        >  2:809195/1‑140 (MQ=255)                                                                           tGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAg                                                   >  2:1000109/1‑140 (MQ=255)                                                                            gTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAAc                                                           <  1:547594/131‑1 (MQ=255)                                                                            gTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAAc                                                           >  2:547594/1‑131 (MQ=255)                                                                             tAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGAc                                                 <  1:627616/140‑1 (MQ=255)                                                                                tataAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCgg                                              >  2:355161/1‑140 (MQ=255)                                                                                   aaaaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTg                                           >  2:665543/1‑140 (MQ=255)                                                                                   aaaaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTg                                           >  2:664572/1‑140 (MQ=255)                                                                                     aaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCa                                         >  1:877141/1‑140 (MQ=255)                                                                                     aaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCa                                         >  2:647236/1‑140 (MQ=255)                                                                                     aaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCa                                         <  1:809195/140‑1 (MQ=255)                                                                                     aaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGACATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCa                                         >  1:297991/1‑140 (MQ=255)                                                                                      aCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAc                                        >  1:67217/1‑140 (MQ=255)                                                                                       ccATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATGAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGAcc                                                <  2:700292/131‑1 (MQ=255)                                                                                       ccATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGAcc                                                >  1:700292/1‑131 (MQ=255)                                                                                          tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAc                                        <  1:1155179/136‑1 (MQ=255)                                                                                          tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAc                                        >  2:1155179/1‑136 (MQ=255)                                                                                          tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                    >  1:1137878/1‑140 (MQ=255)                                                                                          tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                    >  2:1058869/1‑140 (MQ=255)                                                                                          tgatgTCCTGTAACGACCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                    >  1:307731/1‑140 (MQ=255)                                                                                                 cTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAAt                             >  1:501748/1‑140 (MQ=255)                                                                                                    tAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTa                          <  2:877141/140‑1 (MQ=255)                                                                                                    tAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTa                          >  1:280094/1‑140 (MQ=255)                                                                                                     aaCGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTAc                         <  1:664572/140‑1 (MQ=255)                                                                                                      aCGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACg                        <  2:422224/140‑1 (MQ=255)                                                                                                       cGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       <  1:495033/140‑1 (MQ=255)                                                                                                       cGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       <  2:689607/140‑1 (MQ=255)                                                                                                       cGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       >  2:1120618/1‑140 (MQ=255)                                                                                                       cGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       <  2:1137878/140‑1 (MQ=255)                                                                                                       cGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       >  2:116598/1‑140 (MQ=255)                                                                                                       cGGCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       <  2:86044/140‑1 (MQ=255)                                                                                                         gCCTCTACCTATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       <  1:822017/138‑1 (MQ=255)                                                                                                         gCCTCTACCTATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       >  2:822017/1‑138 (MQ=255)                                                                                                         gCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                    <  1:789421/125‑1 (MQ=255)                                                                                                         gCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                    >  2:789421/1‑125 (MQ=255)                                                                                                         gCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                     >  1:662941/1‑140 (MQ=255)                                                                                                         gCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                     >  1:1043342/1‑140 (MQ=255)                                                                                                         gCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                     <  1:116598/140‑1 (MQ=255)                                                                                                         gCCTCTACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                     >  2:977460/1‑140 (MQ=255)                                                                                                           ctctACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGcaca                   >  1:357360/1‑140 (MQ=255)                                                                                                              tACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       <  2:375982/133‑1 (MQ=255)                                                                                                              tACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                       >  1:375982/1‑133 (MQ=255)                                                                                                              tACCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACAttt                >  1:908622/1‑140 (MQ=255)                                                                                                               aCCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTtg               <  1:977460/140‑1 (MQ=255)                                                                                                               aCCCATGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTtg               <  2:665978/140‑1 (MQ=255)                                                                                                                    tGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGcaca                   >  1:1084861/1‑131 (MQ=255)                                                                                                                    tGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGcaca                   <  2:1084861/131‑1 (MQ=255)                                                                                                                    tGGAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTg          >  2:809183/1‑140 (MQ=255)                                                                                                                     ggAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGa         >  1:78064/1‑140 (MQ=255)                                                                                                                      gAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt        <  2:519190/140‑1 (MQ=255)                                                                                                                      gAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt        <  1:355161/140‑1 (MQ=255)                                                                                                                      gAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt        <  1:1000109/140‑1 (MQ=255)                                                                                                                      gAAACCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt        <  1:809183/140‑1 (MQ=255)                                                                                                                       aaaCCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATg       <  1:921529/140‑1 (MQ=255)                                                                                                                        aaCCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg      >  1:33926/1‑140 (MQ=255)                                                                                                                        aaCCAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg      <  2:280094/140‑1 (MQ=255)                                                                                                                          ccAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCGATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGAc    <  1:129872/140‑1 (MQ=255)                                                                                                                          ccAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg      >  1:232712/1‑138 (MQ=255)                                                                                                                          ccAGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg      <  2:232712/138‑1 (MQ=255)                                                                                                                            aGGACTCATAAGGTTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCa  <  1:665543/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                               CCTGTTTATCTTGCGCAGCATAAGATGCCAGAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCA  >  NC_000913/2886652‑2886914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑