Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,526	A→G	V712V (GTA→GTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,526	0	A	G	100.0%	257.0 / NA	72	V712V (GTA→GTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/37);  total (35/37)

TTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACA  >  NC_000913/24396‑24653                                                                                                                                   |                                                                                                                                ttGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTg                                                                                                                        >  1:348655/1‑140 (MQ=255)    aTCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATg                                                                                                                     <  2:653166/140‑1 (MQ=255)         gCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCtt                                                                                                                >  1:689585/1‑140 (MQ=255)         gCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCtt                                                                                                                >  2:941043/1‑140 (MQ=255)            aaaGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTTAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTg                                                                                                             <  2:525357/140‑1 (MQ=255)            aaaGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTg                                                                                                             <  2:106126/140‑1 (MQ=255)            aaaGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTg                                                                                                             <  2:689585/140‑1 (MQ=255)                    ggTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTg                                                                                                     >  2:291860/1‑140 (MQ=255)                       gAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTgaga                                                                                                  <  1:688062/140‑1 (MQ=255)                       gAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTgaga                                                                                                  <  1:194602/140‑1 (MQ=255)                          aCCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTgag                                                                                                   >  2:491135/1‑136 (MQ=255)                          aCCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTgag                                                                                                   <  1:491135/136‑1 (MQ=255)                           ccGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATggg                                                                                              >  1:1034613/1‑140 (MQ=255)                               aaGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTcc                                                                                          >  2:743706/1‑140 (MQ=255)                                agagATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCt                                                                                         <  2:400179/140‑1 (MQ=255)                                    aTGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTa                                                                                     >  2:816671/1‑140 (MQ=255)                                      ggtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTAcc                                                                                   >  1:555530/1‑140 (MQ=255)                                       gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCt                                                                                  <  2:669947/140‑1 (MQ=255)                                       gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCt                                                                                  <  1:171689/140‑1 (MQ=255)                                         ggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATATGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCg                                                                                >  2:524257/1‑140 (MQ=255)                                          gtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATATGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGa                                                                               <  1:524257/140‑1 (MQ=255)                                           tACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAc                                                                              <  2:348655/140‑1 (MQ=255)                                            aCTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAca                                                                             <  1:228814/140‑1 (MQ=255)                                            aCTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAca                                                                             <  1:696229/140‑1 (MQ=255)                                             cTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAcat                                                                            >  1:745440/1‑140 (MQ=255)                                             cTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAcat                                                                            >  2:977899/1‑140 (MQ=255)                                              tGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTT‑AGATGGGTTCCTTCTACCTCGAcatca                                                                          >  2:198595/1‑140 (MQ=255)                                                gATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAAGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAcatcat                                                                         <  2:1115583/140‑1 (MQ=255)                                                   cGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCaa                                                                      >  1:121586/1‑140 (MQ=255)                                                     cTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAg                                                                    >  1:53158/1‑140 (MQ=255)                                                     cTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAg                                                                    <  1:941043/140‑1 (MQ=255)                                                     cTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAg                                                                    >  2:862178/1‑140 (MQ=255)                                                     cTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAg                                                                    <  2:1138039/140‑1 (MQ=255)                                                       gggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGAc                                                                  <  2:385800/140‑1 (MQ=255)                                                       gggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGAc                                                                  <  2:353353/140‑1 (MQ=255)                                                       gggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGAc                                                                  <  1:977899/140‑1 (MQ=255)                                                        ggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGAcc                                                                 <  1:816671/140‑1 (MQ=255)                                                             tAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAg                                                            >  2:947874/1‑140 (MQ=255)                                                             tAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAg                                                            >  1:272301/1‑140 (MQ=255)                                                                  tgtgCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTacac                                                       >  1:112582/1‑140 (MQ=255)                                                                    tgCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCg                                                     >  2:661213/1‑140 (MQ=255)                                                                    tgCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCg                                                     >  1:177575/1‑140 (MQ=255)                                                                    tgCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCg                                                     >  2:867538/1‑140 (MQ=255)                                                                    tgCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCg                                                     >  2:3909/1‑140 (MQ=255)                                                                      cGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGcc                                                   <  1:291860/140‑1 (MQ=255)                                                                       gAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCa                                                  <  2:171723/140‑1 (MQ=255)                                                                                           tCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTT‑AGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGcgtc                             <  1:198595/140‑1 (MQ=255)                                                                                            ccTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGcgtc                             >  2:17708/1‑140 (MQ=255)                                                                                              tCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGcgtcgt                           >  2:968156/1‑140 (MQ=255)                                                                                              tCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGcgtcgt                           <  2:745440/140‑1 (MQ=255)                                                                                              tCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGcgtcgt                           <  1:918332/140‑1 (MQ=255)                                                                                              tCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGcgtcgt                           >  2:825825/1‑140 (MQ=255)                                                                                               cAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTa                          >  1:783701/1‑140 (MQ=255)                                                                                                aaGGCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAg                         <  1:968156/140‑1 (MQ=255)                                                                                                  ggCGTACGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCt                       >  1:492108/1‑140 (MQ=255)                                                                                                      tACGAAGCATACGATTTCCACGAAGT‑GTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAg                  >  1:162585/1‑140 (MQ=255)                                                                                                        cGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGa                 <  2:492108/140‑1 (MQ=255)                                                                                                        cGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGa                 >  1:302281/1‑140 (MQ=255)                                                                                                        cGAAGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGa                 >  1:996499/1‑140 (MQ=255)                                                                                                           aGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTg              <  1:3909/140‑1 (MQ=255)                                                                                                           aGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTg              <  1:743706/140‑1 (MQ=255)                                                                                                           aGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTg              <  1:862178/140‑1 (MQ=255)                                                                                                           aGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTg              <  1:947874/140‑1 (MQ=255)                                                                                                           aGCATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTg              <  2:302281/140‑1 (MQ=255)                                                                                                             cATACGATTTCCACGAAGTGGTGCAGCGTCTGCTGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCACAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTgcg            <  2:1034613/140‑1 (MQ=255)                                                                                                             cATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTgcg            <  2:175372/140‑1 (MQ=255)                                                                                                             cATACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTgcg            <  2:121586/140‑1 (MQ=255)                                                                                                               tACGATTTCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCt          >  1:64844/1‑140 (MQ=255)                                                                                                                     ttCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATca    >  1:983157/1‑140 (MQ=255)                                                                                                                     ttCCACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATca    <  2:168641/140‑1 (MQ=255)                                                                                                                       ccACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATcaca  >  1:354814/1‑140 (MQ=255)                                                                                                                       ccACGAAGTGGTGCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATcaca  >  1:651102/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                TTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACA  >  NC_000913/24396‑24653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑