Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,088,177	T→C	intergenic (+317/‑107)	metK → / → galP	methionine adenosyltransferase/galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,088,177	0	T	C	100.0%	184.5 / NA	50	intergenic (+317/‑107)	metK/galP	methionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

TTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGG  >  NC_000913/3088048‑3088310                                                                                                                                  |                                                                                                                                      ttttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAgt                                                                                                                             >  1:588149/1‑140 (MQ=255) ttttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAgt                                                                                                                             >  1:153202/1‑140 (MQ=255)    tCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTa                                                                                                                          <  1:1063815/140‑1 (MQ=255)    tCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTa                                                                                                                          <  2:19131/140‑1 (MQ=255)    tCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTa                                                                                                                          <  1:981560/140‑1 (MQ=255)              attCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTaca                                                                                                                <  2:780383/140‑1 (MQ=255)                    ttttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGAtg                                                                                                          <  2:437874/140‑1 (MQ=255)                      ttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGAtgtg                                                                                                        >  2:487317/1‑140 (MQ=255)                             ttgttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCt                                                                                                 >  1:887709/1‑140 (MQ=255)                                  aCATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTcaca                                                                                            <  1:487317/140‑1 (MQ=255)                                  aCATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTcaca                                                                                            >  2:11830/1‑140 (MQ=255)                                     tttCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCt                                                                                         >  1:573569/1‑140 (MQ=255)                                       tCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCttt                                                                                       >  1:399013/1‑140 (MQ=255)                                       tCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCttt                                                                                       <  1:88282/140‑1 (MQ=255)                                           ttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAc                                                                                   >  2:893782/1‑140 (MQ=255)                                            tCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAcg                                                                                  <  2:414596/140‑1 (MQ=255)                                             cAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgt                                                                                 >  2:380802/1‑140 (MQ=255)                                                 aaaGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTa                                                                             <  1:893782/140‑1 (MQ=255)                                                  aaGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTAc                                                                            <  2:848232/140‑1 (MQ=255)                                                      cTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCAc                                                                        <  2:1126028/140‑1 (MQ=255)                                                       ttAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCAcc                                                                       <  2:564099/140‑1 (MQ=255)                                                        tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCt                                                                      >  2:646112/1‑140 (MQ=255)                                                         aaTTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTa                                                                     <  2:588149/140‑1 (MQ=255)                                                            tGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCt                                                                  <  1:380802/140‑1 (MQ=255)                                                               aGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTaa                                                               >  1:1087083/1‑140 (MQ=255)                                                                gATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                              >  2:741125/1‑140 (MQ=255)                                                                      aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACaa                                                        >  2:797434/1‑140 (MQ=255)                                                                       gCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAAt                                                       <  1:11830/140‑1 (MQ=255)                                                                        cGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATa                                                      >  2:530483/1‑140 (MQ=255)                                                                        cGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATa                                                      >  1:204219/1‑140 (MQ=255)                                                                              aTCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAAt                                                <  1:741125/140‑1 (MQ=255)                                                                              aTCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAAt                                                <  1:530483/140‑1 (MQ=255)                                                                                    gatgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCat                                          >  1:1022895/1‑140 (MQ=255)                                                                                      tgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCatat                                        <  2:1087083/140‑1 (MQ=255)                                                                                         tataACTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAAGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTgg                                     >  1:688194/1‑140 (MQ=255)                                                                                             aCTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAggg                                 >  2:757317/1‑140 (MQ=255)                                                                                             aCTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgga                                 >  1:604635/1‑139 (MQ=255)                                                                                              cTCAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGc                                >  2:150290/1‑140 (MQ=255)                                                                                                cAATTATTTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGGGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcat                              >  2:791988/1‑140 (MQ=255)                                                                                                  attattTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcatca                            >  1:147669/1‑140 (MQ=255)                                                                                                  attattTTCATGCACTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcatca                            >  1:305209/1‑140 (MQ=255)                                                                                                                cTTAAATCATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaaa              >  1:659550/1‑140 (MQ=255)                                                                                                                       cATAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGNATCATGCCTGACGCTAAAAAACAgg       <  2:573569/140‑1 (MQ=255)                                                                                                                        aTAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAggg      <  1:150290/140‑1 (MQ=255)                                                                                                                         tAACTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAgggg     >  1:327731/1‑140 (MQ=255)                                                                                                                          aaCTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc    <  2:204219/140‑1 (MQ=255)                                                                                                                           aCTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc    >  2:188150/1‑139 (MQ=255)                                                                                                                           aCTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc    <  1:188150/139‑1 (MQ=255)                                                                                                                           aCTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCg   >  2:785397/1‑140 (MQ=255)                                                                                                                            cTAAGACAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCgg  <  1:455379/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      TTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGG  >  NC_000913/3088048‑3088310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑