| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,826,470 | (A)8→9 | coding (79/960 nt) | srlE → | sorbitol‑specific PTS enzyme IIBC1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,826,462 | 1 | . | A | 79.2% | 63.4 / 11.5 | 24 | coding (71/960 nt) | srlE | sorbitol‑specific PTS enzyme IIBC1 component |
| Reads supporting (aligned to +/- strand): ref base . (0/5); new base A (14/5); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.93e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
CGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAA > NC_000913/2826326‑2826596 | cGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaa > 1:544264/1‑140 (MQ=255) ccATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaaaa > 1:1088041/1‑140 (MQ=255) tttttGAGAAAAAGATGGGCATTCAACTTGAACAAAAGGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaaaaaaa > 1:515123/1‑140 (MQ=255) ttttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaaaaaaaa < 2:515123/140‑2 (MQ=255) ttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaaaaaaaatc < 2:883255/140‑4 (MQ=255) ttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaaaaaaaatc < 2:1088041/140‑4 (MQ=255) tGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑aaaaaaaaatcg < 2:458476/140‑5 (MQ=255) aaaaaGATGGGCATTCAACTTGAACAAAAAGTTCACCGGGCAGGAGCAACAGCATGACGCATATGCGGATCGAAAAAGGAAC‑GGGGGGCGGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCta < 2:467001/140‑1 (MQ=255) aaaaGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCtat < 1:574850/140‑1 (MQ=255) taaaCTTAAAAAAAAAGTTCCCCGGGCGGGAGAAACATCAGGACGCATATTCGAATC‑AAAAAGGAACGGGGGGGCGGGGGCGGCCCCCTT‑GAGC‑GAAAGCCCCCGCGG‑GAAAAAAAAAAGCGTCTATATCACCGCCGGTa < 2:224797/138‑1 (MQ=255) tCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTa > 2:778922/1‑140 (MQ=255) aCAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTg > 1:748029/1‑140 (MQ=255) aaGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGAtt > 1:915391/1‑140 (MQ=255) ccccgggCGGGAGAAACATCATGCCGCATATTGGGATCAAAAAAGGAAG‑GGGGGGCGGGGGCGGCCCGTTT‑GAGCGAAAAGCCACGCGGGAA‑‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGa < 2:544264/135‑1 (MQ=255) gaaaaaCACCATGACGCATTTTGGAAAAAAAAAAGGAAG‑GGGGGGGGGGGGC‑GCCCGCTTGGGGCGAAAAGCCCCGGGGGAA‑‑AAAAAAAATCGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGca < 2:546121/136‑1 (MQ=255) gAGCAACATCAGGCCGCATATCCGGATAAAAAAAGGAAG‑GGGTGGCGGGGGCGCCCCGCTT‑GAGCTAAAACCCACGCCGGAA‑‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGca < 2:748029/140‑1 (MQ=255) catcatGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCtt > 1:495339/1‑140 (MQ=255) tatTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAg > 1:678342/1‑140 (MQ=255) tatTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAg > 1:1056775/1‑140 (MQ=255) atTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAgg > 2:1107518/1‑140 (MQ=255) gggataaaaaaaGGAAG‑GGGTGGCGGGGGCGCCCCGCTT‑GAGCTAAAACCCCCGCGGGAA‑‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTa < 2:678342/133‑1 (MQ=255) gATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTAtt > 2:747687/1‑140 (MQ=255) aTCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTg > 2:1030812/1‑140 (MQ=255) aTCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTg > 1:64149/1‑140 (MQ=255) taaaaaaaGGAAC‑GGGTGGCGGGGGCGCCCCGCTT‑GAGCTAAAAGCCACGCGGGAA‑‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa < 2:915391/137‑1 (MQ=255) aaaaaGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACgg > 1:579200/1‑140 (MQ=255) cccGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAg > 2:3392/1‑140 (MQ=255) cccGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAg > 1:627699/1‑140 (MQ=255) cccGCTT‑GAGCTGAAAGCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAg > 1:576658/1‑140 (MQ=255) gCCACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg < 2:1056775/140‑1 (MQ=255) ccACGCCGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTCACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcgc < 1:1107518/140‑1 (MQ=255) ccGG‑GA‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTaa < 1:3392/140‑1 (MQ=255) | CGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGGCGGCCCGCTT‑GAGCTGAAAGCCACGCCGG‑G‑‑AAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAA > NC_000913/2826326‑2826596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |