Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,031,909	T→C	N181N (AAT→AAC)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,031,909	0	T	C	100.0%	204.2 / NA	55	N181N (AAT→AAC)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

GCGCCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGG  >  NC_000913/3031772‑3032035                                                                                                                                          |                                                                                                                               gcgcCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTGAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAAccc                                                                                                                              >  2:828231/1‑140 (MQ=255) gcgcCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAAccc                                                                                                                              >  2:231998/1‑140 (MQ=255) gcgcCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAAccc                                                                                                                              >  1:385444/1‑140 (MQ=255)   gcCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCg                                                                                                                            <  1:267363/140‑1 (MQ=255)    ccACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGt                                                                                                                           >  1:65868/1‑140 (MQ=255)    ccACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGt                                                                                                                           >  2:840345/1‑140 (MQ=255)      aCTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGttt                                                                                                                         >  2:88651/1‑140 (MQ=255)         tATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGtttatt                                                                                                                      <  2:906509/140‑1 (MQ=255)         tATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGtttatt                                                                                                                      <  2:65868/140‑1 (MQ=255)             ggTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAgg                                                                                                                  <  2:358818/140‑1 (MQ=255)              gTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGt                                                                                                                 <  2:156209/140‑1 (MQ=255)                cGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTAt                                                                                                               <  1:231998/140‑1 (MQ=255)                 gCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTAtc                                                                                                              >  2:18764/1‑140 (MQ=255)                  cTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTAtct                                                                                                             >  1:499354/1‑140 (MQ=255)                         ccTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGc                                                                                                      <  2:499354/140‑1 (MQ=255)                               ttCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTGAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCtt                                                                                                <  1:828231/140‑1 (MQ=255)                                    gCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAAttt                                                                                           >  1:165934/1‑140 (MQ=255)                                    gCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAAttt                                                                                           >  1:674641/1‑140 (MQ=255)                                         tGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATc                                                                                      <  1:189150/140‑1 (MQ=255)                                         tGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATc                                                                                      <  2:437938/140‑1 (MQ=255)                                             tACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGc                                                                                  <  1:154976/140‑1 (MQ=255)                                                cGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTa                                                                                        <  2:820104/131‑1 (MQ=255)                                                cGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTa                                                                                        >  1:820104/1‑131 (MQ=255)                                                cGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATc                                                                                      <  1:1077062/133‑1 (MQ=255)                                                cGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATc                                                                                      >  2:1077062/1‑133 (MQ=255)                                                   tgtgGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTa                                                                            <  2:385444/140‑1 (MQ=255)                                                     tgGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACt                                                                          >  2:165782/1‑140 (MQ=255)                                                     tgGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACt                                                                          >  1:374978/1‑140 (MQ=255)                                                       gTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCg                                                                        >  2:662085/1‑140 (MQ=255)                                                             aCTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGc                                                                  >  2:142538/1‑140 (MQ=255)                                                                 cTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGaaa                                                              >  2:374128/1‑140 (MQ=255)                                                                    tCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGa                                                           >  2:1049878/1‑140 (MQ=255)                                                                         cTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTAt                                                      >  2:737302/1‑140 (MQ=255)                                                                         cTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTAt                                                      >  2:617115/1‑140 (MQ=255)                                                                               atcatTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCaa                                                <  1:617115/140‑1 (MQ=255)                                                                                  atTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGt                                             <  1:737302/140‑1 (MQ=255)                                                                                  atTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGt                                             <  2:165934/140‑1 (MQ=255)                                                                                       ggTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCg                                        <  1:165782/140‑1 (MQ=255)                                                                                       ggTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCg                                        <  2:648713/140‑1 (MQ=255)                                                                                        gTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCg                                        <  1:408400/139‑1 (MQ=255)                                                                                        gTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCg                                        >  2:408400/1‑139 (MQ=255)                                                                                           ggTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTAtt                                    >  2:660303/1‑140 (MQ=255)                                                                                              aTTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACt                                 <  1:662085/140‑1 (MQ=255)                                                                                                     gggCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGAtt                          >  1:329594/1‑140 (MQ=255)                                                                                                      ggCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTg                         >  2:274397/1‑140 (MQ=255)                                                                                                      ggCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTg                         >  2:17358/1‑140 (MQ=255)                                                                                                      ggCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTg                         >  1:138309/1‑140 (MQ=255)                                                                                                       gccgccGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTa                                      <  1:407715/126‑1 (MQ=255)                                                                                                       gccgccGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTa                                      >  2:407715/1‑126 (MQ=255)                                                                                                       gccgccGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGt                        >  2:776182/1‑140 (MQ=255)                                                                                                       gccgccGGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGt                        >  1:957215/1‑140 (MQ=255)                                                                                                           ccgGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTg                         >  1:1042055/1‑135 (MQ=255)                                                                                                           ccgGAGGTAAAGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTg                         <  2:1042055/135‑1 (MQ=255)                                                                                                                   aaaGGGAATCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCttttt            <  1:142538/140‑1 (MQ=255)                                                                                                                          aTCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCg     <  1:840345/140‑1 (MQ=255)                                                                                                                           tCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCt                <  2:431325/128‑1 (MQ=255)                                                                                                                           tCCGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCt                >  1:431325/1‑128 (MQ=255)                                                                                                                             cGCAATATGGTAACCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTgg  <  2:138309/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               GCGCCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGG  >  NC_000913/3031772‑3032035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑