Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,680	(C)6→7	coding (56/966 nt)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,674	1	.	C	100.0%	174.2 / NA	49	A21G (GCG→GGG)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/29);  total (20/29)

CGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAA  >  NC_000913/3332536‑3332808                                                                                                                                            |                                                                                                                                       cGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑c                                                                                                                                       >  1:941240/1‑140 (MQ=255)      cacGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑cccccc                                                                                                                                  <  2:221956/140‑1 (MQ=255)       acGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑ccccccc                                                                                                                                 >  1:1015128/1‑139 (MQ=255)       acGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑c                                                                                                                                       >  1:110839/1‑134 (MQ=255)       acGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑c                                                                                                                                       <  2:110839/134‑1 (MQ=255)           gTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑cccccccagca                                                                                                                             <  2:1015128/140‑6 (MQ=255)           gTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑cccccccagca                                                                                                                             <  2:909395/140‑6 (MQ=255)             aaTGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCaaa                                                                                                                            <  1:596813/140‑1 (MQ=255)              aTGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAAt                                                                                                                           >  2:611808/1‑140 (MQ=255)                ggcggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTg                                                                                                                         >  2:1051555/1‑140 (MQ=255)                  cggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCt                                                                                                                       <  2:127196/140‑1 (MQ=255)                   ggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAGAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCtg                                                                                                                      >  1:124119/1‑140 (MQ=255)                         ctctTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGa                                                                                                                >  1:263639/1‑140 (MQ=255)                              cttcACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGcc                                                                                                           <  2:330928/140‑1 (MQ=255)                                      cAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaat                                                                                                   >  2:475075/1‑140 (MQ=255)                                      cAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaat                                                                                                   >  2:701278/1‑140 (MQ=255)                                              gCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaatgccaatgc                                                                                           >  2:37386/1‑140 (MQ=255)                                              gCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaatgccaatgc                                                                                           >  2:668083/1‑140 (MQ=255)                                                   cAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctgcc                                                                                      <  1:387205/140‑1 (MQ=255)                                                      gCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCctgcctgc                                                                                   >  1:998663/1‑140 (MQ=255)                                                       ccAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCctgct                                                                                  <  2:1143082/140‑1 (MQ=255)                                                          aTACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                                               <  2:552597/140‑1 (MQ=255)                                                          aTACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                                               <  2:263639/140‑1 (MQ=255)                                                            aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                             <  1:725015/140‑1 (MQ=255)                                                            aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                             <  1:668083/140‑1 (MQ=255)                                                            aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                             <  1:611808/140‑1 (MQ=255)                                                              tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                           <  1:1051555/140‑1 (MQ=255)                                                              tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                           <  2:719184/140‑1 (MQ=255)                                                              tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                           <  1:37386/140‑1 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                    <  1:777063/129‑1 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                    >  2:777063/1‑129 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                                                         >  2:994895/1‑140 (MQ=255)                                                                          aaCGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCt                                                               >  2:544567/1‑140 (MQ=255)                                                                             ggTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCa                                                            <  2:941240/140‑1 (MQ=255)                                                                                       gATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCAttt                                                  <  2:998663/140‑1 (MQ=255)                                                                                       gATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCAttt                                                  <  1:835589/140‑1 (MQ=255)                                                                                             cGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                            <  1:475075/140‑1 (MQ=255)                                                                                             cGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                            <  1:838004/140‑1 (MQ=255)                                                                                               gAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTa                                          <  1:994895/140‑1 (MQ=255)                                                                                                            cTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg                             <  2:187255/140‑1 (MQ=255)                                                                                                             tCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgc                            >  2:1029900/1‑140 (MQ=255)                                                                                                              ccAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg                           >  2:615131/1‑140 (MQ=255)                                                                                                              ccAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg                           <  1:615131/140‑1 (MQ=255)                                                                                                                  cACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt                       >  2:755460/1‑140 (MQ=255)                                                                                                                  cACCTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt                       >  2:753601/1‑140 (MQ=255)                                                                                                                    ccTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt                     <  2:124119/140‑1 (MQ=255)                                                                                                                     cTGCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt                    >  1:628297/1‑140 (MQ=255)                                                                                                                       gCTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc                  <  1:1016804/140‑1 (MQ=255)                                                                                                                        cTTCATTGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAcc                 >  2:33997/1‑140 (MQ=255)                                                                                                                              tGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt           <  2:883633/140‑1 (MQ=255)                                                                                                                              tGCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTACTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt           <  2:710914/140‑1 (MQ=255)                                                                                                                               gCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATc          <  1:753601/140‑1 (MQ=255)                                                                                                                               gCGATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATc          <  1:755460/140‑1 (MQ=255)                                                                                                                                 gATTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa        <  1:33997/140‑1 (MQ=255)                                                                                                                                  aTTGGCAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa       >  2:119983/1‑140 (MQ=255)                                                                                                                                       cAACGCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaa  >  1:493473/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       CGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAA  >  NC_000913/3332536‑3332808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑